Abstract: A method of generating transcribed nucleic acids, comprising the steps of hybridizing an oligonucleotide probe to a nucleic acid template, wherein said oligonucleotide probe comprises a noncomplementary part in 5? direction of the complementary part that does not hybridize to the nucleic acid template and comprises a sequence of a transcription promoter, hydrolysing a 3? part of the nucleic acid template that is in 3? direction to a part of the nucleic acid template that hybridizes to the oligonucleotide probe and wherein said 3? part is not hybridized to the oligonucleotide probe, extending the nucleic acid template with nucleic acids complementary to the non-complementary part of the oligonucleotide probe, thereby generating a duplex of the sequence of the transcription promoter in sequence with the nucleic acid template, and transcribing the nucleic acid template with a transcriptase that binds the duplex of the sequence of the transcription promoter.

Abstract: A method for generating labelled amplification fragments of a nucleic acid template including the steps of providing a template nucleic acid, annealing at least one oligonucleotide primer to the template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner thereby creating an elongation product, wherein said elongating reaction stops when the elongation product reaches the 5? end of the template nucleic acid or a nucleic acid elongation stopper that is annealed to the template nucleic acid downstream of the elongation product, providing an adaptor nucleic acid that has an identification sequence on its 5? end, wherein the identification sequence does not hybridize to the elongation stopper when in contact thereto, and ligating the adaptor nucleic acid at its 5? end to the 3? end of the elongation product, thereby generating a labelled amplification fragment.

Abstract: A method of ligating DNA molecules, wherein the DNA molecules are in a hybrid with an RNA molecule, including the steps of providing DNA molecules that are in a RNA:DNA hybrid with an RNA molecule, and ligating the DNA molecules to each other with a double strand specific ligase

Abstract: A method of ligating DNA molecules, wherein the DNA molecules are in a hybrid with an RNA molecule, including the steps of providing DNA molecules that are in a RNA:DNA hybrid with an RNA molecule, and ligating the DNA molecules to each other with a double strand specific ligase.

Abstract: The present invention relates to methods for generating an amplified nucleic acid portion of a template nucleic acid, comprising obtaining said template nucleic acid, annealing at least one oligonucleotide primer to said template nucleic acid, annealing at least one oligonucleotide stopper to said template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner until the elongating product nucleic acid reaches the position of an annealed oligonucleotide stopper, whereby the elongation reaction is stopped, wherein in said elongation reaction said oligonucleotide stopper is not elongated, and wherein the elongated product nucleic acid is ligated to the 3? end of said oligonucleotide stopper—said stopper may also be a primer itself and uses and kits for performing said method.

Abstract: The present invention provides a method for generating an amplified nucleic acid portion of a template RNA molecule, comprising after having obtained a template RNA, annealing a first oligonucleotide primer at a preselected 3? terminal nucleic acid region of the template RNA, elongating the first oligonucleotide primer in a template specific manner thereby obtaining a first elongated strand, removing the RNA template, annealing one or more further oligonucleotide primers to the first elongated strand, elongating the one or more further oligonucleotide primers in a template specific manner without strand displacement of polynucleotides annealed to the first elongated strand or using a polymerase that destroys a displaced strand, thereby generating further elongation products, isolating and/or amplifying an elongation product of said further elongation product comprising a nucleic acid portion that is elongated complementary to the first oligonucleotide primer; as well as kits for performing the method.

Abstract: The present invention provides a method for generating an amplified nucleic acid portion of a template RNA molecule, comprising after having obtained a template RNA, annealing a first oligonucleotide primer at a preselected 3? terminal nucleic acid region of the template RNA, elongating the first oligonucleotide primer in a template specific manner thereby obtaining a first elongated strand, removing the RNA template, annealing one or more further oligonucleotide primers to the first elongated strand, elongating the one or more further oligonucleotide primers in a template specific manner without strand displacement of polynucleotides annealed to the first elongated strand or using a polymerase that destroys a displaced strand, thereby generating further elongation products, isolating and/or amplifying an elongation product of said further elongation product comprising a nucleic acid portion that is elongated complementary to the first oligonucleotide primer; as well as kits for performing the method.

Abstract: A method of ligating DNA molecules, wherein the DNA molecules are in a hybrid with an RNA molecule, including the steps of providing DNA molecules that are in a RNA:DNA hybrid with an RNA molecule, and ligating the DNA molecules to each other with a double strand specific ligase

Abstract: The present invention relates to methods for generating an amplified nucleic acid portion of a template nucleic acid, comprising obtaining said template nucleic acid, annealing at least one oligonucleotide primer to said template nucleic acid, annealing at least one oligonucleotide stopper to said template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner until the elongating product nucleic acid reaches the position of an annealed oligonucleotide stopper, whereby the elongation reaction is stopped, wherein in said elongation reaction said oligonucleotide stopper is not elongated, and wherein the elongated product nucleic acid is ligated to the 3? end of said oligonucleotide stopper—said stopper may also be a primer itself and uses and kits for performing said method.