Abstract: This invention is used for the multiplex cells/tissues analysis using 3D cells/tissue culturing functional particles. Functional particle has been used for the variety of biological assays including immunology, biochemistry and molecular biology as well as cell biology. We combined different particles in same reaction vessel for realizing multiple assays for cells or tissues analysis. These particles are identified by their mark as different color or fluorescence. Then differently identified cells/tissues are analyzed according to their response to the outside signals evenly. We can apply any signals as chemical trigger, light activation, electric triggers, mechanical trigger or temperature shift. In this patent application, we extend to use these functional particles with permeable parts or permeable reaction vessel for the high trough put cells and tissues analysis. If the diffusion speed of these are calibrated correctly, we can measure complex kinetic of cells or tissues response.