Patents by Inventor Omid R. Faridani

Omid R. Faridani has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190264284
    Abstract: This application discloses methods for cDNA synthesis with improved reverse transcription, template switching and preamplification to increase both yield and average length of cDNA libraries generated from individual cells. The new methods include exchanging a single nucleoside residue for a locked nucleic acid (LNA) at the TSO 3? end, using a methyl group donor, and/or a MgCl2 concentration higher than conventionally used. Single-cell transcriptome analyses incorporating these differences have full-length coverage, improved sensitivity and accuracy, have less bias and are more amendable to cost-effective automation. The invention also provides cDNA molecules comprising a locked nucleic acid at the 3?-end, compositions and cDNA libraries comprising these cDNA molecules, and methods for single-cell transcriptome profiling.
    Type: Application
    Filed: February 19, 2019
    Publication date: August 29, 2019
    Inventors: Rickard Sandberg, Simone Picelli, Omid R. Faridani
  • Patent number: 10266894
    Abstract: This application discloses methods for cDN?A synthesis with improved reverse transcription, template switching and preamplification to increase both yield and average length of cDNA libraries generated from individual cells. The new methods include exchanging a single nucleoside residue for a locked nucleic acid (INA) at the TSO 3? end, using a methyl group donor, and/or a MgCb concentration higher than conventionally used. Single-cell transcriptome analyses incorporating these differences have full-length coverage, improved sensitivity and accuracy, have less bias and are more amendable to cost-effective automation. The invention also provides cDNA molecules comprising a locked nucleic acid at the 3?-end, compositions and cDNA libraries comprising these cDNA molecules, and methods for single-cell transcriptome profiling.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: April 23, 2019
    Assignee: LUDWIG INSTITUTE FOR CANCER RESEARCH LTD
    Inventors: Rickard Sandberg, Simone Picelli, Omid R. Faridani
  • Publication number: 20160258016
    Abstract: This application discloses methods for cDN?A synthesis with improved reverse transcription, template switching and preamplification to increase both yield and average length of cDNA libraries generated from individual cells. The new methods include exchanging a single nucleoside residue for a locked nucleic acid (INA) at the TSO 3? end, using a methyl group donor, and/or a MgCb concentration higher than conventionally used. Single-cell transcriptome analyses incorporating these differences have full-length coverage, improved sensitivity and accuracy, have less bias and are more amendable to cost-effective automation. The invention also provides cDNA molecules comprising a locked nucleic acid at the 3?-end, compositions and cDNA libraries comprising these cDNA molecules, and methods for single-cell transcriptome profiling.
    Type: Application
    Filed: August 22, 2014
    Publication date: September 8, 2016
    Inventors: Rickard Sandberg, Simone Picelli, Omid R. Faridani