Abstract: An ion selective monoelectrode complex which is favorably employable to manufacture an ion activity measuring apparatus, has on a common non-electroconductive support sheet, plural ion selective monoelectrodes each of which is composed of an electrode composite consisting of, in order, a silver metal layer, a silver halide layer, an electrolytic material layer, and an ion selective membrane, and an electroconductive terminal which is electrically connected to the silver metal layer and which has an exposed surface, under the condition that the ion selective monoelectrodes are aligned, without electric contact with each other, along an imaginary line bridging the electrode composite and the electroconductive terminal.

Abstract: A composite ion selective electrode comprising a calcium ion selective electrode member and a hydrogen ion selective electrode member which is characterized in that a calcium ion selective membrane of the calcium ion selective electrode member has a thickness of 5 to 30 &mgr;m, and the hydrogen ion selective membrane of the hydrogen ion selective electrode member contains tri-n-dodecyl-amine and trisethylhexyl trimellitate. Each of the calcium ion selective electrode member and the hydrogen ion selective electrode member can be employed singly.

Abstract: An agglutination assay method for quantitatively determination of an analyte in an aqueous liquid sample using particles bearing an anti-analyte. The agglutination is conducted in the porous medium layer of the analysis element. A speedy quantitative analysis of the analyte can be conveniently attained with high sensitivity. When the particle-labeled anti-analyte is contained in the porous medium layer, the anti-analyte can be stored with higher stability in the dry state. A dry analysis element for enabling such analysis method is also provided.

Abstract: The present invention provides a structure comprising a vorticosely fixed string-like support, and plural regions on said string-like support to which a biological material is fixed. By using the structure according to the present invention, a target oligonucleotide having complementarity to an oligonucleotide can efficiently be detected.

Abstract: An object of the present invention is to provide a means for simply performing a gene analysis without performing complicated operations. The present invention provides a method for the detection of a nucleic acid, comprising the steps of: performing a PCR reaction or a reverse transcription reaction by adding a template nucleic acid to a solid support on which a nucleic acid is fixed; and hybridizing the nucleic acid fixed on said solid support with the nucleic acid synthesized by the PCR reaction or the reverse transcription reaction.

Abstract: The present invention provides a measurement chip for a surface plasmon resonance biosensor, which comprises a transparent substrate, a metal membrane located on the transparent substrate and an organic silicon membrane immobilized on the metal membrane and in which the organic silicon membrane is immobilized on the metal membrane via a functional group capable of binding with atoms on the surface of a metal. The measurement chip for a biosensor of the present invention can easily be produced. Using the measurement chip of the present invention, a target substance can be measured with good sensitivity, even if only a small amount of physiologically active substance is immobilized.

Abstract: An object of the present invention is to suppress an electric potential drift as described above and improve pH measurement precision in order to obtain practical measurement precision in the dry-type multilayered film-type pH electrode. The present invention provides a complex pH electrode which has at least two ion-selective electrodes comprising a non-conductive support, a pair of electrode layers, an electrolytic layer and an ion-selective membrane where at least one of the ion-selective electrode is a hydrogen ion-selective electrode, wherein the hydrogen ion-selective membrane is saturated with carbon dioxide gas.

Abstract: This invention provides a continuous blood filtration apparatus capable of filtering a plurality of blood samples continuous by using blood filter units to prepare plasma or serum samples in a short period, which comprises, a conveyor conveying a plurality of blood reservoirs each of which contains a blood sample and in which a suction nozzle of a blood filter unit has been put, a manifold connected to a suction line, couples of a valve and a connector for connecting the manifold to the blood filter unit provided on the end of each branch of the manifold, and a mechanism of moving the blood reservoirs in vertical direction.

Abstract: A DNA chip or its analogue composed of nucleotide derivatives attached to a solid carrier is prepared by method of bringing nucleotide derivatives having a reactive group at each one terminal into contact with a solid carrier having thereon reactive groups in an aqueous phase in the presence of a transferase which is capable of producing a covalent bond by rearrangement of the reactive group of the nucleotide derivative and the reactive group of the solid carrier.

Abstract: The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the lebelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase.

