Abstract: The present invention comprises method of detecting biomolecules in situ in gel separation medium following electrophoresis that provides sensitivity similar to blotting. This method is applicable to in situ detection of a wide range of biomolecules including proteins and nucleic acids. After electrophoretic separation of a sample in gel separation media comprising a gellable polymeric material other than cross-linked polyacrylamide, the gel is contacted with a solution comprising at least one detectably labeled reagent directed to a biomolecule under conditions suitable for binding of the reagent to the biomolecule. Alternatively, the gel is contacted with a solution comprising at least one non-detectably labeled reagent directed to a biomolecule and a solution comprising at least on detectably labeled reagent directed to a biomolecule. In either case, binding of the detectably labeled reagent is assessed and indicates detection of biomolecules in the gel.

Abstract: The present invention provides various electrophoretic apparatuses for discontinuous gel electrophoresis. One apparatus comprises a vessel and a removable gel cassette for holding the gel, the cassette divided into two separate compartments, each in fluid communication with a buffer reservoir. Access holes provided on the top of the cassette allow sample to be added to the gel inside the cassette. The cassette also optionally includes a gel viewing window and sample loading window. Another apparatus comprises a vessel with a raised platform in the middle on which the gel is placed and over which a gel restraining frame, generally rectangular in shape, is secured close to the gel surface. The frame when in place acts as a barrier to movement of electrode buffer between reservoirs. The frame and an open interior for buffer or fluid, which assists in gel cooling during electrophoresis.