Patents by Inventor Norman Dovichi
Norman Dovichi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11746388Abstract: A system and methods of characterizing a population of a virus, bacteriophage, or other microbes in a microbiome that includes the steps of separating a sample of microbiota into more than one fraction by continuous capillary zone electrophoresis based on the physiochemical properties of the microbes in the microbiota using a constant voltage applied to the sample during the continuous zone electrophoresis. At least one separated fraction includes an intact virus, bacteriophage, or other microbe that may be visualized and/or directly sequenced to characterize the population of the virus, bacteriophage, or other microbe in the microbiome.Type: GrantFiled: August 6, 2020Date of Patent: September 5, 2023Assignee: University of Notre Dame Du LacInventors: Norman Dovichi, Bonnie Jaskowski Huge, Matthew Champion
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Patent number: 11056329Abstract: An electrokinetically pumped sheath flow nanospray interface for capillary electrophoresis coupled to negative mode electrospray mass spectrometer is described. At this interface, application of an electric field generates electro-osmotic flow at the interior of a glass emitter having an orifice. Electroosmotic flow pumps liquid around the distal tip of the separation capillary, ensheathing analyte into the electrospray electrolyte. In negative ion mode, negative potential applied to an untreated emitter drives sheath flow away from the emitter orifice, decreasing the stability and efficiency of the spray. In contrast, when the interior of the electrospray emitter is grafted with aminoalkylsilanes, the amines have a positive charge, which reverses electroosmosis and generates stable sheath flow to the emitter orifice under negative potential. Limits of detection were about 150 to 900 attomoles injected.Type: GrantFiled: December 30, 2016Date of Patent: July 6, 2021Assignee: University of Notre Dame du LacInventors: Norman Dovichi, Scott Sarver, Nicole Schiavone, Zhenbin Zhang, Liangliang Sun
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Publication number: 20200385787Abstract: A system and methods of characterizing a population of a virus, bacteriophage, or other microbes in a microbiome that includes the steps of separating a sample of microbiota into more than one fraction by continuous capillary zone electrophoresis based on the physiochemical properties of the microbes in the microbiota using a constant voltage applied to the sample during the continuous zone electrophoresis. At least one separated fraction includes an intact virus, bacteriophage, or other microbe that may be visualized and/or directly sequenced to characterize the population of the virus, bacteriophage, or other microbe in the microbiome.Type: ApplicationFiled: August 6, 2020Publication date: December 10, 2020Applicant: University of Notre Dame du LacInventors: Norman DOVICHI, Bonnie JASKOWSKI HUGE, Matthew CHAMPION
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Publication number: 20200273693Abstract: An electrokinetically pumped sheath flow nanospray interface for capillary electrophoresis coupled to negative mode electrospray mass spectrometer is described. At this interface, application of an electric field generates electro-osmotic flow at the interior of a glass emitter having an orifice. Electroosmotic flow pumps liquid around the distal tip of the separation capillary, ensheathing analyte into the electrospray electrolyte. In negative ion mode, negative potential applied to an untreated emitter drives sheath flow away from the emitter orifice, decreasing the stability and efficiency of the spray. In contrast, when the interior of the electrospray emitter is grafted with aminoalkylsilanes, the amines have a positive charge, which reverses electroosmosis and generates stable sheath flow to the emitter orifice under negative potential. Limits of detection were about 150 to 900 attomoles injected.Type: ApplicationFiled: December 30, 2016Publication date: August 27, 2020Applicant: University of Notre Dame du LacInventors: Norman DOVICHI, Scott SARVER, Nicole SCHIAVONE, Zhenbin ZHANG, Liangliang SUN
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Publication number: 20200088681Abstract: A surface-confined aqueous reversible addition-fragmentation chain transfer (SCARAFT) polymerization method was developed to coat capillaries for use in capillary zone electrophoresis (CZE). This coating produced an electroosmotic an order of magnitude lower than that of commercial linear polyacrylamide (LPA)-coated capillaries. Coated capillaries were evaluated for bottom-up proteomic analysis using CZE. The very low electroosmotic mobility results in a 200 min separation and improved single-shot analysis. Various types of coatings were prepared by simply changing the functional vinyl monomers in the polymerization mixture.Type: ApplicationFiled: April 18, 2018Publication date: March 19, 2020Applicant: University of Notre Dame du LacInventors: Norman DOVICHI, Zhenbin ZHANG
