Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

Abstract: The present invention relates to peptides that are derived from HSV-2 glycoprotein G2 and represent HSV-2 type-specific epitopes. The present invention also provides for compositions comprising these peptides for type-specific serological diagnosis of HSV-2 infection. Methods of using these peptides for type-specific detection of HSV-2 antibodies and differentiation of HSV-2 viral infection from HSV-1 and other herpes family viral infections are further provided.

Abstract: Novel polypeptides are provided having substantially the same sequence as immunologically significant fragments of AIDS-related viruses. The polypeptides can be used as reagents in the determination of exposure of a human host to the virus. Of particular interest is the use of polypeptides in screening blood products.

Abstract: The present invention relates to peptides that are derived from HSV-2 glycoprotein G2 and represent HSV-2 type-specific epitopes. The present invention also provides for compositions comprising these peptides for type-specific serological diagnosis of HSV-2 infection. Methods of using these peptides for type-specific detection of HSV-2 antibodies and differentiation of HSV-2 viral infection from HSV-1 and other herpes family viral infections are further provided.

Abstract: Peptides immunoreactive with antibodies to native proteins, and which have at least two cysteine residues that contribute to mimicking an epitope of the protein, are prepared with the cysteine thiol groups protected. When deprotected, the peptides have enhanced immunoreactivity.

Abstract: Peptides immunoreactive with antibodies to native proteins, and which have at least two cysteine residues that contribute to mimicking an epitope of the protein, are prepared with the cysteine thiol groups protected. When deprotected, the peptides have enhanced immunoreactivity. The peptides are particularly useful for detecting antibodies or antigens associated with retroviruses, including the clinically important lymphotropic retroviruses HIV-1, HIV-2, HTLV-I, and HTLV-II.

Abstract: A two part peroxide/chromogen reagent for use in peroxidase labeled binding assays which is stable for at least about 24 hours is disclosed. The reagent is provided as two components, the first component preferably incorporating a peroxide dissolved in a first solvent, and the second component preferably having a chromogenic substance dissolved in a second solvent where the two components can be mixed within about 48 hours of colorimetric detection of an enzyme immunoassay.

Abstract: Methods and composition are provided for diagnostic assays, where a sample traverses a bibulous matrix in a first plane and the flow path redirected into a second plane at a binding site, where a signal is created in relation to the presence of analyte. Various protocols may be employed where components of a signal producing system may be combined with the sample, the matrix, or added at the binding site.