Abstract: Systems, methods, and compositions for identifying genomic variants and methylation analysis, including synthetic polynucleotide libraries, are provided. The synthetic polynucleotide libraries may comprise a plurality of polynucleotides. The polynucleotides may comprise sequences corresponding to a genetic abnormality in a genome. The stoichiometry of each of the plurality of polynucleotides is controlled. Systems, methods, and compositions described herein may include standards for determining the analytical sensitivity and/or accuracy of instruments configured to measure nucleic acid variant frequencies. Standards may comprise RNA-fusions and/or CNV mutations related to cancer.

Abstract: Provided herein are methods, systems, and compositions for efficient nucleic acid assembly with improved representation and distribution. Provided herein are methods, systems, and compositions for efficient nucleic acid assembly of nucleic acids encoding genes for use in various downstream processes.

Abstract: Provided herein are compositions and methods for identifying genomic variants. Further provided herein are standards useful for determining the analytical sensitivity and/or accuracy of instruments configured to measure nucleic acid variant frequencies.

Abstract: A composite plectrum with a strum direction is disclosed. The plectrum is generally triangular in shape and includes a first layer of wood having a first grain direction, a layer of silk fabric with silk threads that are woven at angles to one another, and a second layer of wood with a grain in a second grain direction. The second layer of wood being adhered to the layer of silk while at 90 degrees to the first layer of wood, so that the layer of silk extends between the two layers of wood and is adhered to both layers of wood.