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Patent number: 10578580Abstract: The invention provides a capillary isoelectric focusing (cIEF) system based on a sandwich injection method for automated chemical mobilization. This system was coupled with an electrokinetically pumped nanoelectrospray interface to a mass spectrometer. The nanoelectrospray emitter employed an acidic sheath electrolyte. To realize automated focusing and mobilization, a plug of ammonium hydroxide was first injected into the capillary, followed by a section of mixed sample and ampholyte. As focusing progressed, the NH3H2O section was titrated to lower pH buffer by the acidic sheath buffer. Chemical mobilization started automatically once the ammonium hydroxide was consumed by the acidic sheath flow electrolyte.Type: GrantFiled: September 22, 2017Date of Patent: March 3, 2020Assignee: University of Notre Dame du LacInventors: Norman Dovichi, Guijie Zhu, Liangliang Sun
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Patent number: 10416115Abstract: A method of coating the inside wall of a capillary with a polymeric material for capillary electrophoresis is disclosed. The method can include introducing a catalyst-free solution of a monomer and initiator, wherein the monomer is present in about 1-10% (w/v) and the initiator is present in 0.1-1% (w/v), into a capillary and thermally initiating polymerization of the monomer thereby providing a capillary comprising an internal polymeric coating for separating, identifying, and quantifying components of an analyte.Type: GrantFiled: June 20, 2016Date of Patent: September 17, 2019Assignee: University of Nortre Dame du LacInventors: Norman Dovichi, Guijie Zhu, Liangliang Sun
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Publication number: 20190004010Abstract: The invention provides a capillary isoelectric focusing (cIEF) system based on a sandwich injection method for automated chemical mobilization. This system was coupled with an electrokinetically pumped nanoelectrospray interface to a mass spectrometer. The nanoelectrospray emitter employed an acidic sheath electrolyte. To realize automated focusing and mobilization, a plug of ammonium hydroxide was first injected into the capillary, followed by a section of mixed sample and ampholyte. As focusing progressed, the NH3H2O section was titrated to lower pH buffer by the acidic sheath buffer. Chemical mobilization started automatically once the ammonium hydroxide was consumed by the acidic sheath flow electrolyte.Type: ApplicationFiled: September 22, 2017Publication date: January 3, 2019Applicant: UNIVERSITY OF NOTRE DAME DU LACInventors: Norman DOVICHI, Guijie ZHU, Liangliang SUN
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Publication number: 20180363054Abstract: Current procedures for the analysis of sexual assault kits are labor intensive, time consuming and deliver a success rate lower than 40%. This has resulted in rape kit backlogs of thousands. The primary challenge crime laboratories face in analyzing these cases is the separation of purified male DNA from the mixture of primarily female DNA from gynecological swabs. Effective elution of the sample from the swab and efficient separation of intact sperm cells from epithelial and other cellular debris, allow for a successful PCR amplification and short tandem repeat (STR) DNA analysis for perpetrator identification. The disclosure provides an effective and economically accessible technology for the separation of male and female cells and DNA, for example, from a gynecological swab by capillary zone electrophoresis (CZE).Type: ApplicationFiled: June 20, 2018Publication date: December 20, 2018Applicant: University of Notre Dame du LacInventors: Sarah LUM, Norman DOVICHI, Bonnie JASKOWSKI HUGE, Carlos Gusti GARTNER
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Publication number: 20180258461Abstract: Capillary electrophoresis fractionation of an environmental microbiota segregates high abundance microbes from lower abundance species, and results in a three-fold increase in the number of 16s rRNA OTUs that map to known species. However, most of the bacteria are found in a few wells, although reasonably large numbers of OTUs were found in over half the wells.Type: ApplicationFiled: March 7, 2018Publication date: September 13, 2018Applicant: University of Notre Dame du LacInventors: Norman Dovichi, Bonnie Jaskowski Huge, Matthew Champion
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Publication number: 20170363576Abstract: The invention provides a novel method of coating the inside of a capillary with a polymeric material. The method can include introducing a catalyst-free solution of a monomer and initiator, wherein the monomer is present in about 1-10% (w/v) and the initiator is present in 0.1-1% (w/v), into a capillary and thermally initiating polymerization of the monomer thereby providing a capillary comprising an internal polymeric coating for separating, identifying, and quantifying components of an analyte.Type: ApplicationFiled: June 20, 2016Publication date: December 21, 2017Applicant: University of Notre Dame du LacInventors: Norman DOVICHI, Guijie ZHU, Liangliang SUN
