Abstract: Embodiments described herein relate to a tool comprising a cylindrical member having a cylindrical tube portion with a circular opening at a first end of the cylindrical member, and a protrusion disposed on an inner surface of the cylindrical tube portion. In various embodiments, the tool includes a plurality of protrusions that are disposed equal-distant from one another along a circumference on the inner surface of the cylindrical tube portion. In various embodiments, the cylindrical member comprises a second cylindrical tube portion with a second circular opening on a second end of the cylindrical member. In various embodiments, the protrusion has a varying height that gradually increases from a first end of the protrusion to a second end. In various embodiments, the protrusion is disposed at a slanted angle with respect to the circular opening where the first end is closer to the circular opening than the second end.

Abstract: Provided herein are systems, devices and methods for harvesting biological cells with a centrifuge. A biological cell harvesting centrifuge apparatus may be provided with a rectangularly shaped base and 21 laterally spaced apart upright supports. Each upright support may extend vertically upward from the base and have a hollow core. Each upright support may be configured to slidably receive and support either one, two or four vertical edges of either one, two or four 15 mL micro bioreactor vessels. The 21 upright supports may be arranged in a 3×7 array such that they form a 2×6 array of receptacles configured to slidably receive and support 12 of the bioreactor vessels. The upright supports may be configured to support at least 80% of the vertical edges of the bioreactor vessels.

Abstract: The presently disclosed subject matter relates to cell culture media and cell culture strategies for modulating the glycosylation pattern, e.g., fucosylation and/or galactosylation pattern, of a glycoprotein of interest, e.g., an antibody, as well as cell culture and glycoprotein compositions prepared using such media and/or strategies.