Patents by Inventor Pascal Mayer
Pascal Mayer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20130164736Abstract: The present invention relates to a method for detecting molecular interactions in a solution. In particular, the present invention relates to a method for detecting interactions between two substances that are likely to interact with one another. The present invention can be used in particular in the field of scientific research and in the field of medical analysis.Type: ApplicationFiled: June 30, 2011Publication date: June 27, 2013Applicant: BIOFILM CONTROLInventors: Thierry Bernardi, Pascal Mayer, Jérôme Groelly
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Patent number: 8143008Abstract: A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilized primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provide further extended primers. The process can be repeated to provide amplified, immobilized nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc.Type: GrantFiled: April 30, 2010Date of Patent: March 27, 2012Assignee: Illumina, Inc.Inventors: Eric H. Kawashima, Laurent Farinelli, Pascal Mayer
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Patent number: 7985565Abstract: A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilized primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provided further extended primers. The process can be repeated to provide amplified, immobilized nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc.Type: GrantFiled: June 2, 2003Date of Patent: July 26, 2011Assignee: Illumina, Inc.Inventors: Pascal Mayer, Laurent Farinelli, Eric H. Kawashima
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Patent number: 7972820Abstract: Methods for isothermal amplification of nucleic acids by the means of a solid support are disclosed. These methods are useful for applications needing high throughput, in particular nucleic acid sequencing.Type: GrantFiled: May 5, 2010Date of Patent: July 5, 2011Assignees: Illumina Cambridge Limited, Illumina, Inc.Inventor: Pascal Mayer
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Publication number: 20110045541Abstract: A nucleic acid molecule can be annealed to an appropriate immobilised primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilised primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provide further extended primers. The process can be repeated to provide amplified, immobilised nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc.Type: ApplicationFiled: April 30, 2010Publication date: February 24, 2011Applicants: ILLUMINA CAMBRIDGE, LTD., ILLUMINA, INC.Inventors: Eric H. Kawashima, Laurent Farinelli, Pascal Mayer
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Publication number: 20110008781Abstract: Methods for isothermal amplification of nucleic acid by means of a solid support are disclosed. These methods are useful for applications needing high throughput, in particular nucleic acid sequencing.Type: ApplicationFiled: May 5, 2010Publication date: January 13, 2011Applicants: ILLUMINA CAMBRIDGE, LTD., ILLUMINA, INC.Inventor: Pascal Mayer
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Patent number: 7790418Abstract: Methods for isothermal amplification of nucleic acid by the means of a solid support are disclosed. These methods are useful for applications needing high throughput, in particular nucleic acid sequencing.Type: GrantFiled: December 7, 2001Date of Patent: September 7, 2010Assignees: Illumina Cambridge Limited, Illumina, Inc.Inventor: Pascal Mayer
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Publication number: 20080286795Abstract: A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilized primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provided further extended primers. The process can be repeated to provide amplified, immobilized nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc.Type: ApplicationFiled: April 15, 2008Publication date: November 20, 2008Applicant: Solexa LimitedInventors: Eric H. Kawashima, Laurent Farinelli, Pascal Mayer
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Publication number: 20080160580Abstract: Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5? end an oligonucleotide sequence Y and at the 3? end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5? end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5? end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5? ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally, performing at least one step of sequence determination ofType: ApplicationFiled: August 17, 2006Publication date: July 3, 2008Inventors: Celine Adessi, Eric Kawashima, Pascal Mayer, Jean-Jacques Mermod, Gerardo Turcatti
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Publication number: 20070015200Abstract: The invention provides methods for determining genome-wide sequence variations associated with a phenotype of a species in a hypothesis-free manner. In the methods of the invention, a set of restriction fragments for each of a sub-population of individuals having the phenotype are generated by digesting nucleic acids from the individual using one or more different restriction enzymes. A set of restriction sequence tags for the individual is then determined from the set of restriction fragments. The restriction sequence tags for the sub-population of organisms are compared and grouped into one or more groups, each of which comprising restriction sequence tags that comprise homologous sequences. The obtained one or more groups of restriction sequence tags identify the sequence variations associated with the phenotype. The methods of the invention can be used for, e.g., analysis of large numbers of sequence variants in many patient samples to identify subtle genetic risk factors.Type: ApplicationFiled: September 14, 2006Publication date: January 18, 2007Inventors: Pascal Mayer, Ilia Leviev, Magne Osteras, Laurent Farinelli
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Patent number: 7115400Abstract: Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) contains at the 5? end an oligonucleotide sequence Y and at the 3? end an oligonucleotide sequence Z and, in addition, the nucleic acid(s) carry at the 5? end a means for attaching the nucleic acid(s) to a solid support; (2) mixing said nucleic acid template(s) with one or more colony primers X, which can hybridize to the oligonucleotide sequence Z and carries at the 5? end a means for attaching the colony primers to a solid support, in the presence of a solid support so that the 5? ends of both the nucleic acid template and the colony primers bind to the solid support; (3) performing one or more nucleic acid amplification reactions on the bound template(s), so that nucleic acid colonies are generated and optionally,Type: GrantFiled: September 30, 1999Date of Patent: October 3, 2006Assignee: Solexa Ltd.Inventors: Celine Adessi, Eric Kawashima, Pascal Mayer, Jean-Jacques Mermod, Gerardo Turcatti