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Patent number: 9465014Abstract: The invention provides a sheath-flow interface for producing electrospray from a capillary. The electrospray generated by the interface can be used as the source of ions for mass spectrometry. Electrokinetic flow in the interface can move a sheath liquid past the end of a capillary so as to mix with an analyte effluent discharged from the capillary. The sheath liquid and analyte mixture can be directed to an electrospray emitter to generate an electrospray.Type: GrantFiled: April 5, 2015Date of Patent: October 11, 2016Assignee: University of Notre Dame du LacInventors: Norman Dovichi, Liangliang Sun, Guijie Zhu
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Patent number: 9362102Abstract: The invention provides a sheath-flow interface for producing electrospray from a capillary. The electrospray generated by the interface can be used as the source of ions for mass spectrometry. Electrokinetic flow in the interface can move a sheath liquid past the end of a capillary so as to mix with an analyte effluent discharged from the capillary. The sheath liquid and analyte mixture can be directed to an electrospray emitter to generate an electrospray.Type: GrantFiled: July 7, 2015Date of Patent: June 7, 2016Assignee: University of Notre Dame du LacInventors: Norman Dovichi, Liangliang Sun, Guijie Zhu
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Patent number: 9234880Abstract: A sheath-flow interface for producing electrospray from a capillary is provided. The electrospray generated by the interface can be used as the source of ions for mass spectrometry. In the interface, electrokinetic flow moves a sheath liquid past the end of a capillary so as to mix with an analyte effluent discharged from the capillary. The mixture of sheath liquid and analyte is directed to an electrospray emitter in order to generate an electrospray.Type: GrantFiled: June 4, 2012Date of Patent: January 12, 2016Assignee: University of Washington through its Center for CommercializationInventors: Norman Dovichi, Roza Wojcik
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Publication number: 20150311056Abstract: The invention provides a sheath-flow interface for producing electrospray from a capillary. The electrospray generated by the interface can be used as the source of ions for mass spectrometry. Electrokinetic flow in the interface can move a sheath liquid past the end of a capillary so as to mix with an analyte effluent discharged from the capillary. The sheath liquid and analyte mixture can be directed to an electrospray emitter to generate an electrospray.Type: ApplicationFiled: July 7, 2015Publication date: October 29, 2015Applicant: University of Notre Dame de LacInventors: Norman Dovichi, Liangliang Sun, Guijie Zhu
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Patent number: 9159537Abstract: Described herein is a method and system for on-line coupling of capillary isoelectric focusing (cIEF) to high-resolution mass spectrometry in which a sheath flow buffer comprising polar organic solvent and organic acid is used as both an immobilization solution for (cIEF) and an ionization solution for electrospray ionization (ESI).Type: GrantFiled: May 2, 2013Date of Patent: October 13, 2015Assignees: University of Notre Dame du Lac, Medimmune, LLCInventors: James McGivney, Guijie Zhu, Norman Dovichi
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Publication number: 20150233877Abstract: The invention provides a sheath-flow interface for producing electrospray from a capillary. The electrospray generated by the interface can be used as the source of ions for mass spectrometry. Electrokinetic flow in the interface can move a sheath liquid past the end of a capillary so as to mix with an analyte effluent discharged from the capillary. The sheath liquid and analyte mixture can be directed to an electrospray emitter to generate an electrospray.Type: ApplicationFiled: April 5, 2015Publication date: August 20, 2015Applicant: UNIVERSITY OF NOTRE DAME DU LACInventors: Norman Dovichi, Liangliang Sun, Guijie Zhu
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Publication number: 20150162177Abstract: Described herein is a method and system for on-line coupling of capillary isoelectric focusing (cIEF) to high-resolution mass spectrometry in which a sheath flow buffer comprising polar organic solvent and organic acid is used as both an immobilization solution for (cIEF) and an ionization solution for electrospray ionization (ESI).Type: ApplicationFiled: May 2, 2013Publication date: June 11, 2015Inventors: James McGivney, Guijie Zhu, Norman Dovichi
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Publication number: 20130140180Abstract: A sheath-flow interface for producing electrospray from a capillary is provided. The electrospray generated by the interface can be used as the source of ions for mass spectrometry. In the interface, electrokinetic flow moves a sheath liquid past the end of a capillary so as to mix with an analyte effluent discharged from the capillary. The mixture of sheath liquid and analyte is directed to an electrospray emitter in order to generate an electrospray.Type: ApplicationFiled: June 4, 2012Publication date: June 6, 2013Applicant: University of Washington through its Center for CommercializationInventors: Norman Dovichi, Roza Wojcik