Patents by Inventor Patrick Alan Limbach

Patrick Alan Limbach has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Recombinant nucleoside-specific ribonuclease and method of producing and using same
    Patent number: 11535834
    Abstract: A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MC1 and a cytidine-specific recombinant RNase Cusativin.
    Type: Grant
    Filed: November 12, 2019
    Date of Patent: December 27, 2022
    Assignee: University of Cincinnati
    Inventors: Sarah Venus, Balasubrahmanyam Addepalli, Nicholas Paul Lesner, Patrick Alan Limbach
  • RECOMBINANT NUCLEOSIDE-SPECIFIC RIBONUCLEASE AND METHOD OF PRODUCING AND USING SAME
    Publication number: 20200115689
    Abstract: A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MC1 and a cytidine-specific recombinant RNase Cusativin.
    Type: Application
    Filed: November 12, 2019
    Publication date: April 16, 2020
    Inventors: Sarah Venus, Balasubrahmanyam Addepalli, Nicholas Paul Lesner, Patrick Alan Limbach
  • Recombinant nucleoside-specific ribonuclease and method of producing and using same
    Patent number: 10533163
    Abstract: A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MC1 and a cytidine-specific recombinant RNase Cusativin.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: January 14, 2020
    Assignee: University of Cincinnati
    Inventors: Sarah Venus, Balasubrahmanyam Addepalli, Nicholas Paul Lesner, Patrick Alan Limbach
  • RECOMBINANT NUCLEOSIDE-SPECIFIC RIBONUCLEASE AND METHOD OF PRODUCING AND USING SAME
    Publication number: 20180320154
    Abstract: A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MC1 and a cytidine-specific recombinant RNase Cusativin.
    Type: Application
    Filed: April 25, 2016
    Publication date: November 8, 2018
    Inventors: Sarah Venus, Balasubrahmanyam Addepalli, Nicholas Paul Lesner, Patrick Alan Limbach