Abstract: Provided herein is a method for modifying a polynucleotide or an analogue or derivative thereof, comprising reacting the polynucleotide or analogue or derivative thereof with a compound of formula (I), wherein RH, L1, #, R, n and RP are as defined or derivative thereof, uses of a compound of formula (I), and such compounds per se.

Abstract: Provided herein is a method of producing a polynucleotide or an analogue or derivative thereof, comprising providing a support-attached nucleoside, nucleotide, polynucleotide or derivative or analogue thereof, which is a compound of formula (I) as defined herein and attached to a solid support at (II), (ii) increasing the chain length of the support-attached nucleoside, nucleotide, polynucleotide or derivative or analogue thereof by introducing one or more additional nucleoside units, nucleotide units and/or analogues or derivatives thereof; and (iii) cleaving the resulting polynucleotide from the solid support by photo-illuminating the compound of formula (I). Also provided are associated compounds, uses, and polynucleotides obtainable by such methods.