Abstract: The present disclosure relates to a method for preparing a waterborne polyurethane material, including the following acts: mixing polyester polyol and toluene diisocyanate, heating up to react, adding a mixed solution of dimethylolpropionic acid and butanediol to react, adding polyethylene glycol to react, adding trimethylolpropane to react, adding a catalyst to react and testing NCO value until reaching 1-3% of the theoretical value, adding a solvent and stirring uniformly to obtain a polyurethane prepolymer, cooling the prepolymer to <15° C., adding triethylamine for neutralization, adding pure water for thorough emulsification, adding ethylenediamine for chain extension, removing the solvent to obtain the waterborne polyurethane material. The present disclosure also relates to a method for preparing gloves, including: cleaning a mold, adding coagulants and demoulding agents, immersing in the waterborne polyurethane added with cross-linking agents, and then drying.