Abstract: The disclosure relates to methods for handling and supplementation of cell culture medium to improve process performance in eukaryotic recombinant expression systems.

Abstract: The current invention reports the use of meta-tyrosine for increasing the specific productivity of a eukaryotic cell that produces/expresses a polypeptide. In the current method it is not necessary to perform a temperature-, osmolality- or pH shift or to add drugs like valproic acid or sodium butyrate to modulate the specific productivity of the cultivated cells. The method does not affect cell viability or product titer.

Abstract: Disclosed is a tube configured to conduct a fluid flowing through the tube in a specified flow direction and for this purpose comprises a tube wall, which encloses a lumen of the tube, and an interference body, which is arranged within the tube but is nevertheless connected to the tube wall at an inner face of the tube wall facing the lumen. In the tube according to the present disclosure, the tube wall has a maximum wall thickness of more than 1 mm and at least two mutually spaced sub-segments with a respective wall thickness that deviates from said maximum wall thickness, wherein the sub-segment is positioned upstream of the interference body in the flow direction, and the sub-segment is positioned downstream of the sub-segment in the flow direction.

Abstract: Herein is reported a method for determining if process data acquired during the cultivation of a mammalian or bacterial cell is affected by a problem comprising the steps of (i) fitting the process data acquired during the cultivation of a mammalian or bacterial cell clone expressing a recombinant, heterologous polypeptide in a metabolic model generated for the same mammalian or bacterial cell expressing the same recombinant, heterologous polypeptide, and (ii) determining that the cultivation is affected by a problem if the modeled fit shows an offset with respect to the raw data of more than 10%, or the modeled fit has a chi2 value determined by a Pearson's chi-squared test of more than 5.

Abstract: A switchable cam lever that includes an inner lever and an outer lever is provided for a valve drive of an internal combustion engine. A transverse coupling serves for connecting the outer and inner lever to one another. A cam return spring is braced between the outer and inner lever. The return spring is installed upright, wherein the coupling runs directly above a support surface, and the return spring, as viewed in a lever longitudinal direction is guided in a receptacle in the inner lever. The receptacle is situated directly and entirely in front of the support surface and is formed as a bore, and the return spring bears at one end against a base or annular collar of the bore and acts at an opposite end against a crossbar which connects two arms of the outer lever at the top side.

Abstract: The present invention relates to methods for selecting between conditions which enhance cell growth or biomass generation and conditions which affect the N-glycosylation maturity of expressed glycoprotein produced by eukaryotic cells under fermentation culture conditions. Thus, in the methods of the present invention, the glycoprotein producing cells are cultured in a medium which is tailored to a desired end result. The invention also embraces the medium and the use thereof.

Abstract: The present disclosure relates to a method for producing a probe of a thermal flow meter for measuring the mass flow rate of a medium in a measuring tube, the method having the following steps: introducing a probe core in the form of a material to be melted into a first probe casing, the first probe casing having an open first end and a closed second end facing away from the first end; melting the probe core; quenching the probe core to a temperature below the solidification temperature; attaching a thermoelement to a contact surface of the solidified probe core. The invention also relates to a probe obtained according to the production method and to a flow meter including the probes according to the present disclosure.

Abstract: A switchable cam lever that includes an inner lever and an outer lever is provided for a valve drive of an internal combustion engine. A transverse coupling serves for connecting the outer and inner lever to one another. A cam return spring is braced between the outer and inner lever. The return spring is installed upright, wherein the coupling runs directly above a support surface, and the return spring, as viewed in a lever longitudinal direction is guided in a receptacle in the inner lever. The receptacle is situated directly and entirely in front of the support surface and is formed as a bore, and the return spring bears at one end against a base or annular collar of the bore and acts at an opposite end against a crossbar which connects two arms of the outer lever at the top side.

Abstract: The present disclosure relates to a method for manufacturing a probe of a thermal, flow measuring device for measuring mass flow of a liquid in a measuring tube, wherein the method includes: introducing a probe core including a hard solder and a core element into a first probe sleeve, wherein the first probe sleeve has an open first end and a closed second end away from the first end; melting the hard solder; affixing the core element by cooling the hard solder to a temperature less than the solidification temperature; and applying a thermoelement to a contact area of the core element or of the solidified hard solder. The present disclosure relates, furthermore, to a probe resulting from the manufacturing process as well as to a flow measuring device having at least one probe of the-present disclosure.

Abstract: The tube is used to conduct a fluid flowing through the tube in a specified flow direction and for this purpose comprises a tube wall (110), which encloses a lumen (100*) of the tube, and an interference body (120), which is arranged within the tube but is nevertheless connected to the tube wall at an inner face of the tube wall facing the lumen. In the tube according to the invention, the tube wall has a maximum wall thickness (smax) of more than 1 mm and at least two mutually spaced sub-segments (100-1, 100-2) with a respective wall thickness (s110-1, s110-2) that deviates from said maximum wall thickness (smax), wherein the sub-segment (100-1) is positioned upstream of the interference body (120) in the flow direction, and the sub-segment (100-2) is positioned downstream of the sub-segment (100-1) in the flow direction.

