Abstract: The present disclosure discloses a recombinant microbe producing podophyllotoxin, or its derivatives, comprising genes encoding phenyl alanine ammonia-lyase (PAL), cinnamate-4-hydroxylate (C4H), 4-coumaroyl CoA-ligase (4CL), hydroxycinnamoyl-CoA quinate hydroxycinnamoyltransferase (HCT), p-coumaroyl quinate 3?-hydroxylase (C3H), caffeoyl CoA O-methyltransferase (CCoAOMT), bifunctional pinoresinol-lariciresinol reductase (DIRPLR), secoisolariciresinol dehydrogenase (SDH), cytochrome P450 oxidoreductase CYP719, O-methyltransferase (OMT), cytochrome P450 oxidoreductase CYP71, and 2-oxoglutarate/Fe(II)-dependent dioxygenase (2-ODD). Also disclosed herein is a method for producing podophyllotoxin or its derivatives. Moreover, a method of treating cancer is also disclosed.

Abstract: The present disclosure discloses a recombinant microbe producing podophyllotoxin, or its derivatives, comprising genes encoding phenyl alanine ammonia-lyase (PAL), cinnamate-4-hydroxylate (C4H), 4-coumaroyl CoA-ligase (4CL), hydroxycinnamoyl-CoA quinate hydroxycinnamoyltransferase (HCT), p-coumaroyl quinate 3?-hydroxylase (C3H), caffeoyl CoA O-methyltransferase (CCoAOMT), bifunctional pineresionl-lariciresinol reductase (DIRPLR), secoisolariciresinol dehydrogenase (SDH), cytochrome P450 oxidoreductase CYP719, O-methyltransferase (OMT), cytochrome P450 oxidoreductase CYP71, and 2-oxoglutarate/Fe(II)-dependent dioxygenase (2-ODD). Also disclosed herein is a method for producing podophyllotoxin or its derivatives. Moreover, a method of treating cancer is also disclosed.

Abstract: The present invention provides a process of preparing herbal filler composition for biri, cigarette and the like comprising: gradual sun drying leaves of Cichorium intybus (Chicory) till the moisture of leaves is reduced to 10% to 12%, removing the midribs from said leaves, shredding said leaves without midribs in a shredder to the desired size, 10-90% tobacco in the said shredded leaves, optionally, adding flavouring agents, as herein described, in said shredded leaves and tobacco, if desired and mixing evenly, and filing said flavoured shredded leaves in biri, cigarette and the like for smoking.

Abstract: The present invention defines a herbal composition for the treatment of chronic obstructive pulmonary disease, bronchitis and respiratory disorders and a process for preparing the same.

Abstract: This invention relates to a process of preparing storage stable aqueous Azadirachtin containing Azadirachtin A to provide a predominant pesticidal composition which comprises the steps of diluting 50,000-100,000 ppm Azadirachtin with 25,000 to 50,000 Azadirachtin A weight/volume with ethanol water solvent in a ratio in the range 100-70:0-30 volume/volume to produce 2,000-40,000 ppm Azadirachtin containing 1,000-20,000 ppm of Azadirachtin A weight/volume. This is followed by emulsifying of the diluted Azadirachtin with non-ionic and non-toxic emulsifying agents in an amount ranging from 0.2 to 30% and neem oil 20-50% volume/volume. The pH of the emulsified diluted Azadirachtin is adjusted to 3.5 to 6.0 by adding an alkali solution. 1-2.5% of a sunscreen like p.aminobenzoic acid or its esters and 1-10% oleic acid volume/volume are added to create a micro-emulsion for stabilizing the composition with a good bio-efficacy.