Abstract: The invention concerns a general method for the specific determination of DNA sequences by means of parallel amplification through nested polymerase chain reaction in a combined liquid-phase/solid-phase-DNA-microarray system, together with modifications derived therefrom to determine, for example, point mutations and to sequence DNA sub-regions, which according to a particular embodiment can also be unknown.

Abstract: The invention concerns a general method for the specific determination of DNA sequences by means of parallel amplification through nested polymerase chain reaction in a combined liquid-phase/solid-phase-DNA-microarray system, together with modifications derived therefrom to determine, for example, point mutations and to sequence DNA sub-regions, which according to a particular embodiment can also be unknown.

Abstract: A device with one or more flow through reaction chambers for investigation of one or more probes on a sensor chip. The device has (a) a sensor chip (b) a number of probe arrays corresponding to a number of samples to be investigated, arranged on the sensor chip spatially separated from one another, (c) an upper portion (d) a number of spatially separated chambers disposed in the upper portion corresponding in number to the number of probe arrays. The chambers are each provided with an inlet opening and an outlet opening. The position and dimension of the probe arrays and the chambers is relatively determined so that, after a sandwich-like combination of the sensor chip with the upper portion a respective precise fit reaction chamber is formed over each probe array with an inlet opening and an outlet opening.