Patents by Inventor Pamela S. Vandivort

Pamela S. Vandivort has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Immunohistochemical staining method and reagents therefor
    Patent number: 5418138
    Abstract: The present invention provides an improved method for staining slides using immunochemical reagents. The method comprises the following steps. The assay region of a slide (the region containing the tissue section)is washed with an improved rinsing solution comprising water and a detergent. An evaporation inhibitor liquid is applied to the slide to cover the assay region. For antigens requiring unmasking, the tissue section is combined with an improved, stabilized proteolytic enzyme solution. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. A primary antibody in an improved diluent containing globulins from the same species as a second antibody is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied. A labeled second antibody in the improved diluent is combined with the tissue section for a time sufficient for substantially complete antibody binding.
    Type: Grant
    Filed: March 11, 1994
    Date of Patent: May 23, 1995
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Phillip C. Miller, Michael J. Degroff, Michael J. Gizinski, James A. Rybski, Pamela S. Vandivort, Anthony L. Hartman
  • Immunohistochemical staining method and reagents therefor
    Patent number: 5322771
    Abstract: The present invention provides an improved method for staining slides using immunochemical reagents. The method comprises the following steps. The assay region of a slide (the region containing the tissue section) is washed with an improved rinsing solution comprising water and a detergent. An evaporation inhibitor liquid is applied to the slide to cover the assay region. For antigens requiring unmasking, the tissue section is combined with an improved, stabilized proteolytic enzyme solution. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. A primary antibody in an improved diluent containing globulins from the same species as a second antibody is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied. A labeled second antibody in the improved diluent is combined with the tissue section for a time sufficient for substantially complete antibody binding.
    Type: Grant
    Filed: August 31, 1992
    Date of Patent: June 21, 1994
    Assignee: Ventana Medical Systems, Inc
    Inventors: James A. Rybski, Pamela S. Vandivort, Anthony L. Hartman, Phillip C. Miller, Michael J. Degroff, Michael J. Gizinski
  • Immunohistochemical staining method and reagents therefor
    Patent number: 5225325
    Abstract: The present invention provides an immunohistochemical staining method using improved reagents. In one aspect, the method uses an evaporation inhibitor liquid to prevent evaporation of reagents applied to an assay region of a slide.
    Type: Grant
    Filed: March 2, 1990
    Date of Patent: July 6, 1993
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Phillip C. Miller, Michael J. Degroff, Michael J. Gizinski, James A. Rybski, Pamela S. Vandivort