Abstract: Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.

Abstract: Methods and compositions for preparing a nucleic acid sequencing library are described including (a) transposing an insertion sequence into first fragments of the target nucleic acid, wherein the insertion sequence comprises a hybridization sequence, and wherein the transposing produces nicks in the first fragments; (b) combining in a single mixture (i) the first fragments of the target nucleic acid from (a), (ii) a splint oligonucleotide, and (iii) a population of beads, wherein each bead comprises capture oligonucleotides immobilized thereon, and (c) ligating capture oligonucleotides of individual beads to inserted hybridization sequences of individual first fragments.

Abstract: The present invention relates to a nucleic acid molecule capable of blocking motor protein, a library containing the nucleic acid molecule, a method for constructing the nucleic acid molecule or library containing the same, and an application of the nucleic acid molecule or library containing same.

Abstract: Provided is helicase BCH1X, which comprises an amino acid sequence represented by SEQ ID NO: 1 or 2. Further provided are a complex structure comprising helicase BCH1X and a binding moiety used for binding to a polynucleotide, and a use thereof in the control and characterization of a polynucleotide and in single molecule nanopore sequencing.

Abstract: The present invention provides a helicase BCH2X, comprising an amino acid sequence represented by any one of SEQ ID NOs: 1-3. The present invention also provides a complex structure comprising the helicase BCH2X and a binding moiety for binding polynucleotides. The present invention also provides a use of the helicase BCH2X or the complex structure comprising same in the control and characterization of polynucleotides and single-molecule nanopore sequencing.

Abstract: The present invention provides methods, compositions (e.g., eyedrop or spongarion composition), and uses for alleviating and preventing age-associated vitreous degeneration in a mammal, comprising administrating to the mammal in need thereof a therapeutically effective amount of L-ergothioneine, an analog or derivative thereof, or a pharmaceutically acceptable salt, acid, ester, polymer, analog or derivative thereof, as an active ingredient. In particular, L-ergothioneine is capable of scavenging chronic reactive oxygen species directly and radically to mitigate and prevent HA depolymerization and HA decreasing-viscosity in the mammal's vitreous body; and/or improving vitreous liquefaction in the mammal.

Abstract: Described herein are high coverage single tube Long Fragment Read (stLFR) technology which uses performs stLFR on target DNA fragments that have already been amplified before they are co-barcoded, which provides higher amount of DNA for sequencing and increases sequencing coverage. In some embodiments, the high coverage stLFR described in this application uses two rounds of stLFR. In some embodiments, the target DNA fragments are transposed with transposons having particular positional barcodes that can be used to order sequence reads.

Abstract: The present invention provides methods, compositions (e.g., eyedrop or spongarion composition), and uses for alleviating and preventing age-associated vitreous degeneration in a mammal, comprising administrating to the mammal in need thereof a therapeutically effective amount of L-ergothioneine, an analog or derivative thereof, or a pharmaceutically acceptable salt, acid, ester, polymer, analog or derivative thereof, as an active ingredient. In particular, L-ergothioneine is capable of scavenging chronic reactive oxygen species directly and radically to mitigate and prevent HA depolymerization and HA decreasing-viscosity in the mammal's vitreous body; and/or improving vitreous liquefaction in the mammal.

Abstract: Provided is a method for obtaining a double-stranded sequence by single-stranded rolling circle amplification, comprising: 1) performing rolling circle amplification reaction on single-stranded circular DNA by means of a first primer to obtain an amplified sequence, the first primer being complementary to a partial region of the single-stranded circular DNA, and the single-stranded circular DNA having a break mechanism that can cause the single-stranded circular DNA to ring-open; 2) ring-opening the single-stranded circular DNA by means of the break mechanism to obtain single-stranded linear DNA; and 3) using the single-stranded linear DNA as a second primer and using the amplified sequence obtained in step 1) as a template to perform amplification reaction to obtain an amplified double-stranded sequence.

Abstract: The present invention provides novel methods for preparing a moisture-proof amino acid material. The present invention also provides novel compositions or nutritional supplements comprising the moisture-proof amino acid materials prepared by the methods of this invention.

