Abstract: Disclosed herein is a method for detecting cell aggregates of biological cells using a quantitative phase-contrast microscope, a device for detecting cell aggregates of biological cells, a method for detecting cellular and/or molecular biological objects using a quantitative phase-contrast microscope and a device for detecting cellular and/or molecular biological objects. The method for detecting cell aggregates of biological cells using a quantitative phase-contrast microscope comprises preparing a suspension, the suspension comprising a viscoelastic fluid and biological cells from a sample, wherein the viscoelastic fluid comprises a shear-thinning polymer having a molecular weight between 2 MDa and 10 MDa and wherein a mass fraction of the shear-thinning polymer in the suspension is less than 0.2%. A flow of the suspension is generated along a microfluidic channel to viscoelastically focus cell aggregates in the suspension in a focal plane of the quantitative phase-contrast microscope.

Abstract: An in-vitro method for determining a cell type of a white blood cell in a biological sample does so without labeling, wherein a microscopy apparatus images the cell, and physical parameters of the cell are ascertained from the image of the cell by an automated image analysis. The cell type of the white blood cell is determined on the basis of the physical parameters and on the basis of principal component analysis parameters (PCA parameters) , wherein the principal component analysis parameters comprise linear combinations of at least some of the physical parameters.

Abstract: Disclosed herein is a method for detecting cell aggregates of biological cells using a quantitative phase-contrast microscope and a device for detecting cell aggregates of biological cells using said method. The method comprises preparing a suspension comprising biological cells from a sample. A flow of the suspension is generated along a microfluidic channel to viscoelastically and/or hydrodynamically focus cell aggregates in the suspension in a focal plane of the quantitative phase-contrast microscope. One or more phase shift images of the biological cells in the suspension are taken using the quantitative phase-contrast microscope. Cell aggregates in the one or more phase shift images are identified. The sample is a whole blood sample or a blood fraction sample and identifying cell aggregates in the one or more phase shift images comprises identifying platelet aggregates in the one or more phase shift images.

Abstract: The invention refers to a testing device (to) for testing the susceptibility of a biological probe to one or more bioactive substances. The testing device comprises a liquid-impermeable first substrate (12), a second substrate (14) arranged or arrangeable over the first substrate (12), wherein the second substrate (14) is liquid-permeable and liquid-impregnable, and a third substrate (16) arranged or arrangeable over the second substrate (14), wherein the third substrate (16) is liquid-impermeable. The first substrate and/or the third substrate are configured for encapsulating the second substrate. At least a portion of the second substrate (14) is exposable to an environment of the testing device for receiving the biological probe. The invention further refers to a testing kit comprising such a testing device and at least one of one or more bioactive substances, a biological culture medium, and a bacterial marking substance configured for undergoing a property change in the presence of bacteria.

Abstract: Disclosed herein is a method of analyzing a tissue sample using a quantitative phase-contrast microscope as well as a corresponding microfluidic system and a corresponding device. The method comprises providing the tissue sample in a sample volume of a microfluidic system, wherein the tissue sample comprises a plurality of biological cells forming a continuous tissue material. At least a part of the tissue sample is dissolved into single cells and/or cell aggregates in a carrier fluid in the sample volume. A flow of the carrier fluid is generated from the sample volume to a measurement volume of the microfluidic system and a first phase shift image of the single cells and/or cell aggregates in the measurement volume is taken with the quantitative phase-contrast microscope.

Abstract: The invention relates to a method for sensing target molecules in an analyte solution, a sensor for sensing target molecules in an analyte solution and a measurement system for sensing target molecules in an analyte solution. The method comprises providing a capture surface, wherein a plurality of capture molecules are arranged on the capture surface, each of the capture molecules being configured to bind to at least one of said target molecules. The method further comprises exposing the capture surface to the analyte solution to allow target molecules to bind to the capture molecules arranged on the capture surface.

Abstract: Disclosed herein is an endoprosthetic spacer (100, 200, 300) for administering a therapeutic treatment, in particular a theragnostic treatment. The endoprosthetic spacer (100, 200, 300) comprises a body (102) that is configured to replace at least a part of a bone, a sensor assembly (104) comprising at least one sensor (104A, 104B, 104C), a communication module (108) configured to transmit a signal; and a controller (106) configured to read out a sensor signal from the at least one sensor (104A, 104B, 104C) and to transmit an output signal (110) via the communication module (108).

Abstract: Disclosed herein is a device (100) for multicolor optical imaging of a sample (102) with wavelength-dependent optical path length enhancement, the device (100) comprising an optical resonator (106) for enhancing an optical path length, wherein the optical resonator (106) has a first finesse at a first wavelength and a second finesse at a second wavelength; a sample holder (104) for mounting the sample (102) in the optical resonator (106), wherein the sample holder (104) is configured to hold the sample (102) such that an optical axis (112) of the optical resonator (106) intersects with the sample (102);a first imaging system (114) for imaging the sample (102) at the first wavelength with a first imaging technique, and a second imaging system (126) for imaging the sample (102) at the second wavelength with a second imaging technique, wherein the second wavelength is different from the first wavelength; wherein the first finesse and the second finesse are chosen such that the optical resonator (106) enhances a

Abstract: The invention relates to a device, such as a digital holographic microscope, for detecting and processing a first full image of a measurement object, measured with a first offset, wherein an arrangement is provided for generating at least one further full image with at least one offset that differs from the first offset.

