Abstract: Methods and devices for quantifying creatinine in a test sample are provided. The test sample is contacted with a sensing composition to obtain a product comprising a hydantoin-transition metal complex and ammonia. The sensing composition comprises creatinine deaminase and a transition metal salt. The creatinine deaminase enzymatically reacts with creatinine to provide the N-methyl hydantoin and ammonia. The N-methyl hydantoin forms the hydantoin-transition metal complex with the transition salt. A potential difference is applied to the product to measure a current signal provided by the hydantoin-transition metal complex. Concentration of N-methyl hydantoin is obtained based on the measured current signal using a calibration equation. The concentration of N-methyl hydantoin is correlated with concentration of creatinine to quantify the creatinine in the test sample.

Abstract: Methods and devices for quantifying creatinine in a test sample are provided. The test sample is contacted with a sensing composition to obtain a product comprising a hydantoin-transition metal complex and ammonia. The sensing composition comprises creatinine deaminase and a transition metal salt. The creatinine deaminase enzymatically reacts with creatinine to provide the N-methyl hydantoin and ammonia. The N-methyl hydantoin forms the hydantoin-transition metal complex with the transition salt. A potential difference is applied to the product to measure a current signal provided by the hydantoin-transition metal complex. Concentration of N-methyl hydantoin is obtained based on the measured current signal using a calibration equation. The concentration of N-methyl hydantoin is correlated with concentration of creatinine to quantify the creatinine in the test sample.