Abstract: The present invention discloses a illumination system for ophthalmic microscope and its operation method. Light beams generated by White LED, passes through the condenser and relay system to produce illumination on retina of the eye and boundary of the illumination is determined by the aperture stop. To enhance the visualization of red glow, a Single source beam then divided into two beams using divider system contains Beam splitter and mirror. Transmitted beams and reflected beams are made to focus on circular areas coated with reflective material of virtual beam splitter which is positioned coaxially with observation system of the microscope. Focused beam then passes through the objective lens of the microscopic system. Reflected beams from retina then passes through the objective lens and reaches the observation system via Virtual Beam Splitter.