Abstract: Provided by this disclosure is a method and system for the preparation of a blood clot-based wound dressing whereby a fluid, typically gas such as air is propelled through one conduit into a sealed, blood-containing test tube, and at the same time blood s permitted to flow out of the test through a second conduit and thereby transferred into a blot clot molding space where the blood is permitted to coagulate forming a clot of a shape defined by the molding space. The propelling fluid is typically air that is propelled to flow in the first conduit by a syringe. Provide by this disclosure is also a coupling device useful in said method and system; as well as a kit of parts comprising elements needed for carrying out the method of this disclosure.