Abstract: A culture vessel includes at least a first port used for medium supply and a second port used for medium discharge, and A medium supplying vessel serves as a mixing vessel for adjusting a concentration of A new medium to be supplied to the culture vessel. A cell culture method includes: transferring a portion of a used medium in the culture vessel from the culture vessel to the mixing vessel; creating a concentration-adjusted medium by mixing the new medium and the portion of the used medium in the mixing vessel; and filling the culture vessel with the concentration-adjusted medium by discharging a remaining used medium from the culture vessel while the concentration-adjusted medium is transferred from the mixing vessel to the culture vessel.

Abstract: Provided is a cell culture system capable of detecting a proliferative property of cells in a culture vessel at a desired timing during a culture when the cells are statically cultured in the culture vessel formed of a gas permeable member. A cell culture system is a system for statically culturing cells with a culture vessel at least partially formed of a gas permeable member. The cell culture system includes an oxygen concentration sensor that measures an oxygen concentration in a vicinity of a culture surface inside the culture vessel, and a proliferation detection unit that detects a proliferative property of the cells based on an oxygen permeability of the gas permeable member, a measurement value of the oxygen concentration sensor, an oxygen concentration around the culture vessel, and an oxygen consumption per cell of the cells.

Abstract: Provided is a cell culture vessel for culturing cells with a high density having excellent gas permeability and strength. A bag-shaped cell culture vessel of a closed system includes at least one port and mutually opposed planar substrates. At least one of the planar substrates is formed of a gas permeable film, the at least one gas permeable film has an outer surface provided with a protruding portion having a shape different from an inner surface shape of the gas permeable film, a culture space for culturing cells is provided in the inner surface side of the gas permeable film, and when the gas permeable film is brought in contact with a planar surface, a space enabling ventilation is formed between the gas permeable film and the planar surface by the protruding portion.

Abstract: Provided is a culture vessel for adherent cells that has a large culture portion surface area and ensures easily dissociating and recovering cells without consuming a medium or a dissociation solution more than necessary. The bag-shaped adherent cell culture vessel made of flexible packaging material includes a vessel body portion having a first vessel wall and a second vessel wall and one or more injecting/ejecting ports. The vessel body portion has an intermediate culture wall between the first vessel wall and the second vessel wall. A culture chamber is disposed in each of between the first vessel wall and the intermediate culture wall and between the second vessel wall and the intermediate culture wall, and a flow passage communicating between the respective culture chambers is disposed.

Abstract: An adherent cell culture substrate, which is a sheet-shaped substrate used for producing a culture vessel for adherent cells, includes a groove including a crest-shaped portion and a valley-shaped portion on one surface side of the substrate, in which a distance from a top end portion of the crest-shaped portion to the other surface of the substrate is 1 mm or less. Further, it is preferable that a cross section of the groove perpendicular to a direction in which the groove extends is formed in a substantially V-shape and an inclination angle of a side surface of the substantially V-shape of the groove is 80 degrees or less. Further, it is preferable that a plurality of the grooves are linearly arranged in parallel and the top end portions of the crest-shaped portions are formed in a linear shape.

Abstract: A cell culture system includes a culture bag made of a flexible packaging material and having a plurality of ports, a medium container that stores a medium to be transferred to the culture bag, and a control part that controls the supply of the medium, tubular members are connected to the respective ports to circularly connect the culture bag and the medium container through the tubular members, the tubular member connected to a first port of the plurality of ports is provided with first supply unit (e.g., pump), and the tubular member connected to a second port of the plurality of ports is provided with second supply unit (e.g., pump), and the control part controls the operation of at least the first supply unit or the second supply unit to circulate the medium in the culture bag and the medium container.

Abstract: In the production of a culture vessel in which a low cell-adhesive polymer as a coating agent is applied to a culture surface having a plurality of concave parts formed thereon, a use amount of the coating agent can be reduced. The low cell-adhesive polymer as the coating agent is applied to a surface of a culture base material in advance, and subsequently the culture base material is subjected to processing to form the culture vessel having the surface of the culture base material coated with the coating agent as the culture surface.

