Patents by Inventor Otto S. Wolfbeis
Otto S. Wolfbeis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7067275Abstract: A bioanalytical method for determining catalases and peroxidases using europium ions.Type: GrantFiled: November 11, 2002Date of Patent: June 27, 2006Assignee: Active Motif Chromeon GmbHInventors: Otto S. Wolfbeis, Meng Wu, Zhihong Lin
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Patent number: 6979575Abstract: New reactive dyes are described which can be used to fluorescently label bioorganic molecules such as amino acids, proteins, antibodies, nucleotides and also polymer particles. The color of the new dyes and conjugates thereof can be varied over a wide range. In contrast to known symmetric cyanine dyes, the dyes of the invention contain only one reactive group. Hence the labelling takes place without the interfering cross-linking that occurs with bireactive dyes. Their fluorescence quantum yields are very high (especially in the conjugated form). Conjugates thereof with proteins, oligonucleotides or particles can be used in fluorescence-based analytical methods of determination e.g. in immunoassays, in hybridization assays, in cytometry or for pharmaceutical screening.Type: GrantFiled: November 26, 2002Date of Patent: December 27, 2005Assignee: Chromeon GmbHInventors: Nikolai N. Romanov, Otto S. Wolfbeis
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Patent number: 6911179Abstract: The present invention provides an ozone detecting material comprising a polymer prepared by polymerization of at least one monomer selected from the group consisting of 2-, 3-, 4-halogen substituted anilines or aniline substituted with C1-3-alkyl in N-position, and diphenylamine, formed into a thin film coated on a transparent substrate, waveguide, or optical fiber, or additionally comprising a component selected from electro-conductive fine carbon powder, a semiconductive metal oxide, or a semiconductive polymer material, and a method for detecting ozone comprising the steps of contacting this ozone detecting material with an ozone-containing gas and determining the light absorption rate and/or electrical conductivity of the ozone detecting material at that time.Type: GrantFiled: July 5, 2002Date of Patent: June 28, 2005Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Masanori Ando, Otto S. Wolfbeis, Vladimir M. Mirsky
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Patent number: 6835351Abstract: An optical-chemical sensor which is suitable for the continuous and discontinuous determination by luminescence optics of the concentration of chloride in an aqueous sample and which comprises a luminescence indicator (I) and a polymer (H) carrying the luminescence indicator (I) is characterized in that the luminescence indicator (I) is a non-lipophile acridine or bisacridine compound and the polymer (H) is a linear-chain hydrophile polymer soluble in an organic solvent, whereby it is possible to excite the sensor by commercially available LEDs, to manufacture very large numbers thereof at a moderate cost and in a reproducible way and, preferably, to use it for the determination of physiological chloride concentrations and the sensor furthermore has a wide dynamic measuring range for the determination of chloride; a high sensitivity, stability and reproducibility; a high selectivity for chloride; and a low pH cross-sensitivity.Type: GrantFiled: January 2, 2003Date of Patent: December 28, 2004Assignee: F. Hoffmann-La Roche AGInventors: Christian Huber, Tobias Werner, Otto S. Wolfbeis, Douglas E. Bell, Susannah Young
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Publication number: 20030175988Abstract: Hence the invention concerns a method which can be used to provide a fluorescent label on bioorganic molecules carrying amino groups such as amino acids, proteins, pharmaceutical agents, antibodies, amino group-modified nucleotides and also polymers and polymer particles carrying amino groups by means of a chemical (covalent) binding. The method is based on the reaction of a pyrylium salt located on a fluorophore F with the amino group of a biomolecule or particle according to the described reaction equation. The method is selective, simple to carry out and results in high labelling yields. The spectral properties of the conjugates differ considerably from those of the starting compounds and their fluorescence quantum yields are often considerably increased.Type: ApplicationFiled: November 4, 2002Publication date: September 18, 2003Inventors: Sergey M. Yarmoluk, Olexandr M. Kostenko, Oleksiy Iwanowitch Tolmachev, Otto S. Wolfbeis
