Abstract: Galectin-1 polypeptide variants that include a mutation of the histidine residue corresponding to position 52 of the full-length amino acid sequence of native human Gal-1 as shown in SEQ ID NO: 1, the mutation being a substitution of the histidine to tyrosine or asparagine, providing resistance to acidosis otherwise resulting in deactivation of the native human Gal-1. The Galectin-1 polypeptide variants may include one or more additional mutation(s) of the cysteine residue corresponding to a position selected from 2, 16, 88, or combinations thereof of the full-length amino acid sequence of native human Gal-1 as shown in SEQ ID NO: 1, the additional mutation being a substitution of the cysteine to serine, and providing resistance to oxidation.

Abstract: Galectin-1 polypeptide variants that include a mutation of the histidine residue corresponding to position 52 of the full-length amino acid sequence of native human Gal-1 as shown in SEQ ID NO: 1, the mutation being a substitution of the histidine to tyrosine or asparagine, providing resistance to acidosis otherwise resulting in deactivation of the native human Gal-1. The Galectin-1 polypeptide variants may include one or more additional mutation(s) of the cysteine residue corresponding to a position selected from 2, 16, 88, or combinations thereof of the full-length amino acid sequence of native human Gal-1 as shown in SEQ ID NO: 1, the additional mutation being a substitution of the cysteine to serine, and providing resistance to oxidation.

Abstract: Methods and apparatus for gas metal arc welding are provided. The methods of the invention allow production of high quality welds using shielding gas atmospheres having at least 30% carbon dioxide and electrode diameters less than 1.6 mm. The metal transfer mode is not short-circuit transfer. The invention also provides welding torch contact tips having a localized contact region.

Abstract: Electron beam welding of a thin layer to a substrate is accomplished using controlled volumetric heating of the respective substrate layers, thus minimizing gradients due to heat conduction. Electron beam penetration of the layers and velocity across the surface creates a rapidly translating weld pool within the substrates. The control parameters for the electron beam source are dependent on the heat characteristics of the substrate materials, their thickness, available electron beam power and speed, and desired finished weld geometry. The Peclet number maintained during the process is greater than about 1 and less than about 10.