Abstract: A liquid thickness of a medium in a culture bag used in cell culture can be controlled. A liquid thickness controlling mechanism that controls the liquid thickness of the medium filled in the culture bag provided with at least one port and formed of a soft packing material includes a detection portion that measures a change caused by a change in the liquid thickness of the medium and a control portion that controls operation of liquid delivery means disposed in a tubular member connecting the culture bag and a medium bag based on detection information inputted from the detection portion so as to control delivery of the medium between the culture bag and the medium bag. Using the liquid thickness controlling mechanism makes it possible to control the liquid thickness of the medium in the culture bag being used.

Abstract: In a culture container including a plurality of recesses for culturing a culture target, such as spheres, a liquid substance can flow between the recesses and the movement of the culture target between the recesses can be prevented. A culture container includes a plurality of recesses for accommodating a culture target, the culture container including a container body having a first surface in which the recesses are formed, and a top plate having a second surface facing the first surface, protrusion portions and/or channel portions being provided on a side of the first surface and/or a side of the second surface, the container body being in contact with a part of the top plate to form a channel between the container body and the top plate, and a width of the channel being smaller than a minimum diameter of the culture target.

Abstract: It is possible to efficiently replace a medium, clean spheres, and arrange spheres at regular intervals in the formation of spheres. A member is capable of capturing spheres, the member having a first surface and a second surface on an opposite side of the first surface, and having a plurality of recesses; the recess having an opening formed in the first surface, and one microhole being formed for each opening in the second surface; a circle or inscribed circle of the opening having a diameter a of 50 ?m or more and 1 mm or less, a circle or inscribed circle of the microhole having a diameter b of 1 ?m or more and less than 200 ?m, and a and b satisfying a>b.

Abstract: A cell culture apparatus including cell culture units U and a measurement part (5). The measurement part (5) is provided with an imaging part (51) and is disposed so as to be capable of moving up and down between a back panel part (21) of a casing (2) and a culture container accommodating shelf (32). When the measurement unit (5) reaches a measurement position of each cell culture unit U which is a measurement subject, the culture container accommodating shelf (32) slides toward the measurement unit (5) so that portions to be measured of culture containers U20, U30 in each cell culture unit U come in an imaging range of the imaging part (51).

Abstract: A counting device counts the number of counting objects in liquid in a flexible container. The counting device includes a mounting table on which the container is mounted and a pressing table arranged opposite to a container-arranged side of the mounting table. The mounting table and the pressing table move relatively in a direction of sandwiching the container, and a light-passing hole facing a surface of the container is provided in at least one of the mounting table and the pressing table. The pressing table includes one or more thickness-setting members that sandwich the container between the pressing table and the mounting table in a periphery of the light-passing hole and on the side opposite to the container in a protruding manner.

Abstract: A cell culture apparatus in which on an abutting surface (2a) around an opening part of a thermostatic chamber (3) and a cold storage chamber (4) that abuts against packing elements (3b, 4b) when front doors (3a, 4a) are closed, a communication groove (5) is provided that intercommunicates the inside and outside of the thermostatic chamber (3) by a communication groove (51) when a transfer tube (U40) is removed. The shut-off mechanism (5) is formed of: a guide hole (52) that opens on the abutting surface (2a) across the communication groove (51) and is deeper than the communication groove (51); a shutter member (53) which is inserted into the guide hole (52) and moves between the blocking position blocking the communication groove (51) and the opening position opening the communication groove (51); and a spring member (54) energizing the shutter member (53) to the blocking position.

Abstract: A cell culture apparatus including cell culture units U and a measurement part (5). The measurement part (5) is provided with an imaging part (51) and is disposed so as to be capable of moving up and down between a back panel part (21) of a casing (2) and a culture container accommodating shelf (32). When the measurement unit (5) reaches a measurement position of each cell culture unit U which is a measurement subject, the culture container accommodating shelf (32) slides toward the measurement unit (5) so that portions to be measured of culture containers U20, U30 in each cell culture unit U come in an imaging range of the imaging part (51).