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Patent number: 6613282Abstract: An optical-chemical sensor which is suitable for the continuous and discontinuous determination by luminescence optics of the concentration of chloride in an aqueous sample and which comprises a luminescence indicator (I) and a polymer (H) carrying the luminescence indicator (I) is characterized in that the luminescence indicator (I) is a non-lipophile acridine or bisacridine compound and the polymer (H) is a linear-chain hydrophile polymer soluble in an organic solvent, whereby it is possible to excite the sensor by commercially available LEDs, to manufacture very large numbers thereof at a moderate cost and in a reproducible way and, preferably, to use it for the determination of physiological chloride concentrations and the sensor furthermore has a wide dynamic measuring range for the determination of chloride; a high sensitivity, stability and reproducibility; a high selectivity for chloride; and a low pH cross-sensitivity.Type: GrantFiled: July 6, 2001Date of Patent: September 2, 2003Assignee: F. Hoffman-La Roche AGInventors: Christian Huber, Tobias Werner, Otto S. Wolfbeis, Douglas E. Bell, Susannah Young
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Publication number: 20030148534Abstract: The present invention provides an ozone detecting material comprising a polymer prepared by polymerization of at least one monomer selected from the group consisting of aniline, aniline having a halogen substituent at the 2-position, aniline having a halogen substituent at the 3-position, aniline having a halogen substituent at the 4-position, aniline having a C1-3 alkyl substituent at the N-position, toluidine and diphenylamine; and a method for detecting ozone comprising the steps of contacting this ozone detecting material with an ozone-containing gas and determining the light absorption rate and/or electrical conductivity of the ozone detecting material at that time.Type: ApplicationFiled: July 5, 2002Publication date: August 7, 2003Inventors: Masanori Ando, Otto S. Wolfbeis, Vladimir M. Mirsky
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Publication number: 20030124639Abstract: The invention concerns methods of determination using enzymes from the group comprising catalases and peroxidases and using optical indicators from the group comprising europium ligand complexes. The new method is based on the surprising finding that hydrogen peroxide (H2O2) bound as a ligand by certain complexes of the europium (III) ion is available for enzymatic reactions. The consumption of hydrogen peroxide by enzymes results in a change in the optical properties and in particular the luminescence-optical properties of the europium complexes. The new reagents allow an improved detection and determination of catalases and peroxidases as well as of substrates, inhibitors and activators thereof. Antigens and nucleic acid oligomers can also be detected or determined by using catalases or peroxidases as markers in immunological or genetic test methods.Type: ApplicationFiled: November 11, 2002Publication date: July 3, 2003Inventors: Otto S. Wolfbeis, Meng Wu, Zhihong Lin
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Publication number: 20030101803Abstract: An optical-chemical sensor which is suitable for the continuous and discontinuous determination by luminescence optics of the concentration of chloride in an aqueous sample and which comprises a luminescence indicator (I) and a polymer (H) carrying the luminescence indicator (I) is characterized in that the luminescence indicator (I) is a non-lipophile acridine or bisacridine compound and the polymer (H) is a linear-chain hydrophile polymer soluble in an organic solvent, whereby it is possible to excite the sensor by commercially available LEDs, to manufacture very large numbers thereof at a moderate cost and in a reproducible way and, preferably, to use it for the determination of physiological chloride concentrations and the sensor furthermore has a wide dynamic measuring range for the determination of chloride; a high sensitivity, stability and reproducibility; a high selectivity for chloride; and a low pH cross-sensitivity.Type: ApplicationFiled: January 2, 2003Publication date: June 5, 2003Applicant: F. Hoffman-La Roche AGInventors: Christian Huber, Tobias Werner, Otto S. Wolfbeis, Douglas E. Bell, Susannah Young
