Abstract: This disclosure relates to novel mjresynthesis kits and methods, in particular for use in pairwise sequencing.

Abstract: An optical device and systems for near and distance imaging includes an optical unit with a first optical lens and a second optical lens, the first optical lens and second optical lens being arranged along an optical axis, and a transceiver unit with a transceiver element, a light passage surface of the transceiver element being arranged in or intersecting a plane or curved surface. A first distance between the surface and the first optical lens is provided along the optical axis and set in such a way that, during intended normal operation, the transceiver element of the transceiver unit is imaged onto a distant imaging surface by the optical unit.

Abstract: Embodiments of the present disclosure relate to modified extension primers for use in generating clustered polynucleotides for sequencing by synthesis. In particular, the disclosure relates to methods of chemically linearizing clustered polynucleotides in preparation for sequencing by cleavage of one or more strands of double-stranded polynucleotides immobilized on a solid support by a periodate salt.

Abstract: The present disclosure is concerned with compositions and methods for reducing the steps used in the generation of monoclonal clusters by combining the enzymes used for linearization and removal of unused surface primers.

Abstract: The present disclosure is concerned with compositions and methods for reducing the steps used in the generation of monoclonal clusters by combining the enzymes used for linearization and removal of unused surface primers.

Abstract: The present disclosure relates to a composition including one or more modified nucleotide, wherein the modified nucleotide comprises a purine or pyrimidine base and a sugar moiety having a 3?-hydroxy blocking group, and a radical scavenger, wherein the composition is lyophilised. The present disclosure further relates to a composition including one or more functional protein; one or more functional protein activator; and one or more non-reducing sugar, wherein the composition is lyophilised. Also disclosed are methods of rehydration of one or more compositions described herein and kits including one or more compositions described herein. Further disclosed are cartridges including a flow cell comprising one or more reagent reservoirs, where the one or more reagent reservoirs include one or more compositions described herein.

Abstract: A method for purifying nucleotides is provided, that includes preparing a solution comprising (a) 3?-blocked nucleotides, (b) 3?-OH nucleotides, (c) a polishing polymerase, and (d) a template. The polishing polymerase and the template are used to selectively polymerize the 3?-OH nucleotides and thus reduce a concentration in the solution of the 3?-OH nucleotides relative to the 3?-blocked nucleotides.

Abstract: The present disclosure is concerned with compositions and methods for reducing the steps used in the generation of monoclonal clusters by combining the enzymes used for linearization and removal of unused surface primers.

Abstract: The invention relates to the rapid enrichment of nucleic acid molecules of interest from complex mixtures of nucleic acids for the purpose of sequencing genes and variants, e.g. for clinical uses as well as other applications. A hybridisation column comprising an inner channel, wherein a portion of said channel is filled completely with a porous solid support comprising (a) a plurality of interconnected, micron-sized voids that permit a fluid to flow between them and the remainder of the channel, and (b) a plurality of hybridisation probes, which are bound to the surfaces of the solid support forming the voids is disclosed.

Abstract: A method for the measurement of the amount or difference in the amounts of 2 or more nucleic acid targets in a sample, the method comprising the steps of attaching to nucleic acids present in the sample (1) a tag which allows the nucleic acids to be captured to a solid support; and (2) a labelled probe for a first nucleic acid target present in the sample and a labelled probe for second nucleic acid target present in the sample, and then measuring the amount of each labelled probe or difference in the amount of labelled probes; wherein the probe is not a single labelled nucleotide.