Abstract: Methods of producing C4 dicarboxylic acids are disclosed. Nucleotide sequences encoding pyruvate carboxylase and uses of such nucleotide sequences in the production of C4 dicarboxylic acids are further disclosed.

Abstract: Methods of producing C4 dicarboxylic acids are disclosed. Nucleotide sequences encoding pyruvate carboxylase and uses of such nucleotide sequences in the production of C4 dicarboxylic acids are further disclosed.

Abstract: The present invention is directed towards the fermentative production of amino acids, providing microorganisms, methods and processes useful therefor. Microorganisms of the invention are capable of converting glucose to amino acids other than L-isoleucine, L-leucine and L-valine with greater efficiency than the parent strain. The efficiency of conversion may be quantified by the formula: [(g amino acid produced/g dextrose consumed)*100]=% Yield and expressed as yield from dextrose. The invention provides microorganisms that are made auxotrophic or bradytrophic for the synthesis of one or more branched chain amino acids by mutagenesis and selected for their ability to produce higher percent yields of the desired amino acid than the parental strain. Preferred microorganisms are Corynebacterium, Brevibacterium or Escherichia coli producing L-lysine. Mutagenesis is performed by classical techniques or through rDNA methodology.

Abstract: Nucleotide sequences and genetic constructs that can be used to regulate genes encoding enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.

Abstract: The present invention is directed towards the fermentative production of amino acids, providing microorganisms, methods and processes useful therefor. Microorganisms of the invention are capable of converting glucose to amino acids other than L-isoleucine, L-leucine and L-valine with greater efficiency than the parent strain. The efficiency of conversion may be quantified by the formula: [(g amino acid produced/g dextrose consumed)*100]=% Yield and expressed as yield from dextrose. The invention provides microorganisms that are made auxotrophic or bradytrophic for the synthesis of one or more branched chain amino acids by mutagenesis and selected for their ability to produce higher percent yields of the desired amino acid than the parental strain. Preferred microorganisms are Corynebacterium, Brevibacterium or Escherichia coli producing L-lysine. Mutagenesis is performed by classical techniques or through rDNA methodology.