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Publication number: 20020034826Abstract: An optical-chemical sensor which is suitable for the continuous and discontinuous determination by luminescence optics of the concentration of chloride in an aqueous sample and which comprises a luminescence indicator (I) and a polymer (H) carrying the luminescence indicator (I) is characterized in that the luminescence indicator (I) is a non-lipophile acridine or bisacridine compound and the polymer (H) is a linear-chain hydrophile polymer soluble in an organic solvent, whereby it is possible to excite the sensor by commercially available LEDs, to manufacture very large numbers thereof at a moderate cost and in a reproducible way and, preferably, to use it for the determination of physiological chloride concentrations and the sensor furthermore has a wide dynamic measuring range for the determination of chloride; a high sensitivity, stability and reproducibility; a high selectivity for chloride; and a low pH cross-sensitivity.Type: ApplicationFiled: July 6, 2001Publication date: March 21, 2002Applicant: F. Hoffman-La Roche AGInventors: Christian Huber, Tobias Werner, Otto S. Wolfbeis, Douglas E. Bell, Susannah Young
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Patent number: 6139798Abstract: The sensor membrane of an optical sensor for detection of O.sub.2, H.sub.2 O.sub.2, SO.sub.2 or halogenated hydrocarbons in a sample contains an indicator substance that is homogeneously immobilized in the polymer matrix of the sensor membrane and is, at least indirectly, in contact with the sample, changing at least one of its optical properties upon a change of the parameter to be measured. The indicator substance contains an inorganic salt of a transition metal complex with .alpha.-diimine ligands. The indicator substance is homogeneously distributed in the polymer matrix, which essentially consists of at least one substance belonging to the group of cellulose derivatives, polystyrenes, polytetrahydrofuranes, or their respective derivatives.Type: GrantFiled: April 14, 1997Date of Patent: October 31, 2000Assignee: AVL Medical Instruments AGInventors: Ingo Klimant, Hellfried Karpf, Otto S. Wolfbeis
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Patent number: 6046055Abstract: In a sensor layer for quantitative determination of at least one chemical component in a gaseous or liquid sample medium containing a chromophore which is directly or indirectly responsive to the component being determined by changing its absorption spectrum, and a luminophore which is not responsive to the component being determined, where there is an at least partial overlap between the emission spectrum of the luminophore and the absorption spectrum of the chromophore, and where the energy transfer between luminophore and chromophore produces a measurable change in at least one luminescence characteristic of the luminophore, the luminophore L and the chromophore .GAMMA. are ionic substances with differing electrical charges, which are incorporated in a matrix material that is permeable to the chemical component being determined.Type: GrantFiled: March 29, 1999Date of Patent: April 4, 2000Assignee: AVL Medical Instruments AGInventors: Otto S. Wolfbeis, Tobias Werner, Ingo Klimant, Ute Kosch, Marco Jean-Pierre Leiner
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Patent number: 6001999Abstract: The invention relates to a compound having the general Formula I in which one of the groups R.sub.1,R.sub.2,R.sub.3,R.sub.4,R.sub.5,R.sub.6 and R.sub.7 represents an ionophoric moiety and the remaining groups each independently are hydrogen, a lipophilic or hydrophilic group or a reactive group for coupling to a polymer or a biomolecule, or R.sub.2 forms an aromatic ring system together with R.sub.3 and R.sub.5 forms an aromatic ring system together with R.sub.6.the compound of the invention is useful as a luminescence indicator for cations.Type: GrantFiled: May 27, 1998Date of Patent: December 14, 1999Assignee: AVL Medical InstrumentsInventors: Otto S. Wolfbeis, Jorg Daub, Thomas Gareis, Matthias Kollmannsberger, Stefan Heinl, Tobias Werner, Christian Huber, Andrei Boila-Gockel, Marco Jean Pierre Leiner
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Patent number: 5981746Abstract: The invention relates to a compound having the general Formula I ##STR1## in which one of the groups R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6 and R.sub.7 represents an ionophoric moiety and the remaining groups each independently are hydrogen, a lipophilic or hydrophilic group or a reactive group for coupling to a polymer or a biomolecule, or R.sub.2 forms an aromatic ring system together with R.sub.3 and R.sub.5 forms an aromatic ring system together with R.sub.6.The compound of the invention is useful as a luminescence indicator for alkali ions.Type: GrantFiled: May 27, 1998Date of Patent: November 9, 1999Assignee: AVL Medical InstrumentsInventors: Otto S. Wolfbeis, Jorg Daub, Thomas Gareis, Matthias Kollmannsberger, Stefan Heinl, Tobias Werner, Christian Huber, Andrei Boila-Gockel, Marco Jean Pierre Leiner