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Publication number: 20050100900Abstract: A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilized primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provided further extended primers. The process can be repeated to provide amplified, immobilized nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc.Type: ApplicationFiled: June 2, 2003Publication date: May 12, 2005Applicant: MANTEIA SAInventors: Eric Kawashima, Laurent Farinelli, Pascal Mayer
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Patent number: 6827830Abstract: An electrophoretic method for purifying a nucleic acid sample is disclosed. According to the method, the electrophoresis is effective to substantially reduce the concentration of contaminants relative to the concentration of desired nucleic acid in the nucleic acid sample, thereby producing a purified nucleic acid. In the method, the loading and recovery wells may be the same or different, and the electric fields may be DC or alternating.Type: GrantFiled: September 20, 2000Date of Patent: December 7, 2004Assignees: Applera Corporation, University of OttawaInventors: Gary Slater, J. William Efcavitch, Guy Drouin, Pascal Mayer, Jean Rousseau, Hong Yan Zhou, Claudia Chiesa, Robert Ruhfel, Roger O'Neill
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Publication number: 20040096853Abstract: Methods for isothermal amplification of nucleic acid by the means of a solid support are disclosed. These methods are useful for applications needing high throughput, in particular nucleic acids sequencing.Type: ApplicationFiled: November 3, 2003Publication date: May 20, 2004Inventor: Pascal Mayer
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Publication number: 20040002090Abstract: The invention provides methods for determining genome-wide sequence variations associated with a phenotype of a species in a hypothesis-free manner. In the methods of the invention, a set of restriction fragments for each of a sub-population of individuals having the phenotype are generated by digesting nucleic acids from the individual using one or more different restriction enzymes. A set of restriction sequence tags for the individual is then determined from the set of restriction fragments. The restriction sequence tags for the sub-population of organisms are compared and grouped into one or more groups, each of which comprising restriction sequence tags that comprise homologous sequences. The obtained one or more groups of restriction sequence tags identify the sequence variations associated with the phenotype. The methods of the invention can be used for, e.g., analysis of large numbers of sequence variants in many patient samples to identify subtle genetic risk factors.Type: ApplicationFiled: March 4, 2003Publication date: January 1, 2004Inventors: Pascal Mayer, Ilia Leviev, Magne Osteras, Laurent Farinelli
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Publication number: 20030206832Abstract: The microfluidic dispensing device includes a patterned seal having a specified arrangement of holes and groves, and an connection plate having passages corresponding to the holes in the patterned seal. The patterned seal and the connection plate are configured to stack onto a planar substrate, such as a glass slide, and are sealed together and against the substrate by a negative pressure, or vacuum, applied through the connection plate. When the patterned seal is secured against the substrate, the grooves in the patterned seal form a network of channels for delivering fluids to specified regions on the substrate. The connection plate preferably includes one or more connectors for attaching fluid and/or vacuum lines, and optionally includes one or more sensors to monitor operation of the device. The system of the present invention also includes a device for automatically assembling and handling the microfluidic dispensing device.Type: ApplicationFiled: May 2, 2002Publication date: November 6, 2003Inventors: Pierre Thiebaud, Pascal Mayer, Eric Chevalier
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Patent number: 6610186Abstract: The invention concerns a method for separating particles or molecules whereby these particles or molecules are introduced in a separating medium and a moving force is applied to them in said medium. The method is characterised in that the separating medium is a ferrofluid, i.e. a colloidal suspension of magnetic particles and a magnetic field is applied to this ferrofluid generating therein at least an alternation of a zone rich in magnetic particles and a zone poor in magnetic particles, part at least of the region of the ferrofluid in which this alternation is generated is passed through by the particles or molecules to be separated during their migration.Type: GrantFiled: August 30, 1999Date of Patent: August 26, 2003Assignee: Centre National de la Recherche Scientifique (CNRS)Inventors: Pascal Mayer, Jérôme Bibette, Jean-Louis Viovy
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Publication number: 20020055100Abstract: Different nucleic acid molecules present at different locations can be sequenced in parallel Primers that are annealed to the nucleic acid molecules can be provided. Each location can then be provided with a nucleic acid polymerase and a nucleotide. It can then be determined whether or not the nucleotide has been used in primer extension and the process can be repeated. As an alternative to using primers, a nick in a double stranded nucleic acid molecule can provide a 3′-OH group for chain extension.Type: ApplicationFiled: September 30, 1999Publication date: May 9, 2002Inventors: ERIC H. KAWASHIMA, LAURENT FARINELLI, PASCAL MAYER
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Patent number: 6146511Abstract: An electrophoretic method for purifying a nucleic acid sample is disclosed. The method generally comprises the steps of (1) providing a nucleic acid sample comprising a desired nucleic acid and one or more contaminants, (2) providing an electrophoresis matrix having a loading well and a recovery well formed therein, (3) placing the nucleic acid sample into the loading well, (4) performing a first electrophoresis comprising electrophoresing the nucleic acid sample for a first time effective to transport the desired nucleic acid out of the loading well and into the electrophoresis matrix; and (5) performing a second electrophoresis comprising electrophoresing the nucleic acid sample for a second time effective to transport the desired nucleic acid out of the electrophoresis matrix and into the recovery well.Type: GrantFiled: January 30, 1998Date of Patent: November 14, 2000Assignees: The Perkin-Elmer Corporation, The University of OttawaInventors: Gary Slater, J. William Efcavitch, Guy Drouin, Pascal Mayer, Jean Rousseau, Hong Yan Zhou, Claudia Chiesa, Robert Ruhfel, Roger O'Neill