Patents by Inventor Nripen Singh
Nripen Singh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11772020Abstract: A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyacrylonitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased binding capacity for soluble impurities such as DNA and host cell proteins from biological/cell culture feedstreams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.Type: GrantFiled: August 16, 2022Date of Patent: October 3, 2023Assignee: EMD Millipore CorporationInventors: Maybelle Woo, David Yavorsky, John Amara, Nripen Singh, Kwok-Shun Cheng
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Patent number: 11660555Abstract: A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyaeryionitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased creased binding capacity for soluble impurities such as DNA and host eel! proteins from biological/cell culture feed-streams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.Type: GrantFiled: September 2, 2014Date of Patent: May 30, 2023Assignee: EMD Millipore CorporationInventors: Maybelle Woo, David Yavorksy, John Amara, Nripen Singh, Kwok-Shun Cheng
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Publication number: 20230158429Abstract: A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyacrylonitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased binding capacity for soluble impurities such as DNA and host cell proteins from biological/cell culture feedstreams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.Type: ApplicationFiled: January 11, 2023Publication date: May 25, 2023Inventors: Maybelle Woo, David Yavorsky, John Amara, Nripen Singh, Kwok-Shun Cheng
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Patent number: 11590439Abstract: A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyaeryionitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased creased binding capacity for soluble impurities such as DNA and host eel! proteins from biological/cell culture feed-streams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.Type: GrantFiled: September 2, 2014Date of Patent: February 28, 2023Assignee: EMD Millipore CorporationInventors: Maybelle Woo, David Yavorksy, John Amara, Nripen Singh, Kwok-Shun Cheng
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Publication number: 20220387917Abstract: A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyacrylonitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased binding capacity for soluble impurities such as DNA and host cell proteins from biological/cell culture feedstreams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.Type: ApplicationFiled: August 16, 2022Publication date: December 8, 2022Inventors: Maybelle Woo, David Yavorsky, John Amara, Nripen Singh, Kwok-Shun Cheng
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Patent number: 11504646Abstract: A process for the primary clarification of feeds, including chemically treated flocculated feeds, containing the target biomolecules of interest such as mAbs, mammalian cell cultures, or bacterial cell cultures, using a primary clarification depth filtration device without the use of a primary clarification centrifugation step or a primary clarification tangential flow microfiltration step. The primary clarification depth filtration device contains a porous depth filter having graded porous layers of varying pore ratings. The primary clarification depth filtration device filters fluid feeds, including chemically treated flocculated feeds containing flocculated cellular debris and colloidal particulates having a particle size distribution of approximately about 0.5 ?m to 200 ?m, at a flow rate of about 10 litres/m2/hr to about 100 litres/m2/hr. Kits and methods of using and making the same are also provided.Type: GrantFiled: September 1, 2020Date of Patent: November 22, 2022Assignee: EMD Millipore CorporationInventors: Nripen Singh, Kwok-Shun Cheng
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Patent number: 11439933Abstract: A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyacrylonitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased binding capacity for soluble impurities such as DNA and host cell proteins from biological/cell culture feedstreams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.Type: GrantFiled: December 19, 2019Date of Patent: September 13, 2022Assignee: EMD Millipore CorporationInventors: Maybelle Woo, David Yavorsky, John Amara, Nripen Singh, Kwok-Shun Cheng
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Publication number: 20220162260Abstract: In certain embodiments, the invention provides a method of purifying a protein of interest from a mixture which comprises the protein of interest and one or more contaminants, comprising: (a) contacting the mixture with a dextran polymer under conditions suitable for the dextran polymer to bind to one or more contaminants, thereby to form a contaminant precipitate; (b) separating the contaminant precipitate from the mixture to form a solution, thereby purifying the protein of interest.Type: ApplicationFiled: February 8, 2022Publication date: May 26, 2022Inventors: Zhijun Tan, Nripen Singh, Mi Jin, Chao Huang, Richard P. Martel, JR., Angela T. Lewandowski, Zhengjian Li
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Publication number: 20210253633Abstract: This disclosure provides a method for concentrating a solution of a macromolecule that is retained on at least two semi-permeable membranes that have different molecular weight cutoffs (MWCOs), the method comprising passing the solution through a hybrid configuration of said semi-permeable membranes staged in series in a single pass tangential flow filtration (SPTFF) apparatus. The method is applicable to the efficient concentration of biological macromolecules such as proteins, antibodies and nucleic acids.Type: ApplicationFiled: June 6, 2019Publication date: August 19, 2021Inventors: Abhiram Arunkumar, Nripen Singh, Junyan Zhang
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Publication number: 20210171573Abstract: This disclosure provides a novel flush method using a recovery flush technique that minimizes yield losses due to inadequate flushing and prevents or reduces dilution of a target protein during a recovery flush.Type: ApplicationFiled: August 13, 2019Publication date: June 10, 2021Inventors: Abhiram Arunkumar, Nripen Singh, Ameya Umesh Borwankar
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Publication number: 20200398187Abstract: A process for the primary clarification of feeds, including chemically treated flocculated feeds, containing the target biomolecules of interest such as mAbs, mammalian cell cultures, or bacterial cell cultures, using a primary clarification depth filtration device without the use of a primary clarification centrifugation step or a primary clarification tangential flow microfiltration step. The primary clarification depth filtration device contains a porous depth filter having graded porous layers of varying pore ratings. The primary clarification depth filtration device filters fluid feeds, including chemically treated flocculated feeds containing flocculated cellular debris and colloidal particulates having a particle size distribution of approximately about 0.5 ?m to 200 ?m, at a flow rate of about 10 litres/m2/hr to about 100 litres/m2/hr. Kits and methods of using and making the same are also provided.Type: ApplicationFiled: September 1, 2020Publication date: December 24, 2020Inventors: Nripen Singh, Kwok-Shun Cheng
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Patent number: 10786754Abstract: A process for the primary clarification of feeds, including chemically treated flocculated feeds, containing the target biomolecules of interest such as mAbs, mammalian cell cultures, or bacterial cell cultures, using a primary clarification depth filtration device without the use of a primary clarification centrifugation step or a primary clarification tangential flow microfiltration step. The primary clarification depth filtration device contains a porous depth filter having graded porous layers of varying pore ratings. The primary clarification depth filtration device filters fluid feeds, including chemically treated flocculated feeds containing flocculated cellular debris and colloidal particulates having a particle size distribution of approximately about 0.5 ?m to 200 ?m, at a flow rate of about 10 litres/m2/hr to about 100 litres/m2/hr. Kits and methods of using and making the same are also provided.Type: GrantFiled: August 8, 2018Date of Patent: September 29, 2020Assignee: EMD Millipore CorporationInventors: Nripen Singh, Kwok-Shun Cheng
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Publication number: 20200129901Abstract: A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyacrylonitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased binding capacity for soluble impurities such as DNA and host cell proteins from biological/cell culture feedstreams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.Type: ApplicationFiled: December 19, 2019Publication date: April 30, 2020Inventors: Maybelle Woo, David Yavorsky, John Amara, Nripen Singh, Kwok-Shun Cheng
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Publication number: 20180345173Abstract: A process for the primary clarification of feeds, including chemically treated flocculated feeds, containing the target biomolecules of interest such as mAbs, mammalian cell cultures, or bacterial cell cultures, using a primary clarification depth filtration device without the use of a primary clarification centrifugation step or a primary clarification tangential flow microfiltration step. The primary clarification depth filtration device contains a porous depth filter having graded porous layers of varying pore ratings. The primary clarification depth filtration device filters fluid feeds, including chemically treated flocculated feeds containing flocculated cellular debris and colloidal particulates having a particle size distribution of approximately about 0.5 ?m to 200 ?m, at a flow rate of about 10 litres/m2/hr to about 100 litres/m2/hr. Kits and methods of using and making the same are also provided.Type: ApplicationFiled: August 8, 2018Publication date: December 6, 2018Inventors: Nripen Singh, Kwok-Shun Cheng
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Publication number: 20180105555Abstract: In certain embodiments, the invention provides a method of purifying a protein of interest from a mixture which comprises the protein of interest and one or more contaminants, comprising: (a) contacting the mixture with a dextran polymer under conditions suitable for the dextran polymer to bind to one or more contaminants, thereby to form a contaminant precipitate; (b) separating the contaminant precipitate from the mixture to form a solution, thereby purifying the protein of interest.Type: ApplicationFiled: March 18, 2016Publication date: April 19, 2018Inventors: Zhijun Tan, Nripen Singh, Mi Jin, Chao Huang, Richard P. Martel, JR., Angela T. Lewandowski, Zhengjian Li
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Patent number: 9731288Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.Type: GrantFiled: November 13, 2015Date of Patent: August 15, 2017Assignee: EMD Millipore CorporationInventors: Jad Jaber, Wilson Moya, James Hamzik, Arezki Boudif, Yu Zhang, Neil Soice, John Charkoudian, Nripen Singh
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Publication number: 20160114272Abstract: A depth filtration device for the clarification of biological fluids including a composite depth filter media having a nonwoven first layer integral with a second layer containing a polyaeryionitrile (PAN) fibers, a filter aid, and a wet-strength resin. The depth filter media exhibits increased creased binding capacity for soluble impurities such as DNA and host cells proteins from biological/cell culture feed-streams during secondary clarification and low-level impurity clearance of harvested cell culture fluids, such as those used for the manufacture of monoclonal antibodies. The depth filter media additionally exhibits significantly lower flushing requirements, resulting in lower levels of organic, inorganic and bioburden extractables released, high dirt holding capacities and good chemical and/or radiation resistance.Type: ApplicationFiled: September 2, 2014Publication date: April 28, 2016Inventors: Maybelle Woo, David Yavorksy, John Amara, Nripen Singh
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Publication number: 20160067703Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.Type: ApplicationFiled: November 13, 2015Publication date: March 10, 2016Inventors: Jad Jaber, Wilson Moya, James Hamzik, Arezki Boudif, Yu Zhang, Neil Soice, John Charkoudian, Nripen Singh
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Patent number: 9217048Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.Type: GrantFiled: February 19, 2014Date of Patent: December 22, 2015Assignee: EMD Millipore CorporationInventors: Jad Jaber, Wilson Moya, James Hamzik, Arezki Boudif, Yu Zhang, Neil Soice, John Charkoudian, Nripen Singh
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Publication number: 20150133643Abstract: Provided is a primary clarification depth filtration process of cell-culture feeds, including chemically treated flocculated feeds, containing target biomolecules of interest such as mAbs, mammalian cell cultures, or bacterial cell cultures, utilizing a primary clarification depth filtration device containing a media with significantly lower flushing requirements, resulting in lower levels of organic extractables released after media flushing, and increased throughput for the pre-treated feed streams, without the use of a primary clarification centrifugation step or primary clarification tangential flow microfiltration step. The primary clarification depth filtration device used in the primary clarification of fluid cell culture feeds, including chemically treated flocculated feeds containing flocculated cellular debris and/or colloidal particulates having a particle size distribution of about 0.Type: ApplicationFiled: June 6, 2013Publication date: May 14, 2015Inventors: Kwok-Shun Cheng, Nripen Singh