Abstract: The present invention provides a fluorescent substance which is represented by a formula: A-B-C wherein A is a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue, or A is a residue of avidin or streptavidin; B is a divalent linking group or a single bond; and C is a monovalent group derived from a general formula (I) and binds to B at a reactive group present in R1 or R2: wherein R1 and R2 each independently represent an alkyl group that may be substituted with a reactive group capable of covalently bonding to A-B-; R3, R4, R5, and R6 each independently represent an alkyl group, and R3 and R4, and/or R5 and R6 may bind to each other to form a saturated carbon-ring together with a carbon atom(s) to which they bind; V1, V2, V3, V4, V5, V6, V7, V8, V9 and V10 each independently represent a hydrogen atom or a monovalent substituent, and two adjacent groups thereof may bind to form a ring; L1, L2,

Abstract: In the manufacture of an ion selective electrode composed of a non-electroconductive support, a pair of electrodes each of which has a silver metal layer and a silver halide layer, an electrolytic material layer, an ion selective membrane, and a non-electroconductive sheet having a pair of openings for receiving a sample solution and a reference solution, respectively, and having thereon a bridge member for electrically connecting the sample solution received in one opening and the reference solution received in another opening, the ion selective membrane is advantageously divided into two separate portions, one of which is placed in a position above one electrode and another of which is placed in a position above another electrode.

Abstract: The present invention provides a dry analytical element comprising, on a support, a layer containing a reagent capable of precipitating globulin. The present invention can provide a dry analytical element which cal overcome the drawback that the viscosity of a sample varies depending on the globulin concentration and the developing area of the sample varies, and can also avoid an error caused by the reaction between the reagent which changes color upon reaction with albumin, and globulin in the sample.

Abstract: A silver/silver halide electrode composed of a non-electroconductive support sheet, a silver metal layer and a silver halide layer in order, is improved in its sensitivity by making the silver halide layer almost free from heavy metal element, for instance, by oxidizing the silver metal layer on its surface side with an oxidizing compound containing no heavy metal element or a chelated ferric oxidizing compound in the presence of a halide containing no heavy metal element.

Abstract: An object of the present invention is to provide a liquid for assaying &agr;-amylase activity that allows for an assay of &agr;-amylase having an acidic optimum pH with high sensitivity. The present invention provides a liquid for assaying &agr;-amylase activity which comprises 5 mM to 50 mM NaCl and 0.09 mM to 0.9 mM CaCl2.

Abstract: The package of a multilayer film electrode web for an electrolyte slide of the invention comprises a packaging film web and the multilayer film electrode web superposed to each other, wherein the multilayer film electrode web is provided with an ion-selective membrane at central portion of the web, wherein the packaging film web is provided with raised portions on both side ends having a height higher than the thickness of the ion-selective membrane, wherein the ion-selective membrane is arranged to exist between the raised portions on both side ends, which protects the ion-selective membrane from being damaged by a foreign body.

Abstract: According to the present invention, there is provided a reactive solid support having a porous substrate wherein the porous region has a fine pore diameter of about 2 nm to about 1000 nm, a porosity of about 10% to about 90% and a thickness of about 0.01 &mgr;m to about 70 &mgr;m, to the surface of which a group of vinyl sulfonyl groups or their reactive precursor groups are fixed by covalent bond via a linking group, respectively. According to the present invention, a probe of a nucleotide derivative or its analog nucleotide such as an oligonucleotide, a polynucleotide, or a peptide nucleic acid can be fixed in high density with high stability on the surface of a solid support having a porosity.

Abstract: A reactive solid carrier favorably employable for manufacturing DNA chip is composed of a solid carrier and a plurality of vinylsulfonyl groups or their reactive precursors each of which is fixed onto a surface of the solid carrier by covalent bonding via a linking group, and a method for producing a DNA chip is performed by bringing the reactive solid carrier into contact with nucleotide derivatives or their analogues having a reactive group which is reactive with the vinylsulfonyl group or reactive precursor fixed to the solid carrier.

Abstract: An agglutination assay method for quantitatively determination of an analyte in an aqueous liquid sample using particles bearing an anti-analyte. The agglutination is conducted in the porous medium layer of the analysis element. A speedy quantitative analysis of the analyte can be conveniently attained with high sensitivity. When the particle-labeled anti-analyte is contained in the porous medium layer, the anti-analyte can be stored with higher stability in the dry state. A dry analysis element for enabling such analysis method is also provided.

Abstract: The present invention provides a measurement chip for a surface plasmon resonance biosensor, which comprises a transparent substrate, a metal membrane located on the transparent substrate and an organic silicon membrane immobilized on the metal membrane and in which the organic silicon membrane is immobilized on the metal membrane via a functional group capable of binding with atoms on the surface of a metal. The measurement chip for a biosensor of the present invention can easily be produced. Using the measurement chip of the present invention, a target substance can be measured with good sensitivity, even if only a small amount of physiologically active substance is immobilized.