Abstract: The present disclosure relates to a method for producing a probe of a thermal flow meter for measuring the mass flow rate of a medium in a measuring tube, the method having the following steps: introducing a probe core in the form of a material to be melted into a first probe casing, the first probe casing having an open first end and a closed second end facing away from the first end; melting the probe core; quenching the probe core to a temperature below the solidification temperature; attaching a thermoelement to a contact surface of the solidified probe core. The invention also relates to a probe obtained according to the production method and to a flow meter including the probes according to the present disclosure.

Abstract: A measuring arrangement for determining flow velocity of at least one liquid phase and/or a gas phase of a vapor or a fluid composed of a liquid and a gaseous phase or a supercritical fluid, comprising a measuring tube, on or in which at least one sensor element of at least a first flow measuring device is arranged for measuring the liquid phase or the gas phase, wherein the measuring tube has at least an inflow region and an outflow region, wherein between these two regions a central region is arranged, whose measuring tube cross section has a greater area than the area of the measuring tube cross section of the outflow region or of the inflow region, and method for ascertaining flow of phases of a vapor or of a fluid composed of a liquid and a gaseous phase, or a supercritical fluid.

Abstract: A measuring device which has at least a first component, in which an integral measurement duct is provided or the first component forms in connection with additional components a measurement duct integrally in the measuring device. The measurement duct is provided for conducting a measured medium through the measuring device, characterized in that the first component has a first sensor for determining a first thermophysical property selected from thermal conductivity, thermal diffusivity and/or specific heat capacity of the measured medium, and wherein the measuring device has a second sensor, which vibrates and is provided for determining viscosity and/or density of the measured medium. The measured medium is conducted through the measurement duct from the first sensor to the second sensor.

Abstract: An apparatus for early warning against condensation induced hydraulic shock in a pipe, conveying a fluid present at least in a first part in the vapor phase and, in the case of condensate formation, in a second part in the form of a condensate, comprising at least two temperature sensors, which are arranged in the pipe and, thus, are fluid contacting, and at least one electronics unit, wherein the first temperature sensor is so arranged that it measures the temperature of the vapor, wherein the second temperature sensor is so arranged that it measures the temperature of the condensate, wherein the at least one electronics unit is so embodied that it ascertains the temperature difference between the temperature of the vapor and the temperature of the condensate, and, upon reaching a predetermined limit value for the temperature difference, generates a report.

Abstract: The current invention reports the use of meta-tyrosine for increasing the specific productivity of a eukaryotic cell that produces/expresses a polypeptide. In the current method it is not necessary to perform a temperature-, osmolality- or pH shift or to add drugs like valproic acid or sodium butyrate to modulate the specific productivity of the cultivated cells. The method does not affect cell viability or product titer.

Abstract: Herein is reported a method for producing an immunoconjugate with reduced product- and process-related impurities by culturing mammalian cells that contain one or more nucleic acids encoding the immunoconjugate of interest in a cell culture medium, wherein the one or more nucleic acids are expressed under the conditions of cell culture comprising the steps of: culturing the mammalian cells in a cell culture medium at a first temperature and at a first pH; reducing the first temperature of the cell culture medium to a second temperature; and increasing the first pH of the cell culture medium to a second pH; recovering the immunoconjugate from the cells or the cell culture medium, and thereby producing the immunoconjugate.

Abstract: A magneto-inductive flow measuring device comprising a measuring tube on which a magnet system and two or more measuring electrodes are arranged and/or secured. The measuring tube has in- and outlet regions with a first cross section and the measuring tube has between the in- and outlet regions a middle segment, which has a second cross section. The measuring electrodes are arranged in the middle segment of the measuring tube and the middle segment at least in the region of the measuring electrodes is surrounded by a tube holder, which guards against cross-sectional deformation of the second cross section.

Abstract: The invention relates to a method for manufacturing a probe (10) of a thermal, flow measuring device for measuring mass flow of a liquid in a measuring tube, wherein the method comprises steps as follows: introducing a probe core comprising a hard solder and a core element into a first probe sleeve, wherein the first probe sleeve has an open first end and a closed second end away from the first end; melting the hard solder; affixing the core element by cooling the hard solder to a temperature less than the solidification temperature; applying a thermoelement to a contact area of the core element or of the solidified hard solder. The invention relates, furthermore, to a probe resulting from the manufacturing process as well as to a flow measuring device having at least one probe of the invention.

Abstract: The current invention reports the use of meta-tyrosine for increasing the specific productivity of a eukaryotic cell that produces/expresses a polypeptide. In the current method it is not necessary to perform a temperature-, osmolality- or pH shift or to add drugs like valproic acid or sodium butyrate to modulate the specific productivity of the cultivated cells. The method does not affect cell viability or product titer.

Abstract: Provided herein are cell culture media and methods culturing host cells expressing polypeptides to reduce the level of trisulfide bonds in polypeptides produced by the host cells.