Patents by Inventor Patrick McCoy Spooner
Patrick McCoy Spooner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11834670Abstract: A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5? flanking sequence and a donor 3? flanking sequence. The donor 5? flanking sequence and the donor 3? flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.Type: GrantFiled: April 19, 2017Date of Patent: December 5, 2023Assignee: GLOBAL LIFE SCIENCES SOLUTIONS USA LLCInventors: John Richard Nelson, Robert Scott Duthie, Patrick McCoy Spooner, John Anthony Schiel, Lisa Anne Lowery, Anja Josifa Smith
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Patent number: 11033859Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.Type: GrantFiled: June 19, 2018Date of Patent: June 15, 2021Assignee: Global Life Sciences Solutions Operations UK LtdInventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk, Nicole Lea Wood, Li Zhu, Craig Patrick Galligan
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Patent number: 10598577Abstract: A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.Type: GrantFiled: June 30, 2017Date of Patent: March 24, 2020Assignee: General Electric CompanyInventors: Arunkumar Natarajan, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk, Wei Gao, Kwok Pong Chan, Lakshmi Sireesha Kaanumalle, Abraham Joy, Nicholas Nun
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Patent number: 10472620Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.Type: GrantFiled: July 1, 2014Date of Patent: November 12, 2019Assignee: General Electric CompanyInventors: John Richard Nelson, David Roger Moore, Robert Scott Duthie, Matthew Jeremiah Misner, Gregory Andrew Grossmann, Elizabeth Marie Dees, Patrick McCoy Spooner, Erik Leeming Kvam, Andrew Arthur Paul Burns, Vicki Herzl Watkins
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Publication number: 20190003940Abstract: A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.Type: ApplicationFiled: June 30, 2017Publication date: January 3, 2019Inventors: Arunkumar Natarajan, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk, Wei Gao, Kwok Pong Chan, Lakshmi Sireesha Kaanumalle, Abraham Joy, Nicholas Nun
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Publication number: 20180361317Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.Type: ApplicationFiled: June 19, 2018Publication date: December 20, 2018Inventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk, Nicole Lea Wood, Li Zhu, Craig Patrick Galligan
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Publication number: 20180327811Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific binding element. This results in the protamine-specific binding element binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.Type: ApplicationFiled: July 23, 2018Publication date: November 15, 2018Inventors: Patrick McCoy SPOONER, Peter James TATNELL, Jeffrey Kenneth HORTON, John Richard NELSON, Michael John GERDES, Suzana KIEL, Ralf LENIGK, Alexander SCHENK, Wei SUN, Thomas HANSEN
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Publication number: 20180305718Abstract: A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5? flanking sequence and a donor 3? flanking sequence. The donor 5? flanking sequence and the donor 3? flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.Type: ApplicationFiled: April 19, 2017Publication date: October 25, 2018Inventors: John Richard Nelson, Robert Scott Duthie, Patrick McCoy Spooner, John Anthony Schiel, Lisa Anne Lowery, Anja Josifa Smith
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Patent number: 10030241Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.Type: GrantFiled: May 10, 2016Date of Patent: July 24, 2018Assignee: GENERAL ELECTRIC COMPANYInventors: Patrick McCoy Spooner, Peter James Tatnell, Jeffrey Kenneth Horton, John Richard Nelson, Michael John Gerdes, Suzana Kiel, Ralf Lenigk, Alexander Schenk, Wei Sun
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Patent number: 9999856Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.Type: GrantFiled: July 26, 2013Date of Patent: June 19, 2018Assignee: General Electric CompanyInventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk, Nichole Lea Wood, Li Zhu, Craig Patrick Galligan
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Patent number: 9957548Abstract: A method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample is disclosed. The method includes a step of contacting a lysis solution to the sample that includes at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate includes a protamine-DNA complex. The method further includes applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The method further includes capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.Type: GrantFiled: March 30, 2015Date of Patent: May 1, 2018Assignee: GENERAL ELECTRIC COMPANYInventors: Michael John Gerdes, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk
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Patent number: 9593368Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; applying an aqueous buffer to the sample application zone of the substrate to washes away one or more inhibitors present on the sample application zone; and applying an isothermal nucleic acid amplification reaction mixture to the sample application zone to amplify the target nucleic acid to form a nucleic acid amplification product. The target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight.Type: GrantFiled: July 1, 2014Date of Patent: March 14, 2017Assignee: General Electric CompanyInventors: John Richard Nelson, Robert Scott Duthie, Christopher Michael Puleo, Patrick McCoy Spooner
