Abstract: Processes for preparing ion selective membranes are presented which have selectivity for a primary analyte (in particular magnesium) as well as another ion (in particular calcium), wherein said process comprises addition of a) an ionophore (in particular 1,10-phenanthroline), b) a lipophilic salt containing said primary analyte (in particular hemi-magnesium bis[4-octylphenyl]phosphate) and c) a lipophilic salt containing said other ion (in particular hemi-calcium bis[4-octylphenyl]phosphate). Also, ion selective membranes are presented which are prepared using said processes, in particular to membranes selective for magnesium ions. In further aspects, electrodes and potentiometric sensors are presented comprising such membranes and the use thereof for determining ion concentrations in samples.

Abstract: Magnesium ion selective electrode membranes and the preparation thereof. The membranes are rendered highly selective for magnesium ions by the addition of acidic groups to the preferably PVC membrane, either by introducing a lipophilic compound comprising an acidic group covalently linked to a C4-C18 alkyl-substituted phenyl group (e.g. bis-4-octylphenyl phosphoric acid) into the membrane comprising the magnesium selective ionophore (e.g. a neutral ionophore 1,10-phenanthroline derivative) or by covalently linking an acidic (e.g. a carboxylic) group to the ionophore (e.g. a 1,10- phenanthroline derivative).

Abstract: The invention relates to a mutant polypeptide having creatine amidinohydrolase activity, where the polypeptide both retains thermostable activity in the presence of reagents that modify thiol groups. The invention further relates to methods for producing the mutant polypeptide; and to a sensor comprising the mutant polypeptide for use in the measurement of creatinine in samples of physiological fluids. Additionally, the invention teaches how to use the mutant polypeptide to enhance the life-time of a creatinine sensor, and a method for producing the sensor.

Abstract: The invention relates to a mutant polypeptide having creatine amidinohydrolase activity, where the polypeptide both retains thermostable activity in the presence of reagents that modify thiol groups. The invention further relates to methods for producing the mutant polypeptide; and to a sensor comprising the mutant polypeptide for use in the measurement of creatinine in samples of physiological fluids. Additionally, the invention teaches how to use the mutant polypeptide to enhance the life-time of a creatinine sensor, and a method for producing the sensor.

Abstract: The properties of dough or bread can be improved by the addition of a carbohydrate oxidase which can oxidize the reducing end of an oligosaccharide more efficiently than the corresponding monosaccharide, e.g., preferentially oxidizing maltodextrins or cellodextrins over glucose. A novel carbohydrate oxidase having the capability to oxidize maltodextrins and cellodextrins more efficiently than glucose may be obtained from a strain of Microdochium, particularly M. nivale. The amino acid sequence of the novel carbohydrate oxidase has very low homology (<20% identity) with known amino acid sequences.

Abstract: The invention provides a sporocidal composition comprising a laccase or a compound exhibiting laccase activity, a source of oxygen, a source of iodide ions and an enhancing agenL A method of killing or inactivating spores and a method of decontaminating a location, which has been exposed to spores, are also disclosed.

Abstract: The properties of dough or bread can be improved by the addition of a carbohydrate oxidase which can oxidize the reducing end of an oligosaccharide more efficiently than the corresponding monosaccharide, e.g., preferentially oxidizing maltodextrins or cellodextrins over glucose. A novel carbohydrate oxidase having the capability to oxidize maltodextrins and cellodextrins more efficiently than glucose may be obtained from a strain of Microdochium, particularly M. nivale. The amino acid sequence of the novel carbohydrate oxidase has very low homology (<20% identity) with known amino acid sequences.

Abstract: The properties of dough or bread can be improved by the addition of a carbohydrate oxidase which can oxidize the reducing end of an oligosaccharide more efficiently than the corresponding monosaccharide, e.g., preferentially oxidizing maltodextrins or cellodextrins over glucose.

Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having haloperoxidase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences.

Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having haloperoxidase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences.

Abstract: The present invention relates to isolated polypeptides having haloperoxidase activity. The invention also relates to methods for producing and using the polypeptides.

Abstract: 30 The present invention relates to isolated polypeptides having haloperoxidase activity.

Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having haloperoxidase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences.

Abstract: The present invention relates to isolated polypeptides having haloperoxidase activity. The invention also relates to methods for producing and using the polypeptides.

Abstract: By analyzing the three-dimensional structure of the Coprinus laccase structural parts or specific amino acid residues can be identified, which from structural or functional considerations appear to be important for the oxidative stability of a laccase. When comparing the three-dimensional structure of the Coprinus laccase structure with known amino acid sequences of various laccases, it has been found that several similarities exist between the sequences.

Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having haloperoxidase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences.

Abstract: Haloperoxidases are capable of exhibiting excellent oxidizing, bleaching, and antimicrobial activity, when combined with carboxylic acids. Accordingly, the invention provides an enzymatic composition comprising a haloperoxidase, a source of hydrogen peroxide, and a source of a carboxylic acid.

Abstract: The present invention relates to an enzymatic method for killing or inhibiting microbial cells or microorganisms, e.g. in laundry, on hard surfaces, in water systems, on skin, on teeth or on mucous membranes. The present invention also relates to the use of said enzymatic composition for preserving food products, cosmetics, paints, coatings, etc.

Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having haloperoxidase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences.

Abstract: The present invention provides a process for removal of excess dye from newly manufactured printed or dyed fabric or yarn, comprising treatment with a rinse liquor comprising at least one enzyme selected from the group consisting of enzymes exhibiting peroxidase activity or laccase activity, an oxidation agent, and at least one mediator comprising N-hydroxyacetanilide.