Abstract: The present invention provides methods, compositions (e.g., eyedrop or spongarion composition), and uses for alleviating and preventing age-associated vitreous degeneration in a mammal, comprising administrating to the mammal in need thereof a therapeutically effective amount of L-ergothioneine, an analog or derivative thereof, or a pharmaceutically acceptable salt, acid, ester, polymer, analog or derivative thereof, as an active ingredient. In particular, L-ergothioneine is capable of scavenging chronic reactive oxygen species directly and radically to mitigate and prevent HA depolymerization and HA decreasing-viscosity in the mammal's vitreous body; and/or improving vitreous liquefaction in the mammal.

Abstract: A method for obtaining a single-cell mRNA sequence, including: (1) capturing mRNA of a cell by using a cell tag carrier, and performing reverse transcription to obtain cDNA having a cell tag; (2) obtaining multiple cDNA fragments having molecular tags by using a transposase complex and a molecular tag carrier; (3) performing high-throughput sequencing; (4) performing sequence assembly according to the molecular tags to obtain the sequence of each mRNA; and (5) obtaining the sequence of all mRNAs of each single cell according to the cell tags.

Abstract: Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.

Abstract: Embodiments of the disclosure provide a dissolvable or degradable artificial circulation system for engineering, culturing, and integrating large volume tissues. Also provided are methods of using large engineered tissues prepared using the degradable artificial circulation system for clinical applications and for various applications such as large-scale production of therapeutic or consumable products, drug discovery, and toxicity screening.

Abstract: Described herein are high coverage single tube Long Fragment Read (stLFR) technology which uses performs stLFR on target DNA fragments that have already been amplified before they are co-barcoded, which provides higher amount of DNA for sequencing and increases sequencing coverage. In some embodiments, the high coverage stLFR described in this application uses two rounds of stLFR. In some embodiments, the target DNA fragments are transposed with transposons having particular positional barcodes that can be used to order sequence reads.

Abstract: A method for constructing a sequencing library, a sequencing library and a sequencing method.

Abstract: Methods and compositions for preparing a nucleic acid sequencing library are described including (a)transposing an insertion sequence into first fragments of the target nucleic acid, wherein the insertion sequence comprises a hybridization sequence, and wherein the transposing produces nicks in the first fragments; (b) combining in a single mixture (i) the first fragments of the target nucleic acid from (a), (ii) a splint oligonucleotide, and (iii) a population of beads, wherein each bead comprises capture oligonucleotides immobilized thereon, and (c) ligating capture oligonucleotides of individual beads to inserted hybridization sequences of individual first fragments.

Abstract: Disclosed is a method for obtaining a single-cell mRNA sequence. The method of the present invention comprises: (1) capturing mRNA of a cell by using a cell tag carrier, and performing reverse transcription to obtain cDNA having a cell tag, cDNAs from the same cell having the same cell tag, and cDNAs from different cells having different cell tags; (2) obtaining multiple cDNA fragments having molecular tags by using a transposase complex and a molecular tag carrier, the fragments from the same cDNA having the same molecular tag, and the fragments from different cDNAs having different molecular tags; (3) performing high-throughput sequencing; (4) performing sequence assembly according to the molecular tags to obtain the sequence of each mRNA; and obtaining the sequence of all mRNAs of each single cell according to the cell tags. The method provided by the present invention can be used for obtaining the sequence of all mRNAs of each of a large number of single cells by means of high throughput.

Abstract: Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.

Abstract: Disclosed is a method for constructing a long fragment DNA library, comprising the following steps: 1) breaking a long fragment DNA into target fragments of 3-10 kb by transposase, then amplifying the target fragments, and obtaining target fragment amplification products containing dUTP; 2) amplifying the dUTP in the products by removing the target fragments, fragmenting the target fragments secondarily into DNA short fragments of 300-1200 bp; 3) connecting both ends of the DNA short fragments with sequencing linker single chains A and sequencing linker single chains B respectively; and obtaining connecting sequencing linker products; and 4) PCR amplifying the connecting sequencing linker products, to obtain amplification products.