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Patent number: 5942189Abstract: In a sensor layer for quantitative determination of at least one chemical component in a gaseous or liquid sample medium containing a chromophore which is directly or indirectly responsive to the component being determined by changing its absorption spectrum, and a luminophore which is not responsive to the component being determined, where there is an at least partial overlap between the emission spectrum of the luminophore and the absorption spectrum of the chromophore, and where the energy transfer between luminophore and chromophore produces a measurable change in at least one luminescence characteristic of the luminophore, the luminophore L and the chromophore .GAMMA. are ionic substances with differing electrical charges, which are incorporated in a matrix material that is permeable to the chemical component being determined.Type: GrantFiled: October 14, 1997Date of Patent: August 24, 1999Assignee: AVL Medical Instruments AGInventors: Otto S. Wolfbeis, Tobias Werner, Ingo Klimant, Ute Kosch, Marco Jean-Pierre Leiner
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Patent number: 5853669Abstract: For the purpose of reversible optical indication of the pH of a sample, a hydrophilic accommodating layer is disposed on a hydrophobic mechanically stable support element, which layer contains the indicator dye proper in an immobilized form. This provides a sensor membrane which can be fabricated in a simple manner and is cost-effective, which has a rapid response time and can also be fabricated as a mass product to be used only once.Type: GrantFiled: January 19, 1996Date of Patent: December 29, 1998Assignee: Merck Patent Gesellschaft mit Beschrankter HaftungInventor: Otto S. Wolfbeis
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Patent number: 5407829Abstract: For quality control of packaged organic substances, preferably packaged foods and drugs, the materials to be examined are brought into contact with a planar optical sensor element which is applied on the inside of the wrapping and responds to a change in the gas composition in the gas space above the sample by a change in color or fluorescence. The change of one of the optical properties of the sensor element is detected visually or opto-electronically.Type: GrantFiled: June 29, 1993Date of Patent: April 18, 1995Assignee: AVL Medical Instruments AGInventors: Otto S. Wolfbeis, Helmut List
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Patent number: 5340722Abstract: A method for the continuous and reversible determination of the concentration of an enzyme substrate such as glucose in an specimen, wherein the specimen is brought into contact with a corresponding enzyme selected from oxidases and oxygenases and to which a flavin coenzyme (FMN, FAD) is bonded, the flavin coenzyme changing to a reduced form by the enzyme substrate and to an oxidized form by molecular oxygen dissolved in the specimen, and the change in the fluorescence spectrum, produced by means of the reduction of the flavin coenzyme, is measured.Type: GrantFiled: March 29, 1993Date of Patent: August 23, 1994Assignee: AVL Medical Instruments AGInventors: Otto S. Wolfbeis, Wolfgang Trettnak
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Patent number: 5238809Abstract: For optical determination of the catalytic enzyme activity of a sample by means of enzyme reactants which are split under the influence of the enzyme to be measured, the enzyme reactant is placed at the end of an optical fiber and brought into contact with the sample to be determined. The measurements are carried out by observing the rate of change in spectral characteristics of the enzyme reactant, or its reaction products, resulting from the enzyme reaction. The method permits measurements of undiluted blood and in-vivo determinations of enzyme activities.Type: GrantFiled: June 4, 1991Date of Patent: August 24, 1993Assignee: AVL Medical Instruments AGInventor: Otto S. Wolfbeis
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Patent number: 5232858Abstract: A method for the quantitative determination of one or more chemical parameters of a sample medium employs a fluorescent agent which is not responsive to the parameter to be determined and which has an excitation spectrum and an emission spectrum, and a closely adjacent chromogenic agent which responds to the parameter of the sample medium to be determined by a change in its absorption spectrum the emission spectrum of the fluorescent agent at least partically overlapping the absorption spectrum of the chromogenic agent. The absorption maximum of chromogenic agent is shifted in accordance with the parameter to be determined, causing a reduction of fluorescence decay time t in the fluorescent agent, thereby enabling quantitative determination of the chemical parameter with improved long-term stability and infrequent calibrations.Type: GrantFiled: March 4, 1992Date of Patent: August 3, 1993Assignee: AVL Medical Instruments AGInventors: Otto S. Wolfbeis, Marco J. Leiner