Patents by Inventor Oleh Petriv
Oleh Petriv has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220365072Abstract: Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.Type: ApplicationFiled: July 7, 2021Publication date: November 17, 2022Applicant: The University of British ColumbiaInventors: Marketa RICICOVA, Kevin Albert HEYRIES, Hans ZAHN, Oleh PETRIV, Veronique LECAULT, Anupam Singhal, Daniel J. Da Costa, Carl L. G. Hansen, Brad NELSON, Julie NIELSEN, Kathleen Lisaingo
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Publication number: 20200363401Abstract: Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.Type: ApplicationFiled: July 16, 2020Publication date: November 19, 2020Applicant: The University of British ColumbiaInventors: Marketa RICICOVA, Kevin Albert HEYRIES, Hans ZAHN, Oleh PETRIV, Veronique LECAULT, Anupam Singhal, Daniel J. Da Costa, Carl L. G. Hansen, Brad NELSON, Julie NIELSEN, Kathleen Lisaingo
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Patent number: 10725024Abstract: Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.Type: GrantFiled: March 28, 2014Date of Patent: July 28, 2020Assignee: THE UNIVERSITY OF BRITISH COLUMBIAInventors: Marketa Ricicova, Kevin Albert Heyries, Hans Zahn, Oleh Petriv, Veronique Lecault, Anupam Singhal, Daniel J. Da Costa, Carl L. G. Hansen, Brad Nelson, Julie Nielsen, Kathleen Lisaingo
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Patent number: 10508299Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.Type: GrantFiled: October 25, 2017Date of Patent: December 17, 2019Assignee: The University of British ColumbiaInventors: Carl Lars Genghis Hansen, Michael Vanlnsberghe, Adam White, Oleh Petriv, Tim Leaver, Anupam Singhal, William Bowden, Veronique Lecault, Dan Da Costa, Leo Wu, Georgia Russell, Darek Sikorski
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Publication number: 20180230512Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.Type: ApplicationFiled: October 25, 2017Publication date: August 16, 2018Inventors: Carl Lars Genghis Hansen, Michael VanInsberghe, Adam White, Oleh Petriv, Tim Leaver, Anupam Singhal, William Bowden, Veronique Lecault, Dan Da Costa, Leo Wu, Georgia Russell, Darek Sikorski
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Patent number: 9902990Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.Type: GrantFiled: May 27, 2011Date of Patent: February 27, 2018Assignee: The University of British ColumbiaInventors: Carl Lars Genghis Hansen, Michael VanInsberghe, Adam White, Oleh Petriv, Tim Leaver, Anupam Singhal, William Bowden, Veronique Lecault, Dan Da Costa, Leo Wu, Georgia Russell, Darek Sikorski
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Publication number: 20160252495Abstract: Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.Type: ApplicationFiled: March 28, 2014Publication date: September 1, 2016Inventors: Marketa Ricicova, Kevin Albert Heyries, Hans Zahn, Oleh Petriv, Veronique Lecault, Anupam Singhal, Daniel J. Da Costa, Carl L. G. Hansen, Brad Nelson, Julie Nielsen, Kathleen Lisaingo
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Publication number: 20150018226Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.Type: ApplicationFiled: May 27, 2011Publication date: January 15, 2015Inventors: Carl Lars Genoble Hansen, Michael Vaninsberghe, Adam White, Oleh Petriv, Tim Leaver, Anupam Singhal, William Bowden, Veronique Lecault, Dan Da Costa, Leo Wu, Georgia Russell, Darek Sikorski
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Publication number: 20130022973Abstract: Kits, primers, and methods are provided herein for detecting relative target source to reference source ratios in a biological sample, by distributing the biological sample into discrete subsamples, wherein the biological sample includes, a plurality of target molecules on a target source; and a plurality of reference molecules on a reference source; providing target primers directed to one or more of the plurality of target molecules and reference primers directed to one or more of the plurality of reference molecules; performing digital amplification with the target primers and the reference primers; and detecting the presence or absence of amplified target products with target probes and detecting the presence or absence of amplified reference products with reference probes, wherein the ratio of amplified target products to amplified reference products is indicative of a relative amount of target source to reference source in a biological sample.Type: ApplicationFiled: January 14, 2011Publication date: January 24, 2013Inventors: Carl L. G. Hansen, Oleh Petriv, Kevin Heyries, Kenneth J. Livak