Patents by Inventor Osami Habuchi
Osami Habuchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8288139Abstract: A galactosamine derivative represented by the following formula (1): wherein R1, R2 and R5 each independently represents SO3? or H, and at least one of them represents SO3?; R3 represents H, acetyl or SO3?; R4 represents H, a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkenyl group, a substituted or unsubstituted alkynyl group, a substituted or unsubstituted acyl group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted aralkyl group; X represents O, S, NH or CH2; and represents an ? bond or a ? bond, and a sulfotransferase inhibitor comprising the derivative.Type: GrantFiled: August 19, 2008Date of Patent: October 16, 2012Assignee: Seikagaku CorporationInventors: Osami Habuchi, Hirofumi Nakano, Toshihiko Sawada, Sonoko Fujii, Shiori Ohtake
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Publication number: 20100137564Abstract: A polypeptide of N-acetylglucosamine-6-O-sulfotransferase and a DNA encoding the peptide are provided. The polypeptide is (a) or (b) below: (a) a polypeptide having the amino acid sequence represented by SEQ ID NO: 2; or (b) a polypeptide which includes an amino acid sequence including substitution, deletion, insertion or transposition of one or a few amino acids in the amino acid sequence of (a) and which has an enzymatic activity to transfer a sulfate group from a sulfate group donor to a hydroxyl group at 6 position of an N-acetylglucosamine residue located at a non-reducing end of an oligosaccharide represented the formula I: GlcNAc?1-3Gal?1-4GlcNAc??(I) where GlcNAc represents an N-acetyl-glucosamine residue, Gal represents a galactose residue, ? 1-3 represents a ? 1-3 glycosidic linkage, and ? 1-4 represents a ? 1-4 glycosidic linkage.Type: ApplicationFiled: August 19, 2008Publication date: June 3, 2010Applicant: Aichi PrefectureInventors: Kenji Uchimura, Hideki Muramatsu, Kenji Kadomatsu, Reiji Kannagi, Osami Habuchi, Takashi Muramatsu
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Publication number: 20080318293Abstract: A galactosamine derivative represented by the following formula (1): wherein R1, R2 and R5 each independently represents SO3? or H, and at least one of them represents SO3?; R3 represents H, acetyl or SO3?; R4 represents H, a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkenyl group, a substituted or unsubstituted alkynyl group, a substituted or unsubstituted acyl group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted aralkyl group; X represents O, S, NH or CH2; and represents an ? bond or a ? bond, and a sulfotransferase inhibitor comprising the derivative.Type: ApplicationFiled: August 19, 2008Publication date: December 25, 2008Applicant: SEIKAGAKU KOGYO KABUSHIKI KAISHAInventors: Osami HABUCHI, Hirofumi Nakano, Toshihiko Sawada, Sonoko Fujii, Shiori Ohtake
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Publication number: 20060128659Abstract: A galactosamine derivative represented by the following formula (1): wherein R1, R2 and R5 each independently represents SO3? or H, and at least one of them represents SO3?; R3 represents H, acetyl or SO3?; R4 represents H, a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkenyl group, a substituted or unsubstituted alkynyl group, a substituted or unsubstituted acyl group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted aralkyl group; X represents O, S, NH or CH2; and represents an ? bond or a ? bond, and a sulfotransferase inhibitor comprising the derivative.Type: ApplicationFiled: July 10, 2003Publication date: June 15, 2006Inventors: Osami Habuchi, Hirofumi Nakano, Toshihiko Sawada, Sonoko Fujii, Shiori Ohtake
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Publication number: 20050208631Abstract: A polypeptide of N-acetylglucosamine-6-O-sulfotransferase and a DNA encoding the peptide are provided. The polypeptide is (a) or (b) below: (a) a polypeptide consisting of an amino acid sequence represented by SEQ ID NO: 2; or (b) a polypeptide which comprises an amino acid sequence including substitution, deletion, insertion or transposition of one or few amino acids in the amino acid sequence of (a) and which has an enzymatic activity to transfer a sulfate group from a sulfate group donor to a hydroxyl group at 6 position of an N-acetylglucosamine residue located at a non-reducing end of an oligosaccharide represented by the formula I: GlcNAc?1-3Gal ?1-4GlcNAc??(I) wherein GlcNAc represents an N-acetylglucosamine residue, Gal represents a galactose residue, ?1-3 represents a ?1-3 glycosidic linkage, and ?1-4 represents a ?1-4 glycosidic linkage.Type: ApplicationFiled: October 15, 2004Publication date: September 22, 2005Inventors: Kenji Uchimura, Hideki Muramatsu, Kenji Kadomatsu, Reiji Kannagi, Osami Habuchi, Takashi Muramatsu
