Abstract: Recurrent C. difficile infections are the major cause of death due to C. difficile, which is the causative agent of approximately 20% of antibiotic-associated diarrheas. Conventional treatments for C. difficile infections are not capable of eliminating the rates of recurrence, which occurs in 20-30% of the cases and may be repetitive, the probability of death being greater in each cycle. Until now, the persistence mechanisms of C. difficile for producing recurrence conditions were unknown. In the present invention, we describe the mechanism by which C. difficile spores persist, and a method of treatment with a pharmacological composition based on an antibiotic and nystatin for preventing recurrent C. difficile infections. The route of administration of nystatin is also protected (i.e., oral, intraperitoneal).