Abstract: The present invention provides for a nucleic acid encoding refactored minimized set of Agrobacterium virulence genes. The present invention provides for a method for introducing a nucleic acid of interest into a eukaryotic cell, the method comprises: (a) providing (i) a first nucleic acid encoding a refactored minimized set of Agrobacterium virulence genes operatively linked to one or more promoters; and (ii) a second nucleic acid comprising a nucleic acid of interest flanked by a left border and a right border; (b) introducing the first nucleic acid and the second nucleic acid into a target host cell; and, (c) the nucleic acid of interest is stably integrated into a genome of the target host cell.

Abstract: The present invention provides for a synthetic transcription factor (TF) comprising (a) a DNA-binding domain of a transcription factor linked to (b) an activator domain (AD), and (c) optionally a nuclear localization sequence (NLS). The present invention provides for a nucleic acid encoding an activator domain (AD) of the present invention.

Abstract: Mammalian milk oligosaccharides (MMO) are produced in plants engineered to express recombinant MMO biosynthetic pathways.

Abstract: The present invention provides for a genetically modified plant or plant cell comprising a nucleic acid encoding one or more heterologous enzymes operatively linked a promoter, wherein one or more heterologous enzymes synthesizes muconic acid (MA).

Abstract: The present invention provides for a synthetic transcription factor (TF) comprising (a) a DNA-binding domain of a transcription factor linked to (b) an effector domain, and (c) optionanlly a nuclear localization sequence (NLS). The present invention provides for a nucleic acid encoding an effector domain of the present invention. The DNA-binding domain can be a deactivated RNA-guided nuclease variant of Cas9 (dCas9).

Abstract: The present invention provides for a synthetic transcription factor (TF) comprising (a) a DNA-binding domain of a transcription factor linked to (b) an activator domain or repressor domain, and (c) a nuclear localization sequence (NLS).

Abstract: The present invention provides for a genetically modified plant or plant cell comprising a nucleic acid encoding one or more heterologous enzymes operatively linked a promoter, wherein one or more heterologous enzymes synthesizes muconic acid (MA).

Abstract: The present invention provides for a genetically modified plant cell or plant, comprising: (a) (i) one or more nucleic acids each encoding one or more transcription factors (or transcription activators) operatively linked to a first tissue-specific or inducible promoter, (ii) one or more nucleic acids each encoding one or more transcription repressors each operatively linked to a second tissue-specific or inducible promoter, or (iii) combinations thereof; and (b) one or more nucleic acids each encoding one or more independent genes of interest (GOI) each operatively linked to a promoter that is activated by the one or more transcription factors (or transcription activators), repressed by the one or more transcription repressors, or a combination of both.