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Publication number: 20050142644Abstract: Apolypeptide of N-acetylglucosamine-6-O-sulfotransferase and a DNA encoding the peptide are provided. The polypeptide is (a) or (b) below: (a) a polypeptide consisting of an amino acid sequence represented by SEQ ID NO: 2; or (b) a polypeptide which comprises an amino acid sequence including substitution, deletion, insertion or transposition of one or few amino acids in the amino acid sequence of (a) and which has an enzymatic activity to transfer a sulfate group from a sulfate group donor to a hydroxyl group at 6 position of an N-acetylglucosamine residue located at a non-reducing end of an oligosaccharide represented by the formula I: GlcNAc?1-3Gal?1-4GlcNAc ??(I) wherein GlcNAc represents an N-acetylglucosamine residue, Gal represents a galactose residue, ?1-3 represents a ?1-3 glycosidic linkage, and ?1-4 represents a ?1-4 glycosidic linkage.Type: ApplicationFiled: October 15, 2004Publication date: June 30, 2005Inventors: Kenji Uchimura, Hideki Muramatsu, Kenji Kadomatsu, Reiji Kannagi, Osami Habuchi, Takashi Muramatsu
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Publication number: 20030008366Abstract: Apolypeptide of N-acetylglucosamine-6-O-sulfotransferase and a DNA encoding the peptide are provided.Type: ApplicationFiled: August 5, 2002Publication date: January 9, 2003Inventors: Kenji Uchimura, Hideki Muramatsu, Kenji Kadomatsu, Reiji Kannagi, Osami Habuchi, Takashi Muramatsu
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Patent number: 6455289Abstract: Apolypeptide of N-acetylglucosamine-6-O-sulfotransferase and a DNA encoding the peptide are provided. The polypeptide is (a) or (b) below: (a) a polypeptide consisting of an amino acid sequence represented by SEQ ID NO: 2; or (b) a polypeptide which comprises an amino acid sequence including substitution, deletion, insertion or transposition of one or few amino acids in the amino acid sequence of (a) and which has an enzymatic activity to transfer a sulfate group from a sulfate group donor to a hydroxyl group at 6 position of an N-acetylglucosamine residue located at a non-reducing end of an oligosaccharide represented by the formula I: GlcNAc&bgr;1-3Gal&bgr;1-4GlcNAc (I) wherein GlcNAc represents an N-acetylglucosamine residue, Gal represents a galactose residue, &bgr;1-3 represents a &bgr;1-3 glycosidic linkage, and &bgr;1-4 represents a &bgr;1-4 glycosidic linkage.Type: GrantFiled: December 23, 1999Date of Patent: September 24, 2002Assignee: Seikagaku CorporationInventors: Kenji Uchimura, Hideki Muramatsu, Kenji Kadomatsu, Reiji Kannagi, Osami Habuchi, Takashi Muramatsu
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Patent number: 6372466Abstract: A galactosaminoglycan 4-sulfotransferase having an activity of transferring a sulfate group from a sulfate group donor to a hydroxyl group at the C-4 position of galactosamine residue of a galactosaminoglycan is presented.Type: GrantFiled: June 23, 1999Date of Patent: April 16, 2002Assignee: Seikagaku CorporationInventors: Osami Habuchi, Shinobu Yamauchi, Yukie Hirahara
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Patent number: 6051406Abstract: A polypeptide of keratan sulfate 6-sulfotransferase having the following physicochemical properties:1 action: a sulfate group is transferred from a sulfate group donor to the hydroxyl group at C-6 position of galactose residue of keratan sulfate;2 substrate specificity: a sulfate group is not substantially transferred to chondroitin, chondroitin sulfate A, chondroitin sulfate C, dermatan sulfate and CDSNS-heparin;3 optimum pH: 6.2 to 6.54 activation: an activity is increased by Mn.sup.2+ or Ca.sup.2+ ;5 Km value for 3'-phosphoadenosine 5'-phosphosulfate: about 2.times.10.sup.-7 M; anda DNA coding for the polypeptide.Type: GrantFiled: December 1, 1997Date of Patent: April 18, 2000Assignee: Seikagaku Kogyo Kabushiki KaishInventors: Masakazu Fukuta, Osami Habuchi
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Patent number: 6037159Abstract: Apolypeptide of N-acetylglucosamine-6-O-sulfotransferase and a DNA encoding the peptide are provided. The polypeptide is (a) or (b) below:(a) a polypeptide consisting of an amino acid sequence represented by SEQ ID NO: 2; or(b) a polypeptide which comprises an amino acid sequence including substitution, deletion, insertion or transposition of one or few amino acids in the amino acid sequence of (a) and which has an enzymatic activity to transfer a sulfate group from a sulfate group donor to a hydroxyl group at 6 position of an N-acetylglucosamine residue located at a non-reducing end of an oligosaccharide represented by the formula I:GlcNAc.beta.1-3Gal.beta.1-4GlcNAc (I)wherein GlcNAc represents an N-acetylglucosamine residue, Gal represents a galactose residue, .beta.1-3 represents a .beta.1-3 glycosidic linkage, and .beta.1-4 represents a .beta.1-4 glycosidic linkage.Type: GrantFiled: March 5, 1999Date of Patent: March 14, 2000Assignee: Seikagaku CorporationInventors: Kenji Uchimura, Hideki Muramatsu, Kenji Kadomatsu, Reiji Kannagi, Osami Habuchi, Takashi Muramatsu
