Abstract: The present disclosure relates to compounds of formula (I) and pharmaceutical compositions and their use in reducing Widely Interspaced Zinc Finger Motifs (WIZ) expression levels, or inducing fetal hemoglobin (HbF) expression, and in the treatment of inherited blood disorders (e.g., hemoglobinopathies, e.g., beta-hemoglobinopathies), such as sickle cell disease and beta-thalassemia.

Abstract: This disclosure pertains to performance enhancing conditioning and storage solvents containing low levels of buffer and salt for reproducible and improved size exclusion chromatography (SEC). In some embodiments, the present disclosure pertains to storage solvents for protein-based size exclusion chromatography that include water, a water-miscible organic solvent, a phosphate salt, and a neutral salt. In some embodiments, the present disclosure pertains to columns for protein-based size exclusion chromatography that include porous particles and such a storage solvent.

Abstract: A backlight includes a substrate, a plurality of light sources proximate the substrate, a first reflective layer on the substrate, and a plurality of patterned reflectors over the plurality of light sources. Each light source includes a size measured in a plane parallel to the substrate. Each patterned reflector is aligned with a corresponding light source and includes a thickness profile. The thickness profile includes a substantially flat section and a curved section extending from and surrounding the substantially flat section. The substantially flat section varies in thickness by no more than plus or minus 20 percent of an average thickness of the substantially flat section. The substantially flat section includes a size in a plane parallel to the substrate equal to or greater than the size of each light source.

Abstract: Provided herein are immunofluorescence assays that do not use an intensely colored lipophilic dye, and that result in a significant reduction of fluorescence due to aldehyde fixation, collagen, elastin, and/or red blood cells. In certain embodiments of the present invention, a two-step treatment of the tissue is contemplated that leads to the incorporation of reduced heteropoly acids or salts thereof (e.g., phosphomolybdate species) in the tissue. This reagent binds tightly to hydrophilic regions of the tissue and effectively quenches the fluorescence in those regions. In doing so, the overall signal-to-noise ratio for the immunofluorescence assay is increased.

Abstract: Disclosed are compositions, methods, systems, and kits for the detection of markers of interest, which may allow for sequential multiplexing. In some cases, the methods comprise sequential staining of biological samples with detection sets and removal of bound detection agents which may allow for sequential staining with additional detection agents.

Abstract: Provided herein are immunofluorescence assays that do not use an intensely colored lipophilic dye, and that result in a significant reduction of fluorescence due to aldehyde fixation, collagen, elastin, and/or red blood cells. In certain embodiments of the present invention, a two-step treatment of the tissue is contemplated that leads to the incorporation of reduced heteropoly acids or salts thereof (e.g., phosphomolybdate species) in the tissue. This reagent binds tightly to hydrophilic regions of the tissue and effectively quenches the fluorescence in those regions. In doing so, the overall signal-to-noise ratio for the immunofluorescence assay is increased.

Abstract: Provided herein are immunofluorescence assays that do not use an intensely colored lipophilic dye, and that result in a significant reduction of fluorescence due to aldehyde fixation, collagen, elastin, and/or red blood cells. In certain embodiments of the present invention, a two-step treatment of the tissue is contemplated that leads to the incorporation of reduced heteropoly acids or salts thereof (e.g., phosphomolybdate species) in the tissue. This reagent binds tightly to hydrophilic regions of the tissue and effectively quenches the fluorescence in those regions. In doing so, the overall signal-to-noise ratio for the immunofluorescence assay is increased.

Abstract: The present disclosure generally relates to methods for detecting one or more analytes in a sample using immunoassays. In some embodiments, the disclosure provides methods that include quenching the free secondary antibody with a fractionated serum composition. In some other aspects, the disclosure provides systems and kits suitable for carrying out the foregoing methods and any embodiments thereof.