Abstract: A system for time-resolved fluoroimmunoassay detection is disclosed. The system comprises a receptacle with a sample volume adapted for receiving a sample therein; a light source adapted to emit pulsed excitation light illumination optics adapted to collect the pulsed excitation light from the light source and to deliver the pulsed excitation light to the sample volume in the receptacle; a detection device adapted for gated detection of fluorescence radiation at least in a detection spectral range; detection optics adapted to collect fluorescence radiation from the receptacle at least in the detection spectral range and deliver the fluorescence radiation to the detection device; and an optical filter device configured for the separation of excitation light and detection light. A method for time-resolved fluoroimmunoassay detection is also provided.

Abstract: A system (100) for time-resolved fluoroimmunoassay detection is disclosed. The system (100) comprises a receptacle (110) with a sample volume adapted for receiving a sample (99) therein; a light source (120) adapted to emit pulsed excitation light (200); illumination optics (130) adapted to collect the pulsed excitation light (200) from the light source (120) and to deliver said pulsed excitation light (200) to the sample volume in the receptacle (110); a detection device (140) adapted for gated detection of fluorescence radiation (210) at least in a detection spectral range; detection optics (150) adapted to collect fluorescence radiation (210) from the receptacle (110) at least in the detection spectral range and deliver said fluorescence radiation (210) to the detection device (140); and an optical filter device (160) configured for the separation of excitation light (200) and detection light (210).