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Patent number: 5955325Abstract: Disclosed is a method for producing a sulfated lactosamine oligosaccharide, comprising the step of allowing a sulfotransferase to act on a lactosamine oligosaccharide, the sulfotransferase transferring sulfate group to hydroxyl group at C-6 position of galactose residue in the lactosamine oligosaccharide.Type: GrantFiled: March 31, 1997Date of Patent: September 21, 1999Assignee: Seikagaku Kogyo Kabushiki KaishaInventor: Osami Habuchi
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Patent number: 5910581Abstract: A polypeptide of glycosaminoglycan sulfotransferase originating from human and having the following physical and chemical properties:(i) action: sulfate group is transferred from a sulfate group donor to N-acetylgalactosamine residue or galactose residue of glycosaminoglycan;(ii) substrate specificity: sulfate group is transferred to the hydroxyl group position at C-6 of N-acetylgalactosamine residue of chondroitin; and sulfate group is transferred to the hydroxyl group position at C-6 of galactose residue of keratan sulfate; and(iii) molecular weight: about from 50,000 to 55,000 Da.Type: GrantFiled: July 24, 1997Date of Patent: June 8, 1999Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Osami Habuchi, Masakazu Fukuta
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Patent number: 5834282Abstract: The present invention provides a heparan sulfate 6-O-sulfotransferase for selectively introducing sulfate group into hydroxyl group at C-6 of glucosamine of heparin and heparan sulfate, having the following properties: (i) sulfate group is selectively transferred from a sulfate group donor to hydroxyl group at C-6 of N-sulfoglucosamine residue; (ii) sulfate group is transferred to CDSNS-heparan, but sulfate group is not transferred to chondroitin and chondroitin-4-sulfate; (iii) optimum reaction pH is in a range of pH 6-7; (iv) optimum ionic strength is in a range of 0.1-0.3 M (in the case of sodium chloride); and (v) the enzyme activity is inhibited by dithiothreitol and adenosine-3',5'-diphosphate, and activated by protamine.Type: GrantFiled: July 21, 1995Date of Patent: November 10, 1998Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Hiroko Habuchi, Osami Habuchi, Koji Kimata
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Patent number: 5827713Abstract: Chondrotin 6-sulfotransferase (C6ST), for transferring sulfate group to the hydroxyl group at C-6 position of N-acetylgalactosamine residue or galactose residue of glycosaminoglycan, was purified from a culture liquid of chick embryo chondrocytes to determine its partial amino acid sequences. Partial cDNA of C6ST was amplified from poly(A).sub.+ prepared from the chondrocytes, by means of PCR by using oligonucleotide primers prepared on the basis of the determined sequences. Entire length cDNA of C6ST was obtained from a cDNA library by means of hybridization by using an obtained cDNA fragment as a probe.Type: GrantFiled: May 31, 1996Date of Patent: October 27, 1998Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Osami Habuchi, Masakazu Fukuta
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Patent number: 5817487Abstract: Heparan sulfate 2-O-sulfotransferase having the following properties is obtained from, for example, CHO cells:(i) action: sulfate group is transferred from a sulfate group donor selectively to the hydroxyl group at C-2 position of iduronic acid residue of a sulfate group acceptor;(ii) substrate specificity: sulfate group is transferred to heparan sulfate or CDSNS-heparin, but sulfate group is not transferred to chondroitin, chondroitin sulfate, dermatan sulfate, and keratan sulfate;(iii) optimum reaction pH: about pH 5 to 6.5;(iv) optimum ionic strength: about 50 to 200 mM when sodium chloride is used; and(v) inhibition and activation: the activity of the enzyme is increased by protamine, the activity of the enzyme is inhibited by adenosine-3',5'-diphosphate, and the activity of the enzyme is scarcely affected by dithiothreitol at 10 mM or less.Type: GrantFiled: July 24, 1996Date of Patent: October 6, 1998Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Masashi Kobayashi, Hiroko Habuchi, Osami Habuchi, Koji Kimata