Patents by Inventor Pau-Miau Yuan
Pau-Miau Yuan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20140179901Abstract: Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis.Type: ApplicationFiled: December 11, 2013Publication date: June 26, 2014Applicant: APPLIED BIOSYSTEMS, LLCInventors: Xiongwei YAN, Sheri Miraglia, Pau Miau Yuan
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Patent number: 8623995Abstract: Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis.Type: GrantFiled: June 14, 2010Date of Patent: January 7, 2014Assignee: Applied Biosystems, LLCInventors: Xiongwei Yan, Sheri Miraglia, Pau Miau Yuan
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Patent number: 8501498Abstract: This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.Type: GrantFiled: November 13, 2009Date of Patent: August 6, 2013Assignee: DH Technologies Development Pte. Ltd.Inventors: Xiongwei Yan, Pau-Miau Yuan, Sylvia W. Yuen, Kuo-Liang Hsi, Joe Y. Lam, Krishna G. Upadhya, Subhakar Dey, Darryl J. C. Pappin, Sasi Pillai, Helena Huang, Subhasish Purkayastha
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Patent number: 8003767Abstract: Fluorescent, sulfonated 3,7-diamino-[8,9]benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a variety of contexts, including cell surface assays employing intact, live cells and in nucleic acid detection methods. The new dyes are water soluble and can be conjugated to a variety of substrates, such as polynucleotides, nucleosides, nucleotides, peptides, proteins, antibodies, carbohydrates, ligands, particles and surfaces.Type: GrantFiled: June 29, 2007Date of Patent: August 23, 2011Assignee: Applied Biosystems, LLCInventors: Xiongwei Yan, Pau Miau Yuan
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Publication number: 20110097758Abstract: Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis.Type: ApplicationFiled: June 14, 2010Publication date: April 28, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Xiongwei Yan, Sheri Miraglia, Pau Miau Yuan
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Publication number: 20100311175Abstract: This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.Type: ApplicationFiled: November 13, 2009Publication date: December 9, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Xiongwei YAN, Pau Miau Yuan, Sylvia W. Yuen, Kuo-Liang Hsi, Joe Y. Lam, Krishna G. Upadhya, Subhakar Dey, Darryl J.C. Pappin, Sasi Pillai, Helena Huang, Subhasish Purkayastha
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Publication number: 20100273943Abstract: Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis.Type: ApplicationFiled: July 6, 2010Publication date: October 28, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Xiongwei YAN, Sheri Miraglia, Pau Miau Yuan
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Publication number: 20090004653Abstract: Fluorescent, sulfonated 3,7-diamino-[8,9]benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a variety of contexts, including cell surface assays employing intact, live cells and in nucleic acid detection methods. The new dyes are water soluble and can be conjugated to a variety of substrates, such as polynucleotides, nucleosides, nucleotides, peptides, proteins, antibodies, carbohydrates, ligands, particles and surfaces.Type: ApplicationFiled: June 29, 2007Publication date: January 1, 2009Inventors: Xiongwei Yan, Pau Miau Yuan
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Patent number: 7238789Abstract: Fluorescent, sulfonated 3,7-diamino-[8,9]benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a variety of contexts, including cell surface assays employing intact, live cells and in nucleic acid detection methods. The new dyes are water soluble and can be conjugated to a variety of substrates, such as polynucleotides, nucleosides, nucleotides, peptides, proteins, antibodies, carbohydrates, ligands, particles and surfaces.Type: GrantFiled: October 8, 2002Date of Patent: July 3, 2007Assignee: Applera CorporationInventors: Xiongwei Yan, Pau Miau Yuan
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Publication number: 20070048752Abstract: This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.Type: ApplicationFiled: February 15, 2006Publication date: March 1, 2007Applicant: Applera CorporationInventors: Xiongwei Yan, Pau-Miau Yuan, Sylvia Yuen, Kuo-Liang Hsi, Joe Lam, Krishna Upadhya, Subhakar Dey, Darryl Pappin, Sasi Pillai, Helena Huang, Subhasish Purkayastha
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Publication number: 20070009440Abstract: Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis.Type: ApplicationFiled: June 29, 2006Publication date: January 11, 2007Applicant: APPLERA CORPORATIONInventors: Xiongwei Yan, Sheri Miraglia, Pau Miau Yuan
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Publication number: 20060172319Abstract: This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.Type: ApplicationFiled: July 11, 2005Publication date: August 3, 2006Applicant: Applera CorporationInventors: Xiongwei Yan, Pau-Miau Yuan, Sylvia Yuen, Kuo-Liang Hsi, Joe Lam, Krishna Upadhya, Subhaker Dey, Darryl Pappin
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Patent number: 6979530Abstract: Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis.Type: GrantFiled: May 21, 2001Date of Patent: December 27, 2005Assignee: Applera CorporationInventors: Xiongwei Yan, Sheri Miraglia, Pau Miau Yuan
