Abstract: Biomanufacturing hygiene control in batch and fed-batch bioreactors is provided using a bioreactor employing a chlorite/Cld system, wherein the bioreactor comprises an engineered cultured cell expressing a recombinant cytoplasmic chlorite dismutase (cCld) sufficient to increase chlorite resistance of the cell, and chlorite sufficient to substantially inhibit growth of one or more contaminating microorganisms in the bioreactor yet not substantially inhibit growth of the cell.

Abstract: System and methods for continuous culture comprise performing a first continuous culture of cells in a chamber of a first bioreactor under culture conditions that produce cell growth; moving culture fluid comprising cells from the chamber of the first bioreactor into a chamber of a second bioreactor though one or more fluidic conduits; and performing, in the chamber of the second bioreactor, a second continuous culture of cells under culture conditions that produce at least one culture product. The process is performed under conditions wherein the rate of cell growth in the first continuous culture is greater than that of the second continuous culture, and the production of culture product in the first continuous culture is less than that of the second continuous culture.

Abstract: Methods and systems for iteratively improving culture condition for at least one culture output in a culture process. The methods comprise: a) providing a continuous culture of cells in a bioreactor, wherein the cells are cultured under culture conditions that comprise a set of culture parameters. and wherein the culture of cells produces at least one culture output at a first measure: b) while maintaining the cells in continuous culture, iteratively testing different culture conditions by: (i) selecting a new culture condition comprising a new set of culture parameters predicted to improve the measure of the culture output: (ii) perturbing the cells with the new culture conditions: and (iii) determining a new measure of the culture output: wherein testing is iterated to produce a plurality of improvements in the measure of culture output. The iterative process can be performed by an automated artificial intelligence.

Abstract: Biomanufacturing hygiene control in batch and fed-batch bioreactors is provided using a bioreactor employing a chlorite/Cld system, wherein the bioreactor comprises an engineered cultured cell expressing a recombinant cytoplasmic chlorite dismutase (cCld) sufficient to increase chlorite resistance of the cell, and chlorite sufficient to substantially inhibit growth of one or more contaminating microorganisms in the bioreactor yet not substantially inhibit growth of the cell.