Abstract: MHC multimers are provided in which peptide-loaded MHC monomers are covalently linked to a multimerization domain through conjugation moieties on the monomers and the multimerization domain. The multimers can further comprise oligonucleotide barcodes. Peptide exchange can be performed with a plurality of pMHC multimers to create pMHC multimer libraries. Methods of making and using the pMHC multimers and libraries are also provided. Peptide-loaded MHC Class I and MHC Class II multimers, and libraries thereof, are provided.

Abstract: Described herein are compositions of hydrogels and methods of use thereof.

Abstract: The disclosure provides a peptide library with increased peptide diversity. The increase in peptide diversity can occur via cleavage of particular amino acids within a peptide. The disclosure further provides a method for promoting folding of a peptide into an active conformation.