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Patent number: 6787030Abstract: The present invention includes a method of analyzing one or more molecular components in a mixture of components. In the method, a mixture of molecular components, i.e., an analyte mixture, is separated on a capillary liquid chromatography column. The component-containing eluate from the column is deposited as a series of discrete, defined-volume microdrops, along a region of an adsorbent collection layer. During the chromatographic separation, the column eluate may also-be monitored to detect the presence of separated components in the equate. The one or more components deposited in the collection layer are then analyzed by selected analytical techniques. In related aspect, the invention includes a method of collecting one or more molecular components derived from a mixture of components, a blotter apparatus useful in the above method, and a system for analyzing one or more molecular components in a mixture of components.Type: GrantFiled: June 23, 2003Date of Patent: September 7, 2004Assignee: Applera CorporationInventors: Kuo-Liang Hsi, Jindong Zhao, Michael Kochersperger, William E. Werner, Pau-Miau Yuan
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Publication number: 20040060872Abstract: The present invention includes a method of analyzing one or more molecular components in a mixture of components. In the method, a mixture of molecular components, i.e., an analyte mixture, is separated on a capillary liquid chromatography column. The component-containing eluate from the column is deposited as a series of discrete, defined-volume microdrops, along a region of an adsorbent collection layer. During the chromatographic separation, the column eluate may also-be monitored to detect the presence of separated components in the equate. The one or more components deposited in the collection layer are then analyzed by selected analytical techniques. In related aspect, the invention includes a method of collecting one or more molecular components derived from a mixture of components, a blotter apparatus useful in the above method, and a system for analyzing one or more molecular components in a mixture of components.Type: ApplicationFiled: June 23, 2003Publication date: April 1, 2004Applicant: Applera CorporationInventors: Kuo-Liang Hsi, Jindong Zhao, Michael Kochersperger, William E. Werner, Pau-Miau Yuan
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Publication number: 20030186214Abstract: Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis.Type: ApplicationFiled: May 21, 2001Publication date: October 2, 2003Inventors: Xiongwei Yan, Sheri Miraglia, Pau Miau Yuan
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Publication number: 20030158407Abstract: Fluorescent, sulfonated 3,7-diamino-[8,9]benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a variety of contexts, including cell surface assays employing intact, live cells and in nucleic acid detection methods. The new dyes are water soluble and can be conjugated to a variety of substrates, such as polynucleotides, nucleosides, nucleotides, peptides, proteins, antibodies, carbohydrates, ligands, particles and surfaces.Type: ApplicationFiled: October 8, 2002Publication date: August 21, 2003Applicant: Applera CorporationInventors: Xiongwei Yan, Pau Miau Yuan
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Patent number: 6582597Abstract: The present invention includes a method of analyzing one or more molecular components in a mixture of components. In the method, a mixture of molecular components, i.e., an analyte mixture, is separated on a capillary liquid chromatography column. The component-containing eluate from the column is deposited as a series of discrete, defined-volume microdrops, along a region of an adsorbent collection layer. During the chromatographic separation, the column eluate may also be monitored to detect the presence of separated components in the eluate. The one or more components deposited in the collection layer are then analyzed by selected analytical techniques. In related aspect, the invention includes a method of collecting one or more molecular components derived from a mixture of components, a blotter apparatus useful in the above method, and a system for analyzing one or more molecular components in a mixture of components.Type: GrantFiled: June 19, 2001Date of Patent: June 24, 2003Assignee: Applera CorporationInventors: Kuo-Liang Hsi, Jindong Zhao, Michael Kochersperger, William E. Werner, Pau-Miau Yuan
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Patent number: 6465644Abstract: Fluorescent, sulfonated 3,7-diamino-[8,9]benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a variety of contexts, including cell surface assays employing intact, live cells and in nucleic acid detection methods. The new dyes are water soluble and can be conjugated to a variety of substrates, such as polynucleotides, nucleosides, nucleotides, peptides, proteins, antibodies, carbohydrates, ligands, particles and surfaces.Type: GrantFiled: May 2, 2000Date of Patent: October 15, 2002Assignee: Applera CorporationInventors: Xiongwei Yan, Pau Miau Yuan
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Publication number: 20020011444Abstract: The present invention includes a method of analyzing one or more molecular components in a mixture of components. In the method, a mixture of molecular components, i.e., an analyte mixture, is separated on a capillary liquid chromatography column. The component-containing eluate from the column is deposited as a series of discrete, defined-volume microdrops, along a region of an adsorbent collection layer. During the chromatographic separation, the column eluate may also be monitored to detect the presence of separated components in the equate. The one or more components deposited in the collection layer are then analyzed by selected analytical techniques. In related aspect, the invention includes a method of collecting one or more molecular components derived from a mixture of components, a blotter apparatus useful in the above method, and a system for analyzing one or more molecular components in a mixture of components.Type: ApplicationFiled: June 19, 2001Publication date: January 31, 2002Applicant: The Perkin-Elmer CorporationInventors: Kuo-Liang Hsi, Jindong Zhao, Michael Kochersperger, William E. Werner, Pau-Miau Yuan