Abstract: The invention concerns new compounds for making a hydrophilic surface lipophilic. The compounds have the general formula ##STR1## wherein Z.sup.1 and Z.sup.2 are independently chlorine, fluorine, bromine, alkoxy with not more than 6 carbon atoms, NH, --NH.sub.2, --NH.sub.2 ', wherein R' is alkyl with 1 to 3 carbon atoms, --SH, --CN, --N.sub.3 or hydrogen, and R.sup.1 is ##STR2## wherein each of the S-substituents, S.sub.1, S.sub.2, S.sub.3, S.sub.4 and S.sub.5 are independently selected from the group consisting of hydrogen, alkyl with 1 to 4 carbon atoms, methoxy, ethoxy, and cyano, provided that at least one of the S-substituents is other than hydrogen and when there is a methyl or methoxy S-substituent then (i) at least two of the S-substituents are other than hydrogen, (ii) two adjacent S-substituents form with the phenyl nucleus a naphtalene or anthracene group, or three adjacent S-substituents form together with the phenyl nucleus a pyrene group, and X is the group --(CH.sub.2).sub.

Abstract: The object of the present invention is a new method for fluorometrically assaying the activity of fat-degrading enzymes, such as lipases and phospholipases in samples containing said enzyme, such as in serum. According to the method the enzyme containing sample is reacted with a substrate containing acyl- or alkylglycerols or -phosphoglycerols having at least one fluorescent group, such as a pyrene group. The compounds may in addition contain flurorescence quenching groups. The enzyme hydrolyzes the substrate thus giving rise to changes in the fluorescence intensity during the enzyme reaction and the changes in the intensity are measured at a specific emission wavelength of the fluorescent group employed. The rate of change of the invensity is proportional to the enzyme activity in the sample.The invention concerns also the new compounds containing fluorescent groups to be used in the method.

Abstract: The object of the present invention is a novel and simple process for the production of 1,2-diacyl-sn-glycerols, wherein the acyl groups may be the same or different. According to the invention D-mannitol is reacted with benzene boronic acid or a derivative thereof to form a D-mannitol-monobenzeneboronate which compound is acylated either to form, after removal of the benzeneboronate protective group, a 1,2,5,6-tetraacyl-D-mannitol with four identical acyl groups, or is acylated selectively in two stages, by first acylating the 1,6-hydroxy groups of the boronate protected D-mannitol to form, after removal of the boronate protection, a 1,6-diacyl-D-mannitol, which then is repeatedly reacted with a benzene boronic acid or a derivative thereof and then acylated in the 2- and 5-positions.