Abstract: A cell culture system includes a culture medium container to supply a culture medium to a culture container, two culture containers for cell culture, and a transfer tube to connect these containers, whereby liquid delivery is performed by one pump. The containers are connected to the transfer tube; the one pump is attached to the transfer tube; the second culture container is connected to the transfer tube on both sides of the pump to connect the second culture container and the transfer tube, to inject cells into the first culture container; the culture medium is transferred from the culture medium container to the first culture container by the pump; after the cell culture in the first culture container, a cell suspension is transferred from the first culture container to the second culture container; and the culture medium is transferred from the culture medium container to the second culture container.

Abstract: An induction heating container (1) in which an induction heat generator (3) is attached to the inside of a container main body (2) made of a non-conductive material so as to enable cooking by heating by an electromagnetic cooker. The induction heat generator (3) includes: a laminated body involving a conductor layer (4) that generates heat by induction of eddy current by high-frequency magnetic field and a heat-seal layer (5) that imparts heat-sealing properties to the container main body, and an eddy current control part (6) obtained by cutting the conductor layer (4) along the circumferential direction.

Abstract: In a method of manufacturing a liquid jet head, a laminated substrate is formed by bonding onto a base substrate piezoelectric substrates having side surfaces joined to each other. Discharge grooves serving as channels for discharging liquid and dummy grooves serving as dummy channels that do not discharge the liquid are formed on a surface of the laminated substrate. The discharge grooves and dummy grooves are alternately arranged relative one another and are parallel to a longitudinal direction of bonding surfaces formed by the joined side surfaces of the piezoelectric substrates. The dummy grooves are formed at the respective bonding surfaces. Drive electrodes are formed on side surfaces of the grooves.

Abstract: A counting device counts the number of counting objects in liquid in a flexible container. The counting device includes a mounting table on which the container is mounted and a pressing table arranged opposite to a container-arranged side of the mounting table. The mounting table and the pressing table move relatively in a direction of sandwiching the container, and a light-passing hole facing a surface of the container is provided in at least one of the mounting table and the pressing table. The pressing table includes one or more thickness-setting members that sandwich the container between the pressing table and the mounting table in a periphery of the light-passing hole and on the side opposite to the container in a protruding manner.

Abstract: To provide an induction heating container that can be used safely in which bumping that occurs when a liquid object to be heated such as water or soup accommodated therein is heated by an electromagnetic cooker is effectively suppressed, thereby preventing a user from getting burned or the surrounding area of the electromagnetic cooker from being contaminated. This induction heating container 1 is an induction heating container in which an induction heat generator 3 is attached to the inside of a container main body 2 that is made of a non-conductive material so as to enable cooking by heating by an electromagnetic cooker, wherein the induction heat generator 3 comprises: a laminated body involving a conductor layer 4 that generates heat by induction of eddy current by high-frequency magnetic field and a heat-seal layer 5 that exhibits heat-sealing properties to the container main body, and an eddy current control part 6 that is obtained by cutting the conductor layer 4 along the circumferential direction.

Abstract: A liquid jet head includes a flow path member having a supply port through which liquid is supplied and a discharge port through which the liquid is discharged, and a cover plate having a liquid supply chamber that communicates with the supply port and a liquid discharge chamber that communicates with the discharge port. An actuator substrate has a plurality of parallel channels that extend between the liquid supply chamber and the liquid discharge chamber and the channels communicate with respective nozzles formed in a nozzle plate. The flow path member, cover plate, actuator substrate and nozzle plate constitute a laminated structure. A communication path is provided in one or both of the cover plate and flow path member for bypassing the liquid from the liquid supply chamber to the liquid discharge chamber so that air bubbles trapped in the liquid can be effectively discharged to the outside.