Abstract: Disclosed herein is a device (100) for multicolor optical imaging of a sample (102) with wavelength-dependent optical path length enhancement, the device (100) comprising an optical resonator (106) for enhancing an optical path length, wherein the optical resonator (106) has a first finesse at a first wavelength and a second finesse at a second wavelength; a sample holder (104) for mounting the sample (102) in the optical resonator (106), wherein the sample holder (104) is configured to hold the sample (102) such that an optical axis (112) of the optical resonator (106) intersects with the sample (102);a first imaging system (114) for imaging the sample (102) at the first wavelength with a first imaging technique, and a second imaging system (126) for imaging the sample (102) at the second wavelength with a second imaging technique, wherein the second wavelength is different from the first wavelength; wherein the first finesse and the second finesse are chosen such that the optical resonator (106) enhances a

Abstract: A method for aggregating a cluster of particles having a target cluster constitution in a channel comprising a retention section comprises the steps of establishing a fluid stream comprising a fluid carrier medium and particles of at least one type through said channel; controlling a supply of said particles of at least one type into the fluid stream; operating a retention mechanism to aggregate at least part of said particles in an aggregation region within said retention section, to thereby form said cluster of particles; monitoring, while operating said retention mechanism, the particles in at least part of said channel for obtaining a monitoring signal associated with the cluster and/or with the particles moving in the fluid stream; determining a current cluster constitution from the monitoring signal; comparing said current cluster constitution with said target cluster constitution; and controlling at least one of said particle retention mechanism and said supply of said particles of at least one type, s

Abstract: The invention relates to an in-vitro method for determining a cell type of a white blood cell in a biological sample without labeling, wherein a microscopy apparatus images the cell and physical parameters of the cell are ascertained from the image of the cell by means of an automated image analysis, wherein the cell type of the white blood cell is determined on the basis of the physical parameters and on the basis of principal component analysis parameters (PCA parameters), wherein the principal component analysis parameters comprise linear combinations of at least some of the physical parameters.

Abstract: The present invention relates to a method of detecting a possible infection of malaria in a patient using a digital optical microscope.

Abstract: A computer-implemented method for analyzing digital holographic microscopy (DHM) data for hematology applications includes receiving a DHM image acquired using a digital holographic microscopy system. The DHM image comprises depictions of one or more cell objects and background. A reference image is generated based on the DHM image. This reference image may then be used to reconstruct a fringe pattern in the DHM image into an optical depth map.

Abstract: The invention relates to a method and a corresponding arrangement for sequencing at least two biopolymers (6), wherein for each biopolymer (6) a sequence signal is picked up by a respective measured variable pickup on the basis of the sequence of the biopolymer (6), the sequence signals are transferred to a shift register (16) and buffer-stored therein, the buffer-stored sequence signals are transferred from the shift register (16) sequentially to an evaluation device (26) and evaluated therein. Each sequence signal is preferably produced here by means of a nanopore arrangement (10). A corresponding sequencing arrangement (11) has the measured variable pickups and the shift register (16) integrated in it, preferably in an electrical circuit, that is to say on a sensor array, for example. Each sequence signal can be amplified here by a preamplifier (14) prior to transfer to the shift register (16).

Abstract: The invention relates to a device for examining particles in a liquid to be examined, comprising a flow passage through which the liquid to be examined is moved. The flow passage has at least one inlet through which at least one sheath fluid flows into the flow passage such that the at least one sheath fluid forms at least one sheath flow in the flow passage. The device further comprises a wave generating device for piezoacoustically generating sound waves which propagate through the flow passage transversely to the flow direction of the liquid to be examined and form wave nodes on a monitoring plane such that particles to be examined of the liquid to be examined are moved onto the monitoring plane and accumulate thereon on the basis of the pressure effect of the sound waves in the transverse direction.

Abstract: The present invention relates to an improved method for marker-free detection of a cell type of at least one cell in a medium using microfluidics and digital holographic microscopy, as well as a device, particular for carrying out the method.

Abstract: The present invention relates to a method of detecting a possible infection of malaria in a patient using a digital optical microscope

Abstract: A flexible, thin, elongated band is used as a substrate. Similarly to a magnetic tape, the band is unwound from a feed reel and is transported past an outlet opening of a receptacle containing the cells such that the cells are poured onto the band. Subsequently, the band containing the cells applied thereon is wound onto a take-up reel. The take-up reel is fixed on a drive shaft which can be rotated by a drive mechanism. The rotation thus achieved has the effect that the band is unwound, transported past the outlet opening and finally wound up. In addition, spacers are provided at the upper surface of the band in order to prevent the contacting of radially adjacent sections of the band containing the cells in a wound-up state.

Abstract: A technique is presented for aligning, in a desired region within a flow chamber of a flow cell, a non-spherical biological entity carried in a sample. The flow chamber has a rectangular cross-section. A bottom flow input module, a top flow input module and a sample input module provide a viscoelastic first fluid, a second viscoelastic fluid, and the sample, respectively, to the flow chamber. The first and the second viscoelastic fluids laminarly flow along a bottom and a top wall of the flow chamber and the sample laminarly flows sandwiched between them. By controlling rate of flow of the first and/or the second viscoelastic fluids the sample flow, and thus the non-spherical biological entity, is focused in the desired region. A gradient of sheer within the sample flow set up due to the first and second viscoelastic fluids orients the non-spherical biological entity in the desired region.