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Publication number: 20160289666Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.Type: ApplicationFiled: May 10, 2016Publication date: October 6, 2016Inventors: Patrick McCoy Spooner, Peter James Tatnell, Jeffrey Kenneth Horton, John Richard Nelson, Michael John Gerdes, Suzana Kiel, Ralf Lenigk, Alexander Schenk, Wei Sun
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Publication number: 20160289735Abstract: A method is provided herein, wherein the method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample, comprises contacting a lysis solution to the sample comprising at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate comprises a protamine-DNA complex. The method further comprises applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The antibody binding is followed by capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.Type: ApplicationFiled: March 30, 2015Publication date: October 6, 2016Inventors: Michael John Gerdes, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk
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Patent number: 9399986Abstract: A device, a system, and a method for isolating biomolecules from biological materials are provided. The device comprises a quartz-based solid phase extraction matrix comprising one or more reagents impregnated therein; and an electroosmotic pump (EOP) operationally coupled to the quartz-based solid phase extraction matrix to elute the nucleic acids, wherein the EOP comprises a plurality of electroosmotic membranes comprising one or more positive electroosmotic membranes and one or more negative electroosmotic membranes disposed alternatively and a plurality of electrodes comprising one or more cathodes and one or more anodes, wherein at least one cathode is disposed on one side of one of the membranes and at least one anode is disposed on another side of that membrane and at least one cathode or anode is disposed between a positive electroosmotic membrane and a negative electroosmotic membrane.Type: GrantFiled: August 30, 2012Date of Patent: July 26, 2016Assignee: General Electric CompanyInventors: John Richard Nelson, Li Zhu, Xiaohui Chen, Christopher Michael Puleo, Patrick McCoy Spooner
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Patent number: 9333463Abstract: A device and a system for eluting biomolecules from biological sample by electroelution are provided. The device for electroelution of biomolecules from a biological sample is constituted with a housing configured to receive an electrolyte and the biological sample, at least two electrodes comprising conductive redox polymers operationally coupled to the housing, and a biomolecule impermeable layer disposed on at least one of the electrodes. The biomolecule impermeable layer disposed on at least one of the electrodes to prevent the biomolecules from reaching the electrode. A system is provided, wherein the system comprises a sample collection port, one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof, an device for electroelution, and a controller.Type: GrantFiled: July 26, 2013Date of Patent: May 10, 2016Assignee: General Electric CompanyInventors: Christopher Michael Puleo, John Richard Nelson, Patrick McCoy Spooner, Ralf Lenigk, Nichole Lea Wood, Li Zhu, Craig Patrick Galligan
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Publication number: 20160123926Abstract: A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb.Type: ApplicationFiled: September 25, 2015Publication date: May 5, 2016Inventors: John Richard Nelson, Christopher Michael Puleo, Erin Jean Finehout, Patrick McCoy Spooner, Kashan Ali Shaikh, Xiaohui Chen, Li Zhu
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Publication number: 20160002621Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.Type: ApplicationFiled: July 1, 2014Publication date: January 7, 2016Inventors: John Richard Nelson, David Roger Moore, Robert Scott Duthie, Matthew Jeremiah Misner, Gregory Andrew Grossmann, Elizabeth Marie Dees, Patrick McCoy Spooner, Erik Leeming Kvam, Andrew Arthur Paul Burns, Vicki Herzl Watkins
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Publication number: 20160002715Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; applying an aqueous buffer to the sample application zone of the substrate to washes away one or more inhibitors present on the sample application zone; and applying an isothermal nucleic acid amplification reaction mixture to the sample application zone to amplify the target nucleic acid to form a nucleic acid amplification product. The target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight.Type: ApplicationFiled: July 1, 2014Publication date: January 7, 2016Inventors: John Richard Nelson, Robert Scott Duthie, Christopher Michael Puleo, Patrick McCoy Spooner
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Publication number: 20150285720Abstract: Disclosed are novel cold fixation methods and reagents comprising contacting a biological sample with a reagent for fixation at a temperature of less than 20° C. where the reagent comprises an aqueous buffer, a water soluble alkylnitrile, C2 to C6 alkyl ester, or combination thereof, and formaldehyde to the aqueous buffer solution and removing the biological sample from contact with the reagent.Type: ApplicationFiled: April 8, 2014Publication date: October 8, 2015Applicant: GENERAL ELECTRIC COMPANYInventors: Bruce Allan Hay, Brian James Grimmond, Lisa Anne Lowery, Qing Li, Patrick McCoy Spooner, Christine Angela Morton