Patents by Inventor Nurith Kurn

Nurith Kurn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190119746
    Abstract: Provided herein are methods and compositions for selective amplification of nucleic acids. The compositions include oligonucleotides with sequence features that allow simultaneous, parallel amplification of multiple targets from a mixture of nucleic acids in a single reaction. Methods of using such oligonucleotides to identify individual targets and create libraries of targets from mixtures of nucleic acids are also provided.
    Type: Application
    Filed: October 18, 2018
    Publication date: April 25, 2019
    Inventors: Doug Amorese, Benjamin G. Schroeder, Nurith Kurn, Ashesh Saraiya
  • Publication number: 20190078082
    Abstract: The present invention provides methods, compositions and kits for targeted nucleic acid sequence enrichment in a nucleic acid sample and for high efficiency nucleic acid library generation for next generation sequencing (NGS). Specifically, the methods, compositions and kits provided herein are useful for the production and capture of amplification-ready, target-specific and strand-specific regions of interest from nucleic acid samples containing complex DNA.
    Type: Application
    Filed: June 25, 2018
    Publication date: March 14, 2019
    Inventors: Doug AMORESE, Chris ARMOUR, Nurith KURN
  • Patent number: 10036012
    Abstract: The present invention provides methods, compositions and kits for targeted nucleic acid sequence enrichment in a nucleic acid sample and for high efficiency nucleic acid library generation for next generation sequencing (NGS). Specifically, the methods, compositions and kits provided herein are useful for the production and capture of amplification-ready, target-specific and strand-specific regions of interest from nucleic acid samples containing complex DNA.
    Type: Grant
    Filed: March 28, 2017
    Date of Patent: July 31, 2018
    Assignee: NUGEN TECHNOLOGIES, INC.
    Inventors: Doug Amorese, Chris Armour, Nurith Kurn
  • Patent number: 9957549
    Abstract: The present invention provides methods, compositions and kits for the generation of next generation sequencing (NGS) libraries in which non-desired nucleic acid sequences have been depleted or substantially reduced. The methods, compositions and kits provided herein are useful, for example, for the production of libraries from total RNA with reduced ribosomal RNA and for the reduction of common mRNA species in expression profiling from mixed samples where the mRNAs of interest are present at low levels. The methods of the invention can be employed for the elimination of non-desired nucleic acid sequences in a sequence-specific manner, and consequently, for the enrichment of nucleic acid sequences of interest in a nucleic acid library.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: May 1, 2018
    Assignee: NUGEN TECHNOLOGIES, INC.
    Inventors: Christopher Armour, Doug Amorese, Bin Li, Nurith Kurn
  • Publication number: 20170298345
    Abstract: The present invention provides methods, compositions and kits for targeted nucleic acid sequence enrichment in a nucleic acid sample and for high efficiency nucleic acid library generation for next generation sequencing (NGS). Specifically, the methods, compositions and kits provided herein are useful for the production and capture of amplification-ready, target-specific and strand-specific regions of interest from nucleic acid samples containing complex DNA.
    Type: Application
    Filed: March 28, 2017
    Publication date: October 19, 2017
    Inventors: Doug AMORESE, Chris ARMOUR, Nurith KURN
  • Patent number: 9650628
    Abstract: The present invention provides methods, compositions and kits for targeted nucleic acid sequence enrichment in a nucleic acid sample and for high efficiency nucleic acid library generation for next generation sequencing (NGS). Specifically, the methods, compositions and kits provided herein are useful for the production and capture of amplification-ready, target-specific and strand-specific regions of interest from nucleic acid samples containing complex DNA.
    Type: Grant
    Filed: January 25, 2013
    Date of Patent: May 16, 2017
    Assignee: NUGEN TECHNOLOGIES, INC.
    Inventors: Doug Amorese, Chris Armour, Nurith Kurn
  • Publication number: 20160362680
    Abstract: The present invention provides methods, compositions and kits for the generation of next generation sequencing (NGS) libraries in which non-desired polynucleotides have been depleted or substantially reduced. The methods, compositions and kits provided herein are useful, for example, for the production of libraries from total RNA with reduced ribosomal RNA and for the reduction of common mRNA species in expression profiling from mixed samples where the mRNAs of interest are present at low levels. The methods of the invention can be employed for the elimination of non-desired polynucleotides in a sequence-specific manner, and consequently, for the enrichment of nucleic acid sequences of interest in a nucleic acid library.
