Patents by Inventor Patrick C. Cirino

Patrick C. Cirino has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Peroxide-driven cytochrome P450 oxygenase variants
    Patent number: 7704715
    Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T, L324I, I366V, K434E, E442K, and/or V446I amino acid substitutions.
    Type: Grant
    Filed: October 11, 2008
    Date of Patent: April 27, 2010
    Assignee: California Institute of Technology
    Inventors: Patrick C. Cirino, Frances H. Arnold
  • Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use
    Publication number: 20090264311
    Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have at least one mutation improving their ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. The variants also have at least one mutation improving thermostability as compared to the parent enzyme or corresponding wild-type enzyme. Preferred variants include cytochrome P450 BM-3 heme domain variants having L52I, I58V, F87A, H100R, S106R, F107L, A135S, M145A/V, A184V, N239H, S274T, L324I, V340M, I366V, K434E, E442K, and/or V446I amino acid substitutions.
    Type: Application
    Filed: October 11, 2008
    Publication date: October 22, 2009
    Applicant: The California Institute of Technology
    Inventors: Frances H. Arnold, Patrick C. Cirino
  • Peroxide-driven cytochrome P450 oxygenase variants
    Publication number: 20090142821
    Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having 158V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T, L3241, I366V, K434E, E442K, and/or V446I amino acid substitutions.
    Type: Application
    Filed: October 11, 2008
    Publication date: June 4, 2009
    Applicant: The California Institute of Technology
    Inventors: Patrick C. Cirino, Frances H. Arnold
  • Peroxide-driven cytochrome P450 oxygenase variants
    Patent number: 7465567
    Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T, L324I, I366V, K434E, E442K, and/or V446I amino acid substitutions.
    Type: Grant
    Filed: April 16, 2002
    Date of Patent: December 16, 2008
    Assignee: California Institute of Technology
    Inventors: Patrick C. Cirino, Frances H. Arnold
  • Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use
    Patent number: 7435570
    Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have at least one mutation improving their ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. The variants also have at least one mutation improving thermostability as compared to the parent enzyme or corresponding wild-type enzyme. Preferred variants include cytochrome P450 BM-3 heme domain variants having L52I, I58V, F87A, H100R, S106R, F107L, A135S, M145A/V, A184V, N239H, S274T, L324I, V340M, I366V, K434E, E442K, and/or V446I amino acid substitutions.
    Type: Grant
    Filed: August 11, 2004
    Date of Patent: October 14, 2008
    Assignee: California Institute of Technology
    Inventors: Frances H. Arnold, Patrick C. Cirino