Abstract: An ultrasonic puncture guidance planning system based on a multi-modal medical image registration, which includes: an MRI sequence image rendering unit configured to receive MRI sequence images, and perform three-dimensional rendering and plot puncture points; an ultrasound sequence image reconstruction unit configured to receive ultrasound sequence images and reconstruct ultrasound volume data; a registration unit configured to perform a three-dimensional registration operation to obtain a registration transformation coefficient; and a puncture planning unit configured to receive ultrasound images of a human body acquired by an ultrasound probe, and generate and display a puncture planning image according to a system puncture point and a current ultrasonic image.