Abstract: An imaging system uses a multi-view reflector (MVR) to simultaneously measure the three-dimensional (3D) position and orientation of a light emitter. The MVR is positioned at a back focal plane (BFP) of a light collecting optical system, and uses pyramid reflectors and/or conical reflectors to reflect light in at least two regions of the BFP to at least two detection channels of a detector. The at least two detection channels produce images that are used to determine the 3D position and orientation of a light emitter. The system may be used, for example, to image the structure of molecules or organelles in a cell, track the movement of molecules in a cell, or to study the interaction of molecules within a cell.

Abstract: Systems and methods for use in tri-spot point spread function imaging are provided that include a phase mask. The phase mask includes three partitions that each include a subset of the total area that is asymmetrical to other partitions. Each partition includes a phase delay ramp aligned along a phase delay axis, and includes a gradient of phase delays. Each phase delay axis is oriented in a different direction with respect to each other. Included is a source that outputs an excitation beam into a sample containing at least one light emitter that emits a radiation pattern when illuminated. Included is at least one sensor arranged to capture at least one image of the radiation pattern and a phase mask positioned between the at least one emitter and the at least one sensor. The phase mask is configured to produce a tri-spot point spread function.

Abstract: Systems and methods for use in tri-spot point spread function imaging are provided that include a phase mask. The phase mask includes three partitions that each include a subset of the total area that is asymmetrical to other partitions. Each partition includes a phase delay ramp aligned along a phase delay axis, and includes a gradient of phase delays. Each phase delay axis is oriented in a different direction with respect to each other. Included is a source that outputs an excitation beam into a sample containing at least one light emitter that emits a radiation pattern when illuminated. Included is at least one sensor arranged to capture at least one image of the radiation pattern and a phase mask positioned between the at least one emitter and the at least one sensor. The phase mask is configured to produce a tri-spot point spread function.