Abstract: Provided herein are compositions and methods for detecting exhaled microRNAs for identifying lung diseases such as cancer, asthma, and chronic obstructive pulmonary disease.

Abstract: The present disclosure provides a single mouse exhaled breath collection device and proof-of-concept analyses utilizing an ultra-sensitive and customizable EV purification assay, EV-CATCHER® (Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release) assay. Preliminary studies show that this assay work well for the isolation of exhaled extracellular vesicles (exh-EVs) from exhaled breath condensates (EBC) to capture and characterize miRNAs contained in exh-EVs. Because assembling a large enough cohort of asymptomatic subjects requires time, a complementary approach of using humanized animal models has been chosen to comprehensively assess the robustness and sensitivity of transcriptomic (miRNA and mRNA) and proteomic biomarkers contained in tumor exh-EVs and to enable sensitive detection of human primary and secondary lung cancers during their early development.

Abstract: The present disclosure provides a non-invasive method for early detection of deep lung pathology in a subject at risk of the deep lung pathology. The method comprises (a) sampling deep lung tissue of the subject at risk of the deep lung pathology by isolating micro RNA (riRNA) cargo of lung tissue origin in exhaled extracellular vesicles (EVs) purified from exhaled breath condensates (EBCs) of the subject; (b) purifying lung-specific exhaled extracellular vesicles in exhaled breath condensate by antibody capture of the lung-specific exhaled EVs; and (c) detecting the pathology by comparing an miRNA profile of the exhaled extracellular vesicles of lung tissue origin purified from the subject to the profile of exhaled extracellular vesicles purified from exhaled breath condensates of a healthy subject. The early detection can lead to early treatment of the pathology and an improved health outcome for the subject.

Abstract: A method of purifying Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV-CATCHER), designed for high-throughput analysis of low-abundance small-RNA cargos by next-generation sequencing, and use of this method for preparing purified populations of biological particles or cells from a biological sample for in vitro evaluation of a patient's risk of developing and for treating a severe viral infection; for enhancing therapeutic effectiveness of convalescent plasma therapy in a patient at risk for a severe coronavirus infection, and for treating a patient with a severe coronavirus infection, are described.

Abstract: The present provides methods and kits for extracting DNA and RNA from FFPE tissues and samples.

Abstract: The present provides methods and kits for extracting DNA and RNA from FFPE tissues and samples.

Abstract: This invention provides methods, primers and kits for restoration of nucleic acid from tissue, in particular degraded tissue and formalin-fixed and paraffin-embedded (FFPE) tissue, where the methods involve complementary-template reverse-transcription (CT-RT) where short single-stranded DNA sequences reverse-transcribed from mRNA are used for reverse-transcription of complementary sense-RNA templates. The methods can be used to determine patterns of gene expression and chromosomal alterations in archived tissue samples, and may be used to identify expression of disease-related genes.

Abstract: This invention provides methods, primers and kits for restoration of nucleic acid from tissue, in particular degraded tissue and formalin-fixed and paraffin-embedded (FFPE) tissue, where the methods involve complementary-template reverse-transcription (CT-RT) where short single-stranded DNA sequences reverse-transcribed from mRNA are used for reverse-transcription of complementary sense-RNA templates. The methods can be used to determine patterns of gene expression and chromosomal alterations in archived tissue samples, and may be used to identify expression of disease-related genes.