Abstract: The present invention relates to methods and transformed host cells for the production of eriodictyol from naringenin via bioconversion.

Abstract: The present invention relates to novel steviol glycosides R6-1, R6-2A, R6-2B, R6-4A, R6-4B and R7-2 and the production of these novel steviol glycosides, such as through enzymatic bioconversion. The use of these novel steviol glycosides as sweeteners and in orally consumable products are also provided.

Abstract: The present disclosure describes a method for replacing a photoresist (PR) bottle using a vehicle. An exemplary vehicle includes a processor configured to receive a request signal to replace a first PR bottle. The processor is also configured to transmit an order based on the request signal. The vehicle also includes a plurality of wheels configured to move the vehicle from the first location to a second location, and from the second location to the first location. The vehicle further includes a robotic arm configured to load, at the first location, the first PR bottle into a first container; load a second PR bottle in a second container; remove a cap from the second PR bottle and a socket from the first PR bottle; couple the socket of the first PR bottle to the second PR bottle; and unload the second PR bottle from the second container.

Abstract: The present disclosure describes a method for replacing a photoresist (PR) bottle using a vehicle. An exemplary vehicle includes a processor configured to receive a request signal to replace a first PR bottle. The processor is also configured to transmit an order based on the request signal. The vehicle also includes a plurality of wheels configured to move the vehicle from the first location to a second location, and from the second location to the first location. The vehicle further includes a robotic arm configured to load, at the first location, the first PR bottle into a first container; load a second PR bottle in a second container; remove a cap from the second PR bottle and a socket from the first PR bottle; couple the socket of the first PR bottle to the second PR bottle; and unload the second PR bottle from the second container.

Abstract: Provided herein are methods for making gamma lactones comprising reacting a carboxylic acid substrate with a heterologous cytochrome P450 (CYP450) protein with carboxylic acid 4-hydroxylase activity.

Abstract: Provided herein is a method of producing hydroxytyrosol. The method includes: culturing a recombinant host microorganism in a medium, wherein: the host microorganism has been transformed with: a first polynucleotide sequence encoding a decarboxylase enzyme capable of decarboxylating 4 hydroxyphenylpyruvaic acid (HPP) to produce 4 hydroxyphenylacetaldehyde, and a second polynucleotide sequence encoding a hydroxylase enzyme, wherein the hydroxylase has an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 2, and the medium comprises glucose; and collecting product hydroxytyrosol from one or both of the host microorganism and the culture medium.

Abstract: A request is received from a client device over a Virtual Private Network (VPN) tunnel. The request is received at a first one of a plurality of edge servers of a distributed cloud computing network. A destination of the request is determined and an optimized route for transmitting the request toward an origin server is determined. The optimized route is based at least in part on probe data between edge servers of the distributed cloud computing network. The request is transmitted to a next hop as defined by the optimized route.

Abstract: Provided herein are biosynthetic methods for producing brazzein and related compositions. Uses (e.g., as sweeteners) of the brazzein produced using the methods are also provided.

Abstract: The present disclosure provides esters of a retinoid and bakuchiol, such as esters of Formula (I?). The present disclosure also provides compositions and kits comprising the esters, methods of producing the esters, methods of using the esters (e.g., for treating or preventing a skin disease, slowing the ageing of the skin, improving the appearance of the skin, or modulating a retinoid receptor).

Abstract: The present disclosure relates, at least in part, to the production of delta-lactones and gamma-lactones from fatty acid substrates (e.g., fatty acid substrates of C16-C25) via a batch fermentation method in an engineered microbe using a recombinant cytochrome P450 monooxygenase.

Abstract: The present disclosure relates, at least in part, to the production of delta-lactones from fatty acid substrates via a batch fermentation method in an engineered microbe using a recombinant cytochrome P450 monooxygenase.

Abstract: The present invention relates to novel steviol glycosides rebaudioside R6-5, rebaudioside R6-6, and rebaudioside R7-5 and the production of these novel steviol glycosides, such as through enzymatic bioconversion.

Abstract: Provided herein are biosynthetic methods for producing lactones, such as macrocyclic lactones, from fatty acids. The lactones produced using and/or during the biosynthetic methods are also provided.

Abstract: A request is received from a client device over a Virtual Private Network (VPN) tunnel. The request is received at a first one of a plurality of edge servers of a distributed cloud computing network. A destination of the request is determined and an optimized route for transmitting the request toward an origin server is determined. The optimized route is based at least in part on probe data between edge servers of the distributed cloud computing network. The request is transmitted to a next hop as defined by the optimized route.

Abstract: The present invention provides a novel biosynthetic production process which converts L-galactose into 2?-fucosyllactose via four enzymatically catalyzed reaction steps. The present process is designed such that co-factors required by the process are regenerated within the four reaction steps, hence making the process cost-effective and efficient. The process can be performed in vitro in a cell-free system. The present invention also provides mutant enzymes that can be used to increase production levels of 2?-fucosyllactose, whether using the novel pathway described herein or the mannose-dependent pathway known in the art.

Abstract: Provided herein are methods for making gamma lactones comprising reacting a carboxylic acid substrate with a heterologous cytochrome P450 (CYP450) protein with carboxylic acid 4-hydroxylase activity.

Abstract: The present disclosure generally relates to the production of natural vanillin by biocouversion using isoeugenol as the substrate. More specifically, the present methods utilize a filitgal isoeugenol monooxygenase to catalyze the bioconversion of isoeugenol to vanillin, which can be carried out in a cellular system (e.g., bacteria or yeasts) or in an enzymatic reaction mixture without a cellular system.

Abstract: The present disclosure generally relates to the production of natural vanillin by bioconversion using isoeugenol as the substrate. More specifically, the present methods utilize a fungal isoeugenol monooxygenase encoded by VpIEM gene from Violaceomyces palustris to catalyze the bioconversion of isoeugenol to vanillin, which can be carried out in a cellular system (e.g., bacteria or yeasts) or in an enzymatic reaction mixture without a cellular system.

Abstract: A request is received from a client device over a Virtual Private Network (VPN) tunnel. The request is received at a first one of a plurality of edge servers of a distributed cloud computing network. A destination of the request is determined and an optimized route for transmitting the request toward an origin server is determined. The optimized route is based at least in part on probe data between edge servers of the distributed cloud computing network. The request is transmitted to a next hop as defined by the optimized route.

Abstract: Provided herein are methods for identifying and isolating polynucleotides coding for polypeptides having D-allulose 3-epimerase activity from a wide variety of microorganisms. Also provided are nucleic acid constructs, vectors and recombinant host cells comprising the polynucleotides coding for D-allulose 3-epimerase activity as well as methods for producing allulose from fructose using said recombinant host cells having D-allulose 3-epimerase activity or the D-allulose 3-epimerase enzyme of said recombinant host cells having D-allulose 3-epimerase activity.