    Type: Application
    Filed: May 13, 2016
    Publication date: December 15, 2016
    Inventors: Christopher Armour, Doug Amorese, Bin Li, Nurith Kurn
  • Publication number: 20160153039
    Abstract: Provided herein are methods, compositions and kits for targeted nucleic acid sequence enrichment in a nucleic acid sample and for high efficiency nucleic acid library generation for next generation sequencing (NGS). The methods, compositions and kits provided herein can be useful for the production and capture of amplification-ready, target-specific and strand-specific regions of interest from nucleic acid samples containing complex DNA.
    Type: Application
    Filed: August 26, 2015
    Publication date: June 2, 2016
    Inventors: Doug Amorese, Nurith Kurn, Jonathan Scolnick, Alexandra Hui Wang
  • Patent number: 9181582
    Abstract: The invention provides methods for isothermal amplification of RNA. The methods are particularly suitable for amplifying a plurality of RNA species in a sample. The methods employ a composite primer, a second primer and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In another aspect, the methods employ a single primer (which is a composite primer) and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In some embodiments, a transcription step is included to generate multiple copies of sense RNA of an RNA sequence of interest. The methods are useful for preparation of nucleic acid libraries and substrates for analysis of gene expression of cells in biological samples. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
    Type: Grant
    Filed: June 19, 2013
    Date of Patent: November 10, 2015
    Assignee: NUGEN TECHNOLOGIES, INC.
    Inventor: Nurith Kurn
  • Patent number: 9175325
    Abstract: The invention relates to the field of polynucleotide amplification. More particularly, the invention provides methods, compositions and kits for amplification of (i.e., making multiple copies of) a multiplicity of different polynucleotide template sequences using a randomly primed RNA/DNA composite primer.
    Type: Grant
    Filed: June 14, 2013
    Date of Patent: November 3, 2015
    Assignee: NUGEN TECHNOLOGIES, INC.
    Inventors: Nurith Kurn, Shenglong Wang
  • Publication number: 20150299767
    Abstract: The present invention provides methods, compositions and kits for the generation of next generation sequencing (NGS) libraries in which non-desired nucleic acid sequences have been depleted or substantially reduced. The methods, compositions and kits provided herein are useful, for example, for the production of libraries from total RNA with reduced ribosomal RNA and for the reduction of common mRNA species in expression profiling from mixed samples where the mRNAs of interest are present at low levels. The methods of the invention can be employed for the elimination of non-desired nucleic acid sequences in a sequence-specific manner, and consequently, for the enrichment of nucleic acid sequences of interest in a nucleic acid library.
    Type: Application
    Filed: March 15, 2013
    Publication date: October 22, 2015
    Inventors: Christopher Armour, Doug Amorese, Bin Li, Nurith Kurn
  • Patent number: 8852867
    Abstract: The invention provides methods for amplification of polynucleotide sequences using primers containing single-stranded RNA. The methods employ use of an enzyme capable of cleaving single-stranded RNA, such as RNase I, to degrade a first RNA-containing primer prior to addition of a second RNA-containing primer. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
    Type: Grant
    Filed: May 9, 2011
    Date of Patent: October 7, 2014
    Assignee: Nugen Technologies, Inc.
    Inventors: Nurith Kurn, Shenglong Wang
  • Publication number: 20140274729
    Abstract: The invention provides methods and compositions, including kits, for the construction of directional nucleic acid libraries. The invention further provides methods and compositions for the amplification and sequencing of directional cDNA libraries.
    Type: Application
    Filed: September 18, 2013
    Publication date: September 18, 2014
    Applicant: NuGEN Technologies, Inc.
    Inventors: Nurith Kurn, Bin Li
  • Publication number: 20140065692
    Abstract: The invention provides methods, compositions, and kits for fragmentation and labeling of nucleic acids. More particularly, the invention relates to methods for fragmentation of nucleic acids to produce fragments with 3? end hydroxyl groups within a desired size range. In methods of the invention, nucleic acids are fragmented at abasic sites to produce fragments with blocked 3? ends. The 3? ends are unblocked to produce polynucleotide fragments with hydroxyl groups at their 3? ends. Methods, kits, and compositions for carrying out fragmentation of a polynucleotide template in a single reaction mixture to yield fragments with 3?-hydroxl ends within the desired size range are disclosed.
