Patents by Inventor Patrick A. Limbach

Patrick A. Limbach has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Compositions, methods, and kits for one-step digestion of nucleic acid for analysis by liquid chromatography tandem mass spectrometry
    Patent number: 11441170
    Abstract: A kit for preparing a nucleic acid sample for analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) is provided, the kit comprising: a lyophilized enzyme composition comprising: micrococcal nuclease; nuclease P1; and bacterial alkaline phosphatase (BAP); and a digestion buffer. Also provided are enzyme compositions and methods of use for rapid, efficient preparation of a nucleic acid sample for analysis by LC-MS/MS, without the need for denaturation of the sample.
    Type: Grant
    Filed: October 26, 2018
    Date of Patent: September 13, 2022
    Assignee: University of Cincinnati
    Inventors: Robert Ross, Patrick A. Limbach, Balasubrahmanyam Addepalli, Manasses Jora
  • Compositions, Methods, and Kits for One-Step Digestion of Nucleic Acid for Analysis by Liquid Chromatography Tandem Mass Spectrometry
    Publication number: 20200255886
    Abstract: A kit for preparing a nucleic acid sample for analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) is provided, the kit comprising: a lyophilized enzyme composition comprising: micrococcal nuclease; nuclease P1; and bacterial alkaline phosphatase (BAP); and a digestion buffer. Also provided are enzyme compositions and methods of use for rapid, efficient preparation of a nucleic acid sample for analysis by LC-MS/MS, without the need for denaturation of the sample.
    Type: Application
    Filed: October 26, 2018
    Publication date: August 13, 2020
    Inventors: Robert Ross, Patrick A. Limbach, Balasubrahmanyam Addepalli, Manasses Jora