Abstract: A regeneration medium for use in wheat transformation which utilises copper at a concentration in a range from greater than 50 &mgr;M to less than or equal to 300 &mgr;M and a growth hormone at a concentration in a range from 0.05 to 10 mg l−1 are disclosed. Growth hormone is added to the regeneration medium if wheat tissue or cell has not been treated with a growth hormone after callus induction and prior to using the wheat regeneration medium. A method for producing a transformed wheat plant using this regeneration medium is also disclosed.

Abstract: A method for somatic embryogenesis of soybean, (Glycine max), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/l. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly embryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed.Methods for transforming somatic tissue from soybean and other Glycine species are also provided.Whole, fertile, transformed plants are obtained.