Abstract: Disclosed is a microfluidic cartridge that can perform highly accurate lab quality DNA tests and other amplification based tests at the point of care. The cartridge may have slanted channels that angle downward from the inlet well, where the sample is deposited, to the reaction well, where the sample will flow down to and initiate the reaction. In some examples, the cartridge may be fabricated from specific materials that enhance passive flow through the channels. Additionally, the reaction wells have an access port that allows ventilation to the reaction wells that enhances the flow of the sample from the inlet to the reaction well. The access ports allow convenient access for depositing the reaction mixture into the reaction wells after the cartridge is fabricated.

Abstract: The inventors have developed a clinical decision support system (“CDSS”) and associated devices to diagnose chronic diseases in patients using a large number of biomarkers. The inventors have utilized a process for identifying thousands of biomarkers that could be relevant to potential diseases. Once these biomarkers are identified, the ones that have the most affinity for relevant biomarkers are retained. Then, a clinical decision support system can utilize the thousands of biomarkers, and apply a DNN based machine learning algorithm to diagnose chronic diseases.