    Type: Application
    Filed: August 28, 2013
    Publication date: March 6, 2014
    Inventors: Nurith KURN, Pengchin Chen
  • Publication number: 20140038188
    Abstract: The invention provides methods for isothermal amplification of RNA. The methods are particularly suitable for amplifying a plurality of RNA species in a sample. The methods employ a composite primer, a second primer and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In another aspect, the methods employ a single primer (which is a composite primer) and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In some embodiments, a transcription step is included to generate multiple copies of sense RNA of an RNA sequence of interest. The methods are useful for preparation of nucleic acid libraries and substrates for analysis of gene expression of cells in biological samples. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
    Type: Application
    Filed: June 19, 2013
    Publication date: February 6, 2014
    Inventor: Nurith Kurn
  • Publication number: 20140038236
    Abstract: The invention relates to the field of polynucleotide amplification. More particularly, the invention provides methods, compositions and kits for amplification of (i.e., making multiple copies of) a multiplicity of different polynucleotide template sequences using a randomly primed RNA/DNA composite primer.
    Type: Application
    Filed: June 14, 2013
    Publication date: February 6, 2014
    Inventors: Nurith Kurn, Shenglong Wang
  • Patent number: 8551709
    Abstract: The invention provides methods, compositions, and kits for fragmentation and labeling of nucleic acids. More particularly, the invention relates to methods for fragmentation of nucleic acids to produce fragments with 3? end hydroxyl groups within a desired size range. In methods of the invention, nucleic acids are fragmented at abasic sites to produce fragments with blocked 3? ends. The 3? ends are unblocked to produce polynucleotide fragments with hydroxyl groups at their 3? ends. Methods, kits, and compositions for carrying out fragmentation of a polynucleotide template in a single reaction mixture to yield fragments with 3?-hydroxyl ends within the desired size range are disclosed.
    Type: Grant
    Filed: March 2, 2012
    Date of Patent: October 8, 2013
    Assignee: NuGEN Technologies, Inc.
    Inventors: Nurith Kurn, Pengchin Chen
  • Publication number: 20130231253
    Abstract: The present invention provides methods, compositions and kits for targeted nucleic acid sequence enrichment in a nucleic acid sample and for high efficiency nucleic acid library generation for next generation sequencing (NGS). Specifically, the methods, compositions and kits provided herein are useful for the production and capture of amplification-ready, target-specific and strand-specific regions of interest from nucleic acid samples containing complex DNA.
    Type: Application
    Filed: January 25, 2013
    Publication date: September 5, 2013
    Inventors: Doug Amorese, Chris Armour, Nurith Kurn
  • Patent number: 8512956
    Abstract: The present method provides methods, libraries, and kits related to the archiving and clonal amplification of sequences related to target polynucleotide sequences. The method allows for the generation and attachment of polynucleotides with defined 3? and 5? ends to solid surfaces. The polynucleotides attached to the solid substrates can be stored or archived as libraries and can subsequently be retrieved for analysis, for example by clonal amplification using a single composite amplification primer comprising a DNA portion and an RNA portion. In some embodiments, nucleotides attached to solid surfaces can be used for sequencing of nucleotide sequences related to the target DNA. The methods are applicable to total RNA and/or total DNA analysis.
    Type: Grant
    Filed: August 9, 2011
    Date of Patent: August 20, 2013
    Assignee: Nugen Technologies, Inc.
    Inventor: Nurith Kurn
  • Patent number: 8492095
    Abstract: The invention provides methods for isothermal amplification of RNA. The methods are particularly suitable for amplifying a plurality of RNA species in a sample. The methods employ a composite primer, a second primer and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In another aspect, the methods employ a single primer (which is a composite primer) and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In some embodiments, a transcription step is included to generate multiple copies of sense RNA of an RNA sequence of interest. The methods are useful for preparation of nucleic acid libraries and substrates for analysis of gene expression of cells in biological samples. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
    Type: Grant
    Filed: October 27, 2011
    Date of Patent: July 23, 2013
    Assignee: Nugen Technologies, Inc.
    Inventor: Nurith Kurn