Abstract: Methods of infection, hold times and storage temperatures are provided to extend the viability and stabilize the phenotype of the cytolytic cells as a monotherapy and cytolytic cells containing an oncolytic virus capable of cell cycle arrest. Pre-infection of effector cells prior to storage at 4° C. or room temperature resulted in sustained viability of expanded immune cells and a higher percentage of CD3+/CD56+ T cells including a higher percentage of those CD3+/CD56+ T cells expressing the NKG2D receptor. In some embodiments, viral infection of immune cells increases the expression of the NKG2C, D and E receptors and influence the migration, binding and cytolytic activity towards tumor cells representing synergy between the immune cell and oncolytic virus in attacking and lysing tumor cells.

Abstract: Methods for mutagenizing and selecting microorganisms with increased product tolerance are provided. Additionally, methods and systems are disclosed for culturing the microorganisms to produce products.

Abstract: Compositions and methods are provided for enhanced expression of light emitting reporters. Such reporters are used in methods for monitoring cultures for production of target compounds.

Abstract: This invention provides compositions and methods for monitoring and regulating the production of a target product of a biochemical pathway in an organism, such as butanol. A gene encoding a light-emitting reporter molecule, such as luciferase, is operatively linked with a transcription regulatory nucleotide sequence that regulates transcription of an enzyme in the pathway that signals the rate of production of the target product, such as butanol dehydrogenase. When a microorganism is transfected with such a reporter construct and cultured, the reporter is expressed contemporaneously with the enzyme. The amount of light produced by the reporter indicates amount of enzyme being produced which, in turn, signals the amount of target product being produced. When the reporter is measured in real time, it provides information that can be used to regulate culture conditions and to optimize production of the target product.

Abstract: Compositions and methods are provided for enhanced expression of light emitting reporters. Such reporters are used in methods for monitoring cultures for production of target compounds. Additionally, methods are provided for the use of light emitting reporter systems and other reporter systems for selecting desired traits in an organism and for identifying or optimizing culture conditions.

Abstract: Methods for adapting or selecting microorganisms with increased product tolerance are provided Additionally, a bioreactor capable of operation in either packed bed or fluidized bed is disclosed along with methods to use the bioreactor for culturing microorganisms adapted or selected increased product tolerance

Abstract: Compositions and methods are provided for enhanced expression of light emitting reporters. Such reporters are used in methods for monitoring cultures for production of target compounds.

Abstract: This invention provides compositions and methods for monitoring and regulating the production of a target product of a biochemical pathway in an organism, such as butanol. A gene encoding a light-emitting reporter molecule, such as luciferase, is operatively linked with a transcription regulatory nucleotide sequence that regulates transcription of an enzyme in the pathway that signals the rate of production of the target product, such as butanol dehyrogenase. When a microorganism is transfected with such a reporter construct and cultured, the reporter is expressed contemporaneously with the enzyme. The amount of light produced by the reporter indicates amount of enzyme being produced which, in turn, signals the amount of target product being produced. When the reporter is measured in real time, it provides information that can be used to regulate culture conditions and to optimize production of the target product.

Abstract: The present invention relates to novel transcription control elements, including promoters, derived from angiogenesis-related genes, particularly the mouse VEGF gene, the mouse VEGFR-2 receptor gene, and the mouse Tie2 gene. Also disclosed are isolated polynucleotides comprising such promoters, as well as nucleic acid constructs comprising such promoters operatively linked to genes encoding a gene product, such as, a reporter, a protein, polypeptide, hormone, ribozyme, or antisense RNA, and to recombinant cells and transgenic animals comprising such nucleic acid constructs. The present invention further relates to screening methods using those recombinant cells and transgenic animals, particularly methods of screening for therapeutic compounds that modulate tumorigenesis and angiogenesis.

Abstract: The present invention relates to novel transcription control elements, including promoters, derived from angiogenesis-related genes, particularly the mouse VEGF gene, the mouse VEGFR-2 receptor gene, and the mouse Tie2 gene. Also disclosed are isolated polynucleotides comprising such promoters, as well as nucleic acid constructs comprising such promoters operatively linked to genes encoding a gene product, such as, a reporter, a protein, polypeptide, hormone, ribozyme, or antisense RNA, and to recombinant cells and transgenic animals comprising such nucleic acid constructs. The present invention further relates to screening methods using those recombinant cells and transgenic animals, particularly methods of screening for therapeutic compounds that modulate